Navigation Links
Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation

er dye concentrations), ratiometric dyes offer several advantages over conventional single-wavelength indicators:

Benefits of using the 340/380 nm excitation ratios for Fura-2:
Signal is not dependent on:
-Dye concentration
-Illumination intensity
-Optical path length

The effects of uneven dye loading, dye leakage and photo bleaching are minimized
-Detection can usually be made over a period of 1 hour without significant loss of fluorescence

A more stable signal and better signal-to-noise ratio may be obtained
- Increased sensitivity

Accurate measurements of the intracellular Ca2+ concentration is possible

An alternative approach is a series of no-wash reagents available from Molecular Devices, such as the FlexStation Calcium Assay Kit. These, too, confer a number of potential advantages over conventional Ca2+ indicators.

Problems in a conventional assay protocol that may be reduced with no-wash reagents:
Cells removed from the plates during the washing procedure
Reduced responsiveness (competence) of the cells following washing
Variation in the residual volume of wash buffer, leading to variation in the concentration of test compound
The drop in fluorescence (resulting from the dilution of residual extracellular dye) upon addition of the test compound

In this application note we demonstrate the use of both single-wavelength and dual-wavelength Ca2+ indicators in the FlexStation benchtop scanning fluorometer and the possible benefits of a homogeneous assay system.


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Versatile Reporter Vectors for Monitoring Viral Transduction
2. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
3. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
4. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
5. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
6. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
7. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
8. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
9. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
10. Oil-Free PCR Using the Hot Top Assembly
11. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
Post Your Comments:
(Date:7/22/2014)... from the National Institute of Standards and Technology ... demonstrated a new design for an atomic clock ... or a microcomb. , The microcomb clock, featured ... the new journal Optica ,* is the ... and its accurate conversion of optical frequencies to ...
(Date:7/22/2014)... July 22, 2014 Nuclear reactors are ... produced by controlled fission chain reaction in nuclear reactors ... is used to drive a power- generating turbine. Currently ... the world’s total electricity production. Nuclear reactor market is ... construction, planned and proposed nuclear reactor projects. , Early ...
(Date:7/22/2014)... The DNA Spectrum Ancestry Map provides ... history, migration, and culture. , Throughout his childhood ... stories stemming from Europe. His father passionately referred to ... 8mm home movies painted an elaborate picture of an ... ordered a test from DNA Spectrum to expand upon ...
(Date:7/22/2014)... CAMBRIDGE, Massachusetts og SHENZHEN ... BGI Tech Solutions Co., LTD, (BGI Tech), et ... globale lancering af en ny human whole exome-sekvenseringstjeneste ... Complete Genomics, højt anset i branchen for ... og højfølsom registrering af alle typer varianter, blev ...
Breaking Biology Technology:'Comb on a chip' powers new NIST/Caltech atomic clock design 2Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 2Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 3DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 2DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 3DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 4BGI Tech lancerer Human Whole Exome-sekvenseringstjeneste på Complete Genomics avancerede platform 2
... Oncothyreon Inc.,(Nasdaq: ONTY ) (TSX: ONY) ... office has issued a patent for PX-867, ... The newly issued patent, U.S. Patent,# 7,335,679, ... of PX-867,and pharmaceutical preparations containing PX-867., ...
... Secretary Leavitt to present the Chartered Value Exchange designation ... during March 20 visit to Portland, PORTLAND, Ore., ... improve care and physician practices in Oregon,The Oregon Health ... Exchange designation from the U.S. Department of Health and,Human ...
... (Nasdaq: BPUR ) announced today that ... Agency (MHRA) has completed,its inspection of the ... no "Critical" and no "Major" deficiencies in,compliance ... identified,shortcomings characterized as "Other," with a request ...
Cached Biology Technology:Oncothyreon announces issuance of patent for PX-867 2Oncothyreon announces issuance of patent for PX-867 3Oregon Health Care Quality Corporation Receives Important Designation from U.S. Department of Health and Human Services 2Biopure Completes U.K. Regulatory Agency Inspection 2
(Date:7/22/2014)... New research from the Monell Center reveals that simply ... airway inflammation in asthmatics for at least 24 hours ... can play in health-related outcomes. , "Asthmatics often are ... an odor is harmful, our bodies react as if ... Cristina Jan, PhD, a Monell physiologist. "Both patients and ...
(Date:7/22/2014)... the average steak, but it seems to help wood ... winter cold. , "Alaska wood frogs spend more time ... your freezer and the frog comes back to life ... said Don Larson, University of Alaska Fairbanks graduate student ... freeze tolerance in Alaska wood frogs is more extreme ...
(Date:7/22/2014)... dust mites (and chances are you are), help may ... of Iowa have developed a vaccine that can combat ... In animal tests, the nano-sized vaccine package lowered lung ... allergens, according to the paper, published in the ... . One big reason why it works, the researchers ...
Breaking Biology News(10 mins):In asthma, it's not just what you smell, but what you think you smell 2In asthma, it's not just what you smell, but what you think you smell 3Alaska frogs reach record lows in extreme temperature survival 2Alaska frogs reach record lows in extreme temperature survival 3Researchers create vaccine for dust-mite allergies 2
... Today, a new, international competition called the Digging ... research agencies: the Joint Information Systems Committee (JISC) ... the Humanities (NEH) and the National Science Foundation ... Sciences and Humanities Research Council (SSHRC) from Canada. ...
... VIRGINIA KEY, Fla. -- An international team of scientists ... for decades. They have discovered that fish contribute a ... affects the delicate pH balance of seawater. The study ... of marine calcium carbonate being produced by fish, but ...
... in the Arctic is happening at a greater rate ... is expected to continue in the future. As ... erosion and sea level rise can be expected to ... Arctic climates demonstrates for the first time the pervasive ...
Cached Biology News:Leading research agencies announce new international competition: "The digging into data challenge" 2Fishdunnit! Mystery solved 2Fishdunnit! Mystery solved 3Arctic heats up more than other places 2
Contains L-glutamine....
Contains L-glutamine...
... Clone/PAD: ZMD.358. Immunogen: ... C-terminal region of human mouse ... Reacts with human and mouse ... Human (positive controls: mouse LRP5-pSEC-tag-transfected ...
Replacement Parts for Electrophoresis Apparatus...
Biology Products: