Navigation Links
Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation

Choose Shrimp Alkaline Phosphatase for Easier Heat Inactivation

Simplify dephosphorylation of 5′ ends on DNA vectors by using our high-quality Shrimp Alkaline Phosphatase. Avoid phenol:chloroform extractions; after dephosphorylation, inactivate Shrimp Alkaline Phosphatase completely and irreversibly by heating at 65 C for only 15 minutes.
Enjoy the convenience of a single-tube reaction; perform the restriction digestion, dephosphorylation, alkaline phosphatase inactivation, and ligation all in the same tube.

Ligate DNA in Five Minutes with the Rapid DNA Ligation Kit

Experience DNA ligation that is fast, flexible, and easy to perform with the original kit for rapid ligations.

  • Perform highly efficient ligations in 5 minutes at room temperature.
  • Ligate sticky- or blunt-end DNA fragments.
  • Generate constructs, transform competent cells, and plate bacteria all on the same day, letting you pick transformed colonies the next day.
  • Save time with a complete kit that contains enzymes and ready-to-use buffers.

Typical results: cloning of an insert into plasmid vectors

50 ng of pUC19 DNA, digested with Sma I or Hind III, respectively, and dephosphorylated with alkaline phosphatase, was ligated with 150 ng blunt- or sticky-end insert DNA, respectively, according to the standard protocol. The inserts were purified by electrophoresis, then isolated from the agarose gel by agarase digestion.


'"/>

Source:


Page: All 1

Related biology technology :

1. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
2. Genotyping Single Nucleotide Polymorphisms with Molecular Beacons
3. New Protocols for Isolating High- Molecular-Weight Genomic DNA
4. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
5. Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A
6. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
7. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
8. Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)
9. Near Infrared Technology and Optical Agents For Molecular Imaging
10. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
11. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/21/2017)... ... April 21, 2017 , ... Having worked on the design ... is pleased to introduce it to top lab design architects from around the country ... and VP of Industrial Design and Engineering Greg Casey will be at the show, ...
(Date:4/21/2017)... (PRWEB) , ... April 21, 2017 , ... The AMA ... 11 high school graduates from across the nation. The scholarships are created through funds ... member dues. , Scholarship criteria are set by the AMA Scholarship Committee, which is ...
(Date:4/20/2017)... Ca (PRWEB) , ... April 20, 2017 , ... ... Virtual Event , this new webinar will explore challenging patient cases when screening ... to the hospital, there may be a need for bridging parental anticoagulation especially ...
(Date:4/20/2017)... ... , ... USDM Life Sciences , the leading risk management, technological innovation ... to announce Holger Braemer as Vice President of its Europe division and ... , Braemer is an integral part of USDM’s expansion of services and solutions ...
Breaking Biology Technology:
(Date:4/3/2017)... April 3, 2017  Data captured by ... platform, detected a statistically significant association between ... to treatment and objective response of cancer ... to predict whether cancer patients will respond ... as well as to improve both pre-infusion potency ...
(Date:3/30/2017)... Trends, opportunities and forecast in this market ... (fingerprint, AFIS, iris recognition, facial recognition, hand geometry, vein ... use industry (government and law enforcement, commercial and retail, ... others), and by region ( North America ... Pacific , and the Rest of the World) ...
(Date:3/27/2017)... March 27, 2017  Catholic Health Services (CHS) ... Systems Society (HIMSS) Analytics for achieving Stage 6 ... sm . In addition, CHS previously earned a ... using an electronic medical record (EMR). ... level of EMR usage in an outpatient setting.  ...
Breaking Biology News(10 mins):