Navigation Links
Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis

sterile scalpel, eluted by passive diffusion into 1.25x TAE and sequenced.


Results and Discussion
TTGE analysis of freshwater Nitrosospira 16S rDNA PCR products revealed both genotypic continuity and variation between individual samples (Figure 1). Co-migrating DNA bands (position B) were observed during the periods of lakewater overturn and stratification, suggesting the presence of a predominant Nitrosospira genotype during these times. Spring and autumn samples, corresponding to formation and breakdown of a stratified water column respectively, demonstrated the predominance of other strains of Nitrosospira by the observation of differentially migrating PCR products (positions A, C, D, E and F). While myriad other DNA bands present throughout the gel indicated an overall complex genetic profile within this Nitrosospira group, the relative quantity of DNA in individual bands suggested the numerical dominance by a number of strains (positions A to F) whose prevalence appeared to be temporally variable. Six DNA bands (positions A to F) were excised from the gel and sequenced. Phylogenetic analysis of these sequences grouped them as closely-related genotypes, but in a separate cluster were grouped to previously recognized Nitrosospira spp.4

Confidence in dendrogram topology is limited due to the restricted quantity of sequence data available for scrutiny (approx. 200 bp), yet the acute resolving power of the TTGE technique enabled the separation of Nitrosospira 16S rRNA genes differing by as little as one base pair across the fragment examined.

TTGE is a reliable technique and a valuable tool for microbial diversity studies. By loadi
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Versatile Reporter Vectors for Monitoring Viral Transduction
2. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
3. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
4. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
5. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
6. Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation
7. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
8. High-Throughput Cultivation of Bacterial Clones
9. Mammalian and Bacterial Expression in One Vector
10. Bacterial mRNA Isolation, Fast and Easy
11. Bacterial RNA Isolation From Infected Eukaryotic Hosts
Post Your Comments:
(Date:10/22/2014)... and HONG KONG , ... rare disease therapeutics enterprise, announced today that rare disease ... company as vice president, research. Dr. McKew brings more ... key leadership positions at the National Institutes of Health, ... acquisition by Wyeth). Dr. McKew will lead aTyr,s efforts to ...
(Date:10/20/2014)... GUELPH, ON , Oct. 20, 2014 /PRNewswire/ - BIOREM Inc. ... totalling $1.7 million which brings Q3 orders to $5.8 million and ... odour control projects in North America and ... "We continue to bid new projects at record levels," said ... values were higher at the end of Q3 than they have ...
(Date:10/20/2014)... October 20, 2014 Earle ... LLC, announced today that Ellen Teplitzky, an experienced ... for the pharmaceutical industry, has joined the firm ... its legal services practice. NDA Partners provides ... witness and testimony, to top law firms and ...
(Date:10/19/2014)... The Asia-Pacific Bromine Market report defines ... analysis and forecast of revenue. , Browse through ... report, to get an idea of the in-depth ... the segmentation in the Asia-Pacific bromine market, and ... , http://www.micromarketmonitor.com/market/asia-pacific-bromine-6741503144.html , Bromine is a ...
Breaking Biology Technology:aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3NDA Partners Appoints Ellen Teplitzky, JD as Director of its Legal Services Practice 2The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 3
... , CALGARY and BLENHEIM, Ontario, Nov. 24 Dow AgroSciences ... Seeds, a division of Thompsons Limited of Blenheim, Ontario. The ... seeds business as the company anticipates the introduction of ... AgroSciences Herbicide Tolerant Trait Technology in corn in 2012. The ...
... , SHANGHAI, Nov. 24 ... Trademark Office (PTO) has allowed the patent application for,its ... NOD technology is a highly efficient approach ... oral delivery of drugs,otherwise administered as injections. NOD technology ...
... , , Primary efficacy endpoint has ... recurrent endometrial cancer, , QUEBEC CITY, Nov. 24 /PRNewswire-FirstCall/ - ... "Company"), a global biopharmaceutical company focused on endocrine therapy and ... study with its targeted cytotoxic peptide conjugate, AEZS-108 (formerly AN-152), ...
Cached Biology Technology:Dow AgroSciences Canada Announces Agreement to Acquire Hyland Seeds 2Dow AgroSciences Canada Announces Agreement to Acquire Hyland Seeds 3AEterna Zentaris Announces Positive Results for Phase 2 Study with LHRH-Receptor Targeted Cytotoxic Conjugate AEZS-108 in Endometrial Cancer 2AEterna Zentaris Announces Positive Results for Phase 2 Study with LHRH-Receptor Targeted Cytotoxic Conjugate AEZS-108 in Endometrial Cancer 3AEterna Zentaris Announces Positive Results for Phase 2 Study with LHRH-Receptor Targeted Cytotoxic Conjugate AEZS-108 in Endometrial Cancer 4
(Date:10/18/2014)... October 2014 Psychological stress and stress-related psychiatric disorders ... diseases, but the molecular mechanisms underlying this ... contribute to the development of targeted preventive ... these devastating diseases. This work is presented ... in Berlin., Now an international group of ...
(Date:10/17/2014)... the University of Copenhagen have shown for the first ... of Cystic fibrosis patients, giving them the opportunity to ... chronic infections. , The study also discovered the bacterial ... patients was halted or slowed down by the immune ... the oxygen and helped "suffocate" the bacteria, forcing the ...
(Date:10/16/2014)... on how the bowel lining develops and, in the ... starts. , The researchers produced evidence that stem cells ... are a feature of the bowel lining, and believe ... a controversial finding as scientists are still divided on ... Dr Chin Wee Tan and Professor Tony Burgess from ...
Breaking Biology News(10 mins):Researchers find why depression and aging linked to increased disease risk 2Scientists opens black box on bacterial growth in cystic fibrosis lung infection 2Cryptic clues drive new theory of bowel cancer development 2
... Today sees the launch of Ensembl Plants ... research by the European Molecular Biology Laboratory,s ... Cold Spring Harbor Laboratory, USA. Ensembl Plants allows ... of genome-scale experiments in different plant species. By ...
... good, more must be better, right? Wrong! New research published ... shows that vitamin A plays a crucial role in energy ... has a complex negative effect on our bodies. This is ... supplements containing added vitamin A make an overdose more possible ...
... calorie intake or glance over nutritional information on food packages, ... Dr. Susan Nelson, a veterinarian and assistant professor of clinical ... to follow when feeding your pet to ensure that it ... cat and dog food packages contain nutritional information, Nelson said. ...
Cached Biology News:New portal for plant genomics will support research into improved crops 2Too much of a good thing? Scientists explain cellular effects of vitamin A overdose and deficiency 2Owners should count calories for obese pets, consider several factors for good health 2Owners should count calories for obese pets, consider several factors for good health 3
... I, Wild Type Liquid. In 100 ... 200 µM EDTA, 20% glycerol, pH 7.9. AVOID ... is a monomeric protein of 765 amino acids ... relaxation of both positive and negative supercoiled DNAs ...
Request Info...
... in a 3'-> 5' direction from a ... of single-stranded DNA. Under defined reaction conditions, ... a uniform rate yielding predictable and reproducible ... of deoxyribonucleotides by Exonuclease III is dependent ...
... an endoribonuclease, the RNA strand of ... origin, e.g. that of phage FX174, ... x poly(dT). RNase H produces ribonucleotides ... no activity is detected with polyribonucleotides ...
Biology Products: