Navigation Links
Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column

ning buffer consisted of 0.1M NaH2PO4 (pH 6.0) to which was added 15% acetonitrile and 0.2M NaCl. In the redesigned buffer system, NaCl was replaced by NaClO4 and the phosphate buffer concentration was reduced to 0.05M. Initially, the concentration of sodium perchlorate investigated was 0.2M, however, best results were obtained at 0.4M. The flow rate of 0.35mL/min was left unchanged. Note that recent work has shown that acetonitrile can be omitted from the mobile phase without affecting separation quality or method robustness.


Results:
As shown in Figure 1 , excessive peak tailing of "fusion protein 1" is evident with the use of 0.2M NaCl. Additionally, the expected protein dimer and trimer aggregates are not visible in the chromatogram. By switching from 0.2M sodium chloride to 0.2M of the more chaotropic sodium perchlorate salt, together with a two-fold reduction in the buffer concentration, less peak tailing and distinct peaks for the dimer and trimer species of MAb1 resulted. Doubling the perchlorate concentration to 0.4M provided further improvement in the peak shape of fusion protein 1 and associated aggregate species. Additionally, the re-development increased the methods' robustness allowing for a substantial decrease in column "break-in" time from two weeks to 4 days as well as better peak area reproducibility and linearity of both monomer and aggregate species.


As a precaution, a more rigorous cleaning protocol consisting of flushing the system with DI water was implemented during periods of instrument inactivity. Although perchlorate is slightly less corrosive to stainless steel components relative
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
2. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
3. Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel
4. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
5. Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System
6. Identification of Phase I and Phase II Metabolites of Buspirone on the Q TRAP LC/MS/MS System
7. Strategies to Identify and Confirm Phase I Metabolites of Glyburide Using the 4000 Q TRAP System
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
9. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
10. PCR Optimization
11. PCR Optimization Kit
Post Your Comments:
(Date:8/19/2014)... 2014 Robin Williams’ passing is a ... on an individual. Symptoms range from slowness of ... swallowing and severe depression. Parkinson’s disease progressively gets ... dopamine producing neurons of the brain. As these ... slowly and progressively gets worse, they look for options. ...
(Date:8/19/2014)... 2014 Research and Markets ... "Spectroscopy Equipment and Accessories - Global ... offering. This report analyzes ... Accessories in US$ Thousands by the following ... Spectrometers, NMR-EPR Spectrometers, Near Infrared (NIR) Spectrometers, ...
(Date:8/19/2014)... (PRWEB) August 19, 2014 In November 2012, ... Benefit Risk Evaluation Report (PBRER) in a new PSUR – ... post-authorization safety benefit-risk profile of a drug. Adopted in many ... , Present a comprehensive and critical analysis of ... , Highlight any new evidence of the potential ...
(Date:8/19/2014)... , Aug. 19, 2014 /PRNewswire/ - iCo Therapeutics Inc. ... today reported results of its Oral Amphotericin B ... reservoirs.  The study, conducted by ImmuneCarta®, the immune ... vitro effectiveness of Oral Amp B in ... in individuals despite intensive treatment with antiretroviral therapy. ...
Breaking Biology Technology:As We Mourn Robin Williams’ Passing, His Death Sheds Light on Patients Struggling with Parkinson’s Disease 2As We Mourn Robin Williams’ Passing, His Death Sheds Light on Patients Struggling with Parkinson’s Disease 3Global Spectroscopy Equipment and Accessories - Strategic Business Report 2014 2Top Five Best Practices for Developing Your Periodic Benefit Risk Evaluation Report (PBRER), New Webinar Hosted by Xtalks 2iCo Therapeutics Announces Positive Oral Amphotericin B Study Results 2iCo Therapeutics Announces Positive Oral Amphotericin B Study Results 3
... Wis. - So, it has come to this: ... wishing to vote in Wisconsin's Nov. 7 elections. , ,Or ... year claims and counter-claims swirling around the "wedge issue" of ... complete their doctoral degree by fall, here are a few ...
... Drawing on the immense popularity of the Fox Broadcasting ... , computer software designers are preparing to exploit a consumer ... Software , a producer of products for digital video, music, ... FremantleMedia Licensing Worldwide to develop two software products based ...
... reen Bay, Wis. - According to a ... cure all policy that will not only improve Wisconsin's business ... recovery. After all, this strategy of mutual cooperation and regional ... sleepy mountain town. , ,Wait a minute. Wasn't Denver ...
Cached Biology Technology:Fact and friction: Putting election-year stem cell claims under the microscope 2Fact and friction: Putting election-year stem cell claims under the microscope 3Fact and friction: Putting election-year stem cell claims under the microscope 4American Idol spurs music software 2Denver prospered because of "dreaded" TABOR 2Denver prospered because of "dreaded" TABOR 3
(Date:8/20/2014)...  The second annual Biometrics UnPlugged Executive Summit, ... Florida on September 15, 2014, will ... and Strategy at Samsung, and Philippe de Raet, Vice ... this year,s event is Mobility at the Crossroads ... UnPlugged: Mobility at the Crossroads of Commerce & ...
(Date:8/19/2014)... This news release is available in German . ... power are inherently intermittent energy sources. If a large amount ... in the future, excess energy will have to be stored ... for. However, existing storage capacities are far from adequate for ... better technologies. One important focus lies on battery systems that ...
(Date:8/19/2014)... from fish purchased at retail seafood counters in ... mislabeling can expose consumers to unexpectedly high levels ... "substitutions"which falsely present a fish of the same ... most dangerous mislabeling offense, according to new research ... "Accurate labeling of seafood is essential to allow ...
Breaking Biology News(10 mins):Biometrics UnPlugged: Mobility at the Crossroads of Commerce & Privacy to Feature Interactive Sessions with Samsung and Experian 2Asian inventions dominate energy storage systems 2Asian inventions dominate energy storage systems 3Seafood substitutions can expose consumers to unexpectedly high mercury 2Seafood substitutions can expose consumers to unexpectedly high mercury 3
... Chicago, November 23, 2010 Results of the world,s first ... or PrEP, were released online in the New England ... iPrEx, indicated an estimated 43.8% reduction of new HIV infections ... HIV, compared to those who took a placebo pill. ...
... how biological clocks adjust to having less sunlight in the winter ... shift work. Scientists studying the daily activity cycle in plants ... process that enables the plant,s genes to respond to the times ... of daylight in between. This system helps the plant to ...
... better tool to study how cells adhere to each other. ... stick together. Different kinds of cells have different kinds of ... focus on tens of thousands of them at a time ... but not on a single molecule. Sivasankar, an Iowa State ...
Cached Biology News:AIDS drug shown to prevent HIV in multinational trial of HIV-negative gay men 2AIDS drug shown to prevent HIV in multinational trial of HIV-negative gay men 3AIDS drug shown to prevent HIV in multinational trial of HIV-negative gay men 4Iowa State, Ames Lab researcher develops new way to study single biological molecules 2Iowa State, Ames Lab researcher develops new way to study single biological molecules 3
The Piezo Power MicroDissection (PPMD) system is designed for rapid isolation of single cells or small cell areas...
AC input: 50-60 Hz230 V...
AC input: 50-60 Hz 115-120 V...
... This reagent is formulated specifically for ... consistently high levels of activity for both ... storage at final dilution. This product ... stability providing the customer a longer shelf ...
Biology Products: