Navigation Links
Minimizing Photobleaching of Blue Fluorescent Protein (BFP) using ,,, the Varian Cary Eclipse Fluorescence Spectrophotometer

Paul Gavin*, Mark Prescott*, Ph.D, Daren J. Fyfe#, Ph.D and Jeffrey J. Comerford#, Ph.D

* Department of Biochemistry and Molecular Biology,
Monash University,
Clayton campus,
Victoria 3800, Australia

# Technical Assistance,
Varian Australia,
679 Springvale Road,
Mulgrave,
Victoria 3170, Australia
E-mail: fluorescence@varianinc.com

Introduction

The term photobleaching refers to loss of fluorescence emission from samples due to prolonged exposure to exciting radiation. While many fluorophores retain stable fluorescence after extended periods of illumination, some photobleach after short periods of time.

In order to maintain optimal fluorescence emission, it is important to minimize photobleaching. Strategies to achieve this usually involve limiting one or both of the following: (1) the time of exposure to, or (2) the intensity of, the exciting light. However, either of these strategies may compromise the quality of the results or limit the types of analyses that can be performed because the signal to noise ratio (S/N) is unavoidably decreased. Furthermore, kinetics-based assays performed over an extended period of time may not be possible due to increased exposure of the fluorophore to the excitation light which results in photobleaching.

Blue Fluorescent Protein (BFP) provides an example of a fluorophore that is susceptible to photobleaching: even with folding improvements, BFP still suffers from a relatively low fluorescence quantum yield [compared to green fluorescent protein] and relatively easy bleaching. (Tsien, 1998)1. Despite the use
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Automated Fluorescent-Tag Cycle Sequencing
2. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
3. Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit
4. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
5. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
6. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
7. Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers
8. Fluorescent pigments in living coral
9. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
10. A New C-Terminal GST Vector for Protein Production in S. pombe
11. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
Post Your Comments:
(Date:5/6/2015)... , May 6, 2015  Endo International plc (NASDAQ: ... De Silva , President and CEO, will present a corporate ... New York City on Wednesday, May 20, 2015 ... and audio archive for the event will be available on ... Relations, and then the link to the event. Participants should ...
(Date:5/6/2015)... 6, 2015  Oxis Biotech, Inc. (OXIS), a wholly ... announced today that Cassian Yee , M.D., Professor, ... at the University of Texas MD Anderson Cancer Center ... Professor, Department of Immunology, Division of Cancer Medicine, and ... Research at the University of Texas MD Anderson Cancer ...
(Date:5/6/2015)... The Remedy Group , the premier ... specialty pharmacy industry, will be exhibiting, presenting, and is ... Summit. The Summit will be held May 4-8, 2015 ... the largest annual gathering for the specialty pharmacy industry. ... providers, pharma/biotech manufacturers, and payers, to learn more about ...
(Date:5/5/2015)...  23andMe, Inc., the leading personal genetics company, today