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Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis

ng PCR products in adjacent lanes, we were able to compare band profiles and, hence, genetic diversity from different environmental samples. Additionally, sequencing the gel-resolved DNA enables phylogenetic analysis of PCR products, eliminating the need to clone DNA fragments and, thus. simplifying our analysis.


References
1. Hastings, R. C. et al., Appl. Environ. Microbiol., 64, 36743682 (1998).

2. Hastings, R. C. et al., FEMS Microb. Ecol., 23, 4554 (1997).

3. Muyzer, G. et al., Appl. Environ. Microbiol., 59, 695700 (1993).

4. Head, I. M. et al., J. Gen. Microbiol., 139, 11471153 (1993).

5. Fischer, S. and Lerman, L. Methods in Enzymol., 68, 183191 (1979).

6. Zoller, P. et al., Bio-Rad Bulletin, 2408, (1998).

* The Polymerase Chain Reaction (PCR) Process is covered by patents owned by Hoffman-La Roche. Use of the PCR process requires a license.


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