Navigation Links
Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System

(for determining C(t)) was set manually to 0.015 fluorescence units for all experiments, using the Opticon Monitor software (MJ Research). This threshold is automatically applied to all wells for consistent analysis of individual samples and standards.

Quantification Analysis
All samples were analyzed in triplicate. The cycle threshold (C(t)) values for amoA were determined for each sample and for the control plasmid- DNA template. The amoA target was normalized to the plasmid-DNA control using the following calculation: ΔC(t)sample = average-C(t)amoA average-C(t)plasmid-DNA. The normalized sample C(t) values were then referenced to one soil sample (the calibrator) to determine the relative effect of the environmental treatments on AOB levels. The formula used for determining relative amoA levels is as follows: ΔΔC(t)sample = ΔC(t)sample ΔC(t)calibrator. The amount of amoA for each soil sample is reported as a ratio relative to the calibrator using the following formula: 2ΔΔC(t) (ref. 9).


Results
Relative Quantification of amoA Levels for Soil Plots
Each of the 16 samples presented in this report was subjected to a different combination of environmental factors: temperature, CO2, precipitation, and nitrogen deposition. Table 3 shows the mean C(t) standard deviation as well as successive calculations for relative quantification of the amoA gene in four representative samples. Note that sample 1 has been designated as the calibrator to which the other three samples were normalized. The standard deviation for triplicate measures of each sa
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Versatile Reporter Vectors for Monitoring Viral Transduction
2. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
3. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
4. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
5. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
6. Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation
7. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
8. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
9. Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
10. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
11. Oil-Free PCR Using the Hot Top Assembly
Post Your Comments:
(Date:10/20/2014)... 20, 2014 , A major ... and the delivery of care has revealed changing trends ... inequalities in the provision of healthcare services across the ... by United European Gastroenterology (UEG), have been announced today ... awareness of the burden of GI disorders across ...
(Date:10/20/2014)... Local veterinary surgeon, Dr. Tim McCarthy is ... donor stem cells for dogs with osteoarthritis. Dr. McCarthy ... performed clinical stem cell therapy for 7 years. The ... a single injection of donor stem cells into one ... and inflammation in the treated joints. , Candidates ...
(Date:10/19/2014)... Asian Automatic patient billing report defines and segments the ... The Automatic patient billing market in Asia is estimated ... a developing CAGR of 7.2% from 2013 to 2018. ... patient billing market, to get an idea of the ... the segmentation of this market in the same region, ...
(Date:10/19/2014)... 2014 According to market research ... WLAN Controllers; Wireless gateways)]- Global Advancements, Forecasts and ... global Outdoor Wi-Fi market with analysis and forecasting ... also identifies the drivers and the restrains for ... opportunities and challenges. , Browse 65 market data ...
Breaking Biology Technology:Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 2Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 3Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 4Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 5Cascade Veterinary Referral Center Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2The Asian Automatic patient billing market is estimated to grow to around $463.9 million by 2018 - New Report by MicroMarket Monitor 2The Asian Automatic patient billing market is estimated to grow to around $463.9 million by 2018 - New Report by MicroMarket Monitor 3Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 2Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 3Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 4
... venture capital in more deals in 2006, but the improvement ... Ernst & Young, LLP and Dow Jones ... in 2006, including a $12 .5 million round by ... for a total of $58.47 million. The total, up from ...
... Lance Armstrong is a unique phenomenon. He's a talented athlete, ... league by himself. Or is he? , ,As many people know, ... National Center for Health Statistics report released by the ... the second year in a row. , ,Cancer patients as political ...
... director of technology programs for the University of Wisconsin-Madison, is ... he demonstrates in Part II of this interview. In ... In Part II (below), he addresses creating a culture of ... also is a featured speaker at the Fusion 2007 ...
Cached Biology Technology:Wisconsin fares slightly better in venture capital chase 2Wisconsin fares slightly better in venture capital chase 3Lance Armstrong and the future of cancer care 2Lance Armstrong and the future of cancer care 3Lance Armstrong and the future of cancer care 4Lance Armstrong and the future of cancer care 5
(Date:10/14/2014)... JOLLA, CA – October 14, 2014 – Scientists from ... million from the National Institutes of Health (NIH) to ... prevalent virus-induced hemorrhagic fever disease in Africa. The study ... and why some patients die, while others survive the ... to understand the basic mechanism of how Lassa fever ...
(Date:10/14/2014)... team of researchers, led by the Chinese Academy of ... Nature Genetics a brief genomic history of tomato ... tomato plant. , The C.M. Rick Tomato Genetics Resource ... this study by providing seed of both cultivated tomato ... builds on the first tomato genome sequence completed just ...
(Date:10/14/2014)... October 14, 2014 – Over the past decades, we ... institutions to meet the needs of infants for social ... these infants to thrive. , Infancy and ... of the cortex. A generation of research suggests that ... cortical development and cognitive function. In contrast, deprivation and ...
Breaking Biology News(10 mins):Scripps Research Institute team receives $6.6 million to investigate deadly Lassa virus 2Institutional rearing may increase risk attention-deficit disorder 2
... YORK (APRIL 15, 2008) A comprehensive book of essays, ... released today by the Wildlife Conservation Society. ... such issues as diseases shared by animals and people; and ... Afghanistan. State of the Wild: A Global Portrait of ...
... there? Probably not, according to a scientist from the University ... Andrew Watson suggests that the odds of finding new life ... has taken for beings such as humans to evolve and ... and intelligent life evolved late on Earth and it has ...
... worlds oldest recorded tree is a 9,550 year old spruce in ... shown to be a tenacious survivor that has endured by growing ... climate changes over time. For many years the spruce ... mountain region. Our results have shown the complete opposite, ...
Cached Biology News:Wildlife Conservation Society releases definitive 'state of the wild' 2Is there anybody out there? 2World's oldest living tree discovered in Sweden 2
... demands of the routine trace metal analytical ... robust and simple to use instrumentation. The ... most challenging sample matrices found in the ... the toughest analytical challenges found in the ...
... Do you need to make your strain ... efficiency? Take advantage of Lucigen’s unrivalled expertise ... Competent Cell Service offers: Chemically ... efficiencies Fast turnaround ...
Procollagen type I C-terminal propeptide (human, PICP)...
... in Microarrays: Biomedical Photometrics Inc. (Waterloo, Ontario, ... Germany) have collaborated to develop a fluorescence ... The DNAscope AT is based on ... AT automatically reads up to six ArrayTubes ...
Biology Products: