Navigation Links
Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System

Abstract

Immunoassays are valuable and widely used tools in the biological sciences. In drug discovery research, immunoassays are used to measure secreted cytokines in the tissue culture supernatants of treated cells. However, scientists often avoid immunoassays in high throughput screening (HTS) because they require cumbersome manipulations. Applied Biosystems has developed a singlestep, mix-and-read bead-based immunoassay, termed FLISA (fluorescence- linked immunosorbent assay), for the FMAT 8100 HTS System. The instrument is a macro-confocal scanner that enables mix-and-read, bead- and live cell-based HTS assays in 96- and 384-well formats. Furthermore, multiplexed assays, based on color or bead size, can be performed in a single well.

The unique features of the FMAT system allow bead-based FLISAs to be performed in the same well in which tissue culture cells are grown. A simple multiplexed protocol can simultaneously quantitate secreted cytokines and detect the upregulation of a cell surface marker on human umbilical vein endothelial cells (HUVEC) in a mix-and-read assay. After induction with IL-1 or TNF-, the cells are either stained with CentriRed Stain, a nucleic acid staining dye from Applied Biosystems, or with an anti-ICAM-1 antibody to measure upregulation of the cell surface adhesion molecule. At the same time, reagents used in the FLISA are added for quantification of secreted IL-8. Using the FMAT system for simultaneous measurement of secreted metabolites and cell surface markers simplifies data collection and enhances information obtained from a high throughput screen.

Introduction

Cytokines are important mediators in the inflammatory response of vascular endothelial cells (EC). Their presence promotes the secretion of additional pro-inflammatory cytokines and expression of cell-surface adhesion molecules. Upon activation with I
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
2. A Mix-and-Read Cell-Based Assay for Hybridoma Screening Using the FMAT 8100 HTS System
3. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
4. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
5. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
6. Oil-Free PCR Using the Hot Top Assembly
7. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
8. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
9. Using XL1-Red Mutator Strain to Generate Esterase Variants
10. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
11. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Mix and Read Bead and Cell Based Immunoassays Using the FMAT HTS System

(Date:1/15/2014)... 15, 2014   Niagara Thermal Products is proud ... BioBlower Technologies LLC ("B3") as the critical thermal component ... from the Army Corps of Engineers.  B3 has developed ... biological contaminants and destroys VOCs and other chemical pollutants, ...
(Date:1/15/2014)... Toronto, Canada (PRWEB) January 15, 2014 ... perspectives on nonclinical and clinical safety assessment in biosimilars. ... a path for biosimilar drug development, however the complex ... regards to quality, safety and efficacy extremely challenging. Based ...
(Date:1/15/2014)... 15, 2014 DTS Language Services, Inc ... Web Portal for Life Science organizations who need document translations. ... subject matter of their documents in advance with a selection ... time-to-delivery of translations, often a critical factor in clinical and ...
(Date:1/14/2014)... Date: Friday, April 11, 2014 , Time: 6 p.m. – ... Warrington, Pa. , Details: The Hepatitis B Foundation, ... cure for hepatitis B and improving the quality of life ... on Friday, April 11 at Warrington Country Club in Warrington, ...
Breaking Biology Technology:Niagara Thermal Products LLC Selected as Critical Supplier for Buffalo BioBlower Air Purification Systems 2Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2DTS Improves Efficiency for Life Science Document Translations 2Hepatitis B Foundation to Host Annual Crystal Ball Gala 2
... Nets to Prevent Serious Health Hazards ... Associated with Diethylene ... Pharmacopeial (USP),Convention today announced a revised Glycerin monograph in ... United States. The,revision strengthens safety nets to prevent serious ...
... The following was issued,today by the Greater Baltimore ... CEO, Greater Baltimore Committee, 140 company owners, executives ... The third annual Greater Baltimore Region Bioscience Awards, ... / Breakfast & Registration, 8:45 - 9:30 a.m. ...
... March 17 Mercury Therapeutics,Inc. (MTI), a ... protein,kinase targeted drugs, announces that it has ... advise on its strategic and partnering,opportunities, with ... shareholders., This appointment follows a significant ...
Cached Biology Technology:USP Announces Revised Glycerin Monograph 2USP Announces Revised Glycerin Monograph 3Greater Baltimore Committee to Announce Winners of Third Baltimore Region Bioscience Awards at Ceremonies on March 18 2Mercury Therapeutics, Inc: Marquant Appointed to Advise on Strategic and Partnering Opportunities 2Mercury Therapeutics, Inc: Marquant Appointed to Advise on Strategic and Partnering Opportunities 3
(Date:7/9/2014)... of a sparrow-sized fossil from China challenges the commonly ... that gained the ability to fly. The birdlike fossil ... much rather the remains of a tiny tree-climbing animal ... the Dinosaur Museum in Blanding, Utah, and Alan Feduccia ... in Springer,s Journal of Ornithology . , The ...
(Date:7/9/2014)... results accrue. To completely and systematically archive this ... fact, researchers in the life sciences spend a ... an online survey of 70 people working in ... Applied Information Technology FIT in Sankt Augustin. Many ... centralized or structured approach to data collection in ...
(Date:7/9/2014)... in their blood are more likely to survive the disease, ... of vitamin D have half the risk of dying compared ... The study is the first to correlate total blood levels ... which includes that produced after exposure to sunlight and ... survival prospects. , The University of Edinburgh team tested ...
Breaking Biology News(10 mins):Researchers declassify dinosaurs as being the great-great-grandparents of birds 2Managing the data jungle 2Managing the data jungle 3
... addictive substance in cigarette smoke, contributes to smokers, higher ... attacks, according to research to be presented Sunday, Dec. ... Meeting in New Orleans. These findings suggest ... steam without the carcinogenic agents of tobacco smoke, may ...
... An experimental drug that targets macrophages, a type of ... tumor glioblastoma multiforme decreased the cancer,s growth and extended ... report on Tuesday Dec. 17, at the American Society ... The rates of apoptosis, or programmed cell death, ...
... A cross-sectional study by investigators from Tokyo University has ... factor against sarcopenia and effective in maintaining muscle strength ... the ageing process, resulting in loss of skeletal muscle ... The multiple adverse health outcomes include physical disability, poor ...
Cached Biology News:Nicotine drives cell invasion that contributes to plaque formation in coronary arteries 2Blocking tumor-associated macrophages decreased glioblastoma's growth & extended survival in mice 2
... UltraPure Agarose and SYBR Safe DNA Gel Stain ... Saves money versus buying these same products separately ... alternative to ethidium bromide ... Agarose resolves DNA and RNA fragments from 100 bp ...
Anti-WT-1 Liquid. In 10 μM PBS, 0.2 % BSA, pH 7.6. Recognizes the ~47-55 kDa Wilm's tumor protein in Wilm's tumor tissue. ...
... uses continuous chemical suppression and thermally stabilized ... accurate determinations of common anions and cations ... quick to equilibrate, and fully automated with ... you achieve maximum sample throughput at minimum ...
... siRNA applications, contains magnetic nanoparticles (MagTag) which ... of interest. Exploiting magnetic force the full ... towards and delivered into the target cells ... be used for adherent cells; for suspension ...
Biology Products: