Navigation Links
Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis

and sequence analysis of gel-resolved DNA further permits phylogenetic interpretation of a microbial community structure that may be correlated with environmental factors.

Ammonia-oxidizing bacteria mediate the nitrification stage of the nitrogen cycle, hence their activity is of ecological importance, but the influence of environmental parameters upon their community structure is largely unknown.1 Described here is the combined application of TTGE and sequence analysis of gel-resolved 16S rDNA PCR products amplified from freshwater Nitrosospira spp. collected throughout a twelve month period in an attempt to observe temporal genetic variation within this functionally important microbial group.


Materials and Methods
Genomic DNA, extracted from lakewater samples collected at monthly intervals from January to December, was PCR-amplified to generate 240 bp GC-clamped fragments of the 16S rRNA gene spanning the variable V3 region from ammonia-oxidizing bacteria belonging to the genus Nitrosospira.2,3 Approximately 200 ng of PCR product were mixed with 2x bromophenol blue loading dye and electrophoresed in a 6% polyacrylamide gel (acrylamide/bis 37.5:1) containing 7 M urea, 20% formamide, 2% glycerol in 1.25x TAE buffer (50 mM Tris-Cl, 25 mM acetic acid, 1.25 mM EDTA, pH 8.0) using the DCode system at 100 V for 16 hr across a temperature range of 4055 C and a temperature ramp of 1 C/hr. After electrophoresis, gels were equilibrated in 1.25x TAE buffer for 15 min., stained in ethidium bromide (25 mg/ml in TAE) for 20 min. and washed in TAE before viewing and photographing under UV light (302 nm). Various individual DNA bands were cut from the gel with a
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Versatile Reporter Vectors for Monitoring Viral Transduction
2. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
3. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
4. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
5. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
6. Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation
7. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
8. High-Throughput Cultivation of Bacterial Clones
9. Mammalian and Bacterial Expression in One Vector
10. Bacterial mRNA Isolation, Fast and Easy
11. Bacterial RNA Isolation From Infected Eukaryotic Hosts
Post Your Comments:
(Date:7/31/2014)... Calif. (PRWEB) July 31, 2014 Held ... diagnosed patients with an overview of the disease and ... of drug side effects, as well as updates on ... grateful to Dr. Sandy Srinivas for organizing this educational ... of kidney cancer. “Sandy and her colleagues have put ...
(Date:7/31/2014)... (PRWEB) July 31, 2014 Rancho ... the integration of Omicsoft’s Array Suite with tranSMART ... will be able to send data in tranSMART to ... with all versions of tranSMART including the new 1.2 ... statistics, visualization and storage for the analysis of high ...
(Date:7/31/2014)... The global market for molecular diagnostics is expected ... a new study by Grand View Research, Inc. Growing ... introduction of advanced cancer diagnostic technologies are expected to ... six years. Moreover, the growing global base of geriatric ... disease triggering lifestyle habits such as smoking and excessive ...
(Date:7/31/2014)... Although RNA-Sequencing is a relatively new ... appearing around 2008, RNA-Sequencing has rapidly evolved to ... the last several years, assays have improved to ... input amounts, process degraded RNA, and perform well ... bioinformatics side, there have been improvements in references, ...
Breaking Biology Technology:Rancho BioSciences, in Partnership with Omicsoft, have Integrated Array Suite with tranSMART 2Rancho BioSciences, in Partnership with Omicsoft, have Integrated Array Suite with tranSMART 3Molecular Diagnostics Market - Global Industry Size, Share, Trends, Analysis, Players, Opportunity, Outlook and Forecast From 2014 to 2020 2Molecular Diagnostics Market - Global Industry Size, Share, Trends, Analysis, Players, Opportunity, Outlook and Forecast From 2014 to 2020 3Molecular Diagnostics Market - Global Industry Size, Share, Trends, Analysis, Players, Opportunity, Outlook and Forecast From 2014 to 2020 4Molecular Diagnostics Market - Global Industry Size, Share, Trends, Analysis, Players, Opportunity, Outlook and Forecast From 2014 to 2020 5The Evolution of RNA-Sequencing, Upcoming Live Webinar August 22 hosted by Xtalks 2
... PARK, N.C. and MIAMI, Oct. 13 The Alpha-1 ... grants to study how genetic variations in the alpha-1 ... in people with AAT deficiency. The inherited condition causes ... whether AAT infusion therapy -- known as augmentation therapy ...
