Navigation Links
Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System

on population levels to be evaluated for a wide variety of microorganisms.

The use of real-time qPCR in environmental microbiology is a novel and potentially powerful application of molecular biology to environmental science. The high degree of accuracy and robustness of the real-time qPCR technique combined with the advantages of high throughput and reduced assay costs make this method invaluable for the analysis of microbial populations.

References
1. DeLong, E.F. Archaea in coastal marine environments. Proc. Natl. Acad. Sci. 89: 5685- 5689 (1992).

2. Giovannoni, S.J., Britschgi, T.B., Moyer, C.L., and Field, K.G. Genetic diversity in Sargasso Sea bacterioplankton. Nature 345: 60-63 (1990).

3. Wagner, M.A., Roger, A.J., Flax, J.L., Brusseau, G.A., and Stahl, D.A. Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration. J. Bacteriol. 180: 2975-2982 (1998).

4. Shaw, M.R., Zavaleta, E.S., Chiariello, N.R., Cleland, E.E., Mooney, H.A., and Field, C.B. Grassland responses to global environmental changes suppressed by elevated CO2. Science. 298:1987-1990 (2002).

5. Hollocher, T. C., Tate, M. E., and Nicholas, D. J. Oxidation of ammonia by Nitrosomonas europaea: definitive 18O-tracer evidence that hydroxylamine formation involves a monooxygenase. J. Biol. Chem. 256:1083410836 (1981).

6. Morrison, T.B., Weis, J.J., and Wittwer, C.T. Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification. Biotechniques 24: 954-958 (1998).

7. Horz, Hans-Peter and Bohannan, Brendan. (submitted).

8. Optimal plate read temperature for real-time PCR with SYBR Green I. MJ Research Technical Note
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Versatile Reporter Vectors for Monitoring Viral Transduction
2. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
3. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
4. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
5. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
6. Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation
7. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
8. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
9. Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
10. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
11. Oil-Free PCR Using the Hot Top Assembly
Post Your Comments:
(Date:4/28/2015)... SAN FRANCISCO , April 28, 2015 /PRNewswire/ ... focused on the development and commercialization of therapies ... today announced the completion of a Series A ... million with potential tranches totaling an additional $1.5 ... Morningside Venture.  Other investors include a consortium of ...
(Date:4/28/2015)... Denmark , April 28, 2015  DanDrit Biotech ... biotechnology company developing a vaccine for the treatment of ... signed a final collaboration agreement with GISCAD Foundation, a ... tract cancer studies. The collaboration relates to ... in patients with no evidence of disease (NED) Stage ...
(Date:4/28/2015)... Md. and RESEARCH TRIANGLE PARK, N.C., April 28, 2015 ... ) today announced its financial results for the ... total revenues increased as compared to the prior ... growing number of patients being prescribed our pulmonary ... United Therapeutics, Chairman and Co-Chief Executive Officer. "Since ...
(Date:4/28/2015)... , April 28, 2015  US-Australian drug discovery company, ... that it received a letter from NASDAQ informing it ... Rule 5550(b) (Listing Rule). In November ... requesting the Company to submit a plan to regain ... a minimum of $2,500,000 in stockholders, equity as of ...
Breaking Biology Technology:InCarda Therapeutics Completes Over $5 Million Private Financing 2DanDrit Biotech USA, Inc. Announces Final Collaboration Agreement with the GISCAD Foundation 2DanDrit Biotech USA, Inc. Announces Final Collaboration Agreement with the GISCAD Foundation 3DanDrit Biotech USA, Inc. Announces Final Collaboration Agreement with the GISCAD Foundation 4United Therapeutics Corporation Reports First Quarter 2015 Financial Results 2United Therapeutics Corporation Reports First Quarter 2015 Financial Results 3United Therapeutics Corporation Reports First Quarter 2015 Financial Results 4United Therapeutics Corporation Reports First Quarter 2015 Financial Results 5United Therapeutics Corporation Reports First Quarter 2015 Financial Results 6United Therapeutics Corporation Reports First Quarter 2015 Financial Results 7United Therapeutics Corporation Reports First Quarter 2015 Financial Results 8United Therapeutics Corporation Reports First Quarter 2015 Financial Results 9United Therapeutics Corporation Reports First Quarter 2015 Financial Results 10Novogen Regains Full Compliance with NASDAQ Listing Rule 2Novogen Regains Full Compliance with NASDAQ Listing Rule 3
... -- Advisor to President Barack Obama for the Office ... visited the Navy,s corporate laboratory, March 16, to dedicate ... (LASR) and tour the sprawling 130-acre Washington, D.C., campus. ... Navy, Marine Corps and our Nation in ever evolving ...
...  Foundation for Health Improvement and Technology (FHIT), ... nonprofit, with the mission to rid the world ... educational programs promoting early detection and aggressive medical ... have made it entirely possible to detect many ...
... 2012More than half of likely voters doubt that the ... in science, technology and health care by the year ... commissioned by Research!America. The findings reveal deep concerns among ... status in innovation, research and development before the next ...
Cached Biology Technology:White House S&T advisor the Honorable Dr. John Holdren tours Naval Research Laboratory 2Foundation for Health Improvement and Technology (FHIT) Launched to Beat Cardiovascular Disease 2More than half of Americans doubt US global leadership in 2020 2
(Date:4/27/2015)... 2015  For more than four decades, AUVSI,s ... the premier worldwide event for the unmanned land, sea, ... event for any local, national, or trade news organization ... markets and current applications of unmanned technologies. The conference ... $48 billion industry, and how it will soon impact ...
(Date:4/27/2015)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" ... the growing mobile commerce market, announces that its Wocket® ... first week of May, 2015 and will be fulfilling ... Gino Pereira , Chief Executive Officer ... as Wocket® enters the consumer market. We would like ...
(Date:4/27/2015)... , Apr. 27, 2015 Profile Solutions, Inc. ... and security systems is pleased to announce that Dr ... a consultant and member of its scientific advisory board. ... known as a thought leader in technology-enhanced learning models. ... Group, and has published studies and books focused on ...
Breaking Biology News(10 mins):UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 3UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 4UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 5Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 2Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 3Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 2Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 3Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 4
... flight routes of the birds, postgraduate Matthias Kopp, under the ... in their breeding areas on King George Island, about 120 ... their position data over a period of several years, followed ... from Switzerland. "With the help of these data we can ...
... Md. A team of scientists have used the Ozone ... reductions in the levels of a key air pollutant generated ... pollutant, sulfur dioxide, contributes to the formation of acid rain ... led by an Environment Canada researcher, have shown that sulfur ...
... atheist scientists with children embrace religious traditions for social ... and the University at Buffalo -- The State University ... found that some atheist scientists want their children to ... informed decisions about their own religious preferences. "Our ...
Cached Biology News:Where Antarctic predatory seabirds overwinter 2NASA satellite confirms sharp decline in pollution from US coal power plants 2NASA satellite confirms sharp decline in pollution from US coal power plants 3Some atheist scientists with children embrace religious traditions, according to new Rice research 2
Thermo Labsystems offers a basic reader for 96-well microplates that is especially adapted for ELISA and other colorimetric assays....
Abgene's ALTO-8 is the first automated decapper/capper for Abgene Twist-Lock tubes....
Abgene's ALTO-8 is the first automated decapper/capper for Abgene Twist-Lock tubes....
... Protease is an enhanced form of Tobacco ... site-specific, highly active, and significantly more stable ... activity. AcTEV Protease specifically recognizes a seven ... Gly), making it useful for removing affinity ...
Biology Products: