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Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System

on population levels to be evaluated for a wide variety of microorganisms.

The use of real-time qPCR in environmental microbiology is a novel and potentially powerful application of molecular biology to environmental science. The high degree of accuracy and robustness of the real-time qPCR technique combined with the advantages of high throughput and reduced assay costs make this method invaluable for the analysis of microbial populations.

References
1. DeLong, E.F. Archaea in coastal marine environments. Proc. Natl. Acad. Sci. 89: 5685- 5689 (1992).

2. Giovannoni, S.J., Britschgi, T.B., Moyer, C.L., and Field, K.G. Genetic diversity in Sargasso Sea bacterioplankton. Nature 345: 60-63 (1990).

3. Wagner, M.A., Roger, A.J., Flax, J.L., Brusseau, G.A., and Stahl, D.A. Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration. J. Bacteriol. 180: 2975-2982 (1998).

4. Shaw, M.R., Zavaleta, E.S., Chiariello, N.R., Cleland, E.E., Mooney, H.A., and Field, C.B. Grassland responses to global environmental changes suppressed by elevated CO2. Science. 298:1987-1990 (2002).

5. Hollocher, T. C., Tate, M. E., and Nicholas, D. J. Oxidation of ammonia by Nitrosomonas europaea: definitive 18O-tracer evidence that hydroxylamine formation involves a monooxygenase. J. Biol. Chem. 256:1083410836 (1981).

6. Morrison, T.B., Weis, J.J., and Wittwer, C.T. Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification. Biotechniques 24: 954-958 (1998).

7. Horz, Hans-Peter and Bohannan, Brendan. (submitted).

8. Optimal plate read temperature for real-time PCR with SYBR Green I. MJ Research Technical Note
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