Navigation Links
Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation

Choose Shrimp Alkaline Phosphatase for Easier Heat Inactivation

Simplify dephosphorylation of 5′ ends on DNA vectors by using our high-quality Shrimp Alkaline Phosphatase. Avoid phenol:chloroform extractions; after dephosphorylation, inactivate Shrimp Alkaline Phosphatase completely and irreversibly by heating at 65 C for only 15 minutes.
Enjoy the convenience of a single-tube reaction; perform the restriction digestion, dephosphorylation, alkaline phosphatase inactivation, and ligation all in the same tube.

Ligate DNA in Five Minutes with the Rapid DNA Ligation Kit

Experience DNA ligation that is fast, flexible, and easy to perform with the original kit for rapid ligations.

  • Perform highly efficient ligations in 5 minutes at room temperature.
  • Ligate sticky- or blunt-end DNA fragments.
  • Generate constructs, transform competent cells, and plate bacteria all on the same day, letting you pick transformed colonies the next day.
  • Save time with a complete kit that contains enzymes and ready-to-use buffers.

Typical results: cloning of an insert into plasmid vectors

50 ng of pUC19 DNA, digested with Sma I or Hind III, respectively, and dephosphorylated with alkaline phosphatase, was ligated with 150 ng blunt- or sticky-end insert DNA, respectively, according to the standard protocol. The inserts were purified by electrophoresis, then isolated from the agarose gel by agarase digestion.


'"/>

Source:


Page: All 1

Related biology technology :

1. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
2. Genotyping Single Nucleotide Polymorphisms with Molecular Beacons
3. New Protocols for Isolating High- Molecular-Weight Genomic DNA
4. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
5. Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A
6. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
7. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
8. Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)
9. Near Infrared Technology and Optical Agents For Molecular Imaging
10. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
11. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/27/2016)... Diego, CA (PRWEB) , ... June 27, 2016 , ... ... solutions for clinical trials, announced today the Clinical Reach Virtual Patient Encounter ... their care circle with the physician and clinical trial team. , Using the CONSULT ...
(Date:6/27/2016)... 27, 2016  Liquid Biotech USA ... of a Sponsored Research Agreement with The University ... (CTCs) from cancer patients.  The funding will be ... correlate with clinical outcomes in cancer patients undergoing ... then be employed to support the design of ...
(Date:6/24/2016)... 24, 2016 Epic Sciences unveiled a ... susceptible to PARP inhibitors by targeting homologous recombination ... The new test has already been incorporated into ... cancer types. Over 230 clinical trials ... pathways, including PARP, ATM, ATR, DNA-PK and WEE-1. ...
(Date:6/23/2016)... ... June 23, 2016 , ... Mosio, a ... eBook, “Clinical Trials Patient Recruitment and Retention Tips.” Partnering with experienced clinical research ... by providing practical tips, tools, and strategies for clinical researchers. , “The landscape ...
Breaking Biology Technology:
(Date:5/12/2016)... WearablesResearch.com , a brand of Troubadour Research & ... Q1 wave of its quarterly wearables survey. A particular ... a program where they would receive discounts for sharing ... "We were surprised to see that so many ... CEO of Troubadour Research, "primarily because there are segments ...
(Date:4/26/2016)... and LONDON , April ... part of EdgeVerve Systems, a product subsidiary of ... today announced a partnership to integrate the Onegini ...      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ) ... their customers enhanced security to access and transact ...
(Date:4/14/2016)... Israel , April 14, 2016 ... Authentication and Malware Detection, today announced the appointment of ... assumed the new role. Goldwerger,s leadership appointment ... on the heels of the deployment of its platform ... BioCatch,s behavioral biometric technology, which discerns unique cognitive and ...
Breaking Biology News(10 mins):