ng PCR products in adjacent lanes, we were able to compare
band profiles and, hence, genetic diversity from different environmental
samples. Additionally, sequencing the gel-resolved DNA enables phylogenetic
analysis of PCR products, eliminating the need to clone DNA fragments
and, thus. simplifying our analysis.
1. Hastings, R. C. et al., Appl. Environ. Microbiol., 64, 36743682
2. Hastings, R. C. et al., FEMS Microb. Ecol., 23, 4554 (1997).
3. Muyzer, G. et al., Appl. Environ. Microbiol., 59, 695700 (1993).
4. Head, I. M. et al., J. Gen. Microbiol., 139, 11471153 (1993).
5. Fischer, S. and Lerman, L. Methods in Enzymol., 68, 183191
6. Zoller, P. et al., Bio-Rad Bulletin, 2408, (1998).
* The Polymerase Chain Reaction (PCR) Process is covered by patents owned
by Hoffman-La Roche. Use of the PCR process requires a license.
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. Versatile Reporter Vectors for Monitoring Viral Transduction2
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green fluorescent protein (GFP) detection
with the Agilent 2100 bioanalyzer3
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. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS5
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