... , CHICAGO, Oct. 13 Advanced Life Sciences ... company engaged in the discovery, development and commercialization ... infection, cancer and respiratory diseases, today announced that ... held October 28-29th in San Francisco. , (Logo: ...
... LONDON, October 13 Systagenix Wound Management ... Board, consisting of,a group of multi-disciplinary and highly respected ... which will be led by,Systagenix,s newly appointed Medical Director, ... major specialist organisations and thought leaders in the,wound care ...
Cached Biology Technology:The Alpha-1 Foundation Awards Two Research Grants with Support from Talecris Biotherapeutics, Inc. 2The Alpha-1 Foundation Awards Two Research Grants with Support from Talecris Biotherapeutics, Inc. 3The Alpha-1 Foundation Awards Two Research Grants with Support from Talecris Biotherapeutics, Inc. 4Advanced Life Sciences to Present at 8th Annual BIO Investor Forum 2Systagenix Announces Formation of New Global Medical Advisory Board to Support Future Clinical Advances in Wound Care 2Systagenix Announces Formation of New Global Medical Advisory Board to Support Future Clinical Advances in Wound Care 3Systagenix Announces Formation of New Global Medical Advisory Board to Support Future Clinical Advances in Wound Care 4Systagenix Announces Formation of New Global Medical Advisory Board to Support Future Clinical Advances in Wound Care 5Systagenix Announces Formation of New Global Medical Advisory Board to Support Future Clinical Advances in Wound Care 6
(Date:7/31/2014)... SHELTON, Conn. , July 31, 2014 /PRNewswire/ ... on the growing m-commerce market, reminds investors and ... generation smart wallet, Wocket™, that it will be on-air ... biometrically secure Wocket smart wallet will be featured ... consumer oriented television show that airs on the ...
(Date:7/31/2014)... studies have shown that marine noise can affect animal ... the Universities of Bristol and Exeter and the cole ... demonstrated that boat noise stops embryonic development and increases ... the sea slug Stylocheilus striatus used in ... away and later feed on toxic alga but this ...
(Date:7/31/2014)... new meta-analyses involving tree nuts (almonds, Brazil nuts, cashews, ... recently published in the online publications, British Medical ... ONE (ii). The BMJ Open article ... syndrome (MetS) criteria and showed that tree nut consumption ... blood glucose. The PLOS ONE article focused ...
Breaking Biology News(10 mins):NXT-ID's Next Generation Biometric Smart Wallet Featured on NewsWatch Consumer Show on the History Channel and FYI Network Today, July 31st 2NXT-ID's Next Generation Biometric Smart Wallet Featured on NewsWatch Consumer Show on the History Channel and FYI Network Today, July 31st 3Boat noise impacts development and survival of sea hares 2New international tree nut council study looks at nuts, diabetes and metabolic syndrome 2
... Researchers studying a type of cell found in the trillions ... how it responds to brain injury and disease such as ... trigger the processes that underlie how astrocyte cells respond to ... humans, are a type of glial cell that comprise one ...
... 25, 2013 , , , WHEN:               ... 1, 2013 , , , , LOCATION:       ... , , , SPEAKER:        , , , ... Augmented reality (AR) is here to ...
... of regular aerial surveys and genetics data collected by a ... the central Gulf of Maine as a mating ground for ... online in the journal Endangered Species Research . ... one of the most endangered marine mammal species in the ...
Cached Biology News:Key target responsible for triggering detrimental effects in brain trauma identified 2The (Augmented) Reality of the Situation: Emerging Trends and Issues 2The (Augmented) Reality of the Situation: Emerging Trends and Issues 3Are North Atlantic right whales mating in the Gulf of Maine? 2Are North Atlantic right whales mating in the Gulf of Maine? 3
... PRODUCT SPECIFICATIONS Form: White powder Assay: 1.5 - 2.0 Bleomycin ... 6.0 Loss On Drying: less than or equal to 6.0% ... A 2 : 55 - 70% B 2 : 25 ... or equal to 90% B 4 : less than or ...
... High Vacuum pumps deliver critical performance for ... ballast control optimizes pump performance to suit ... allows for convenient checking of proper oil ... of pumps is designed to work with ...
6 x Blend of rTth DNA Polymerase and Tli to provide optimized amounts of both 5-3 and 3-5 proofreading activity...
This spacer is required to use the Miniature FRENCH Pressure Cell (FA-003) with the Laboratory Press....
Biology Products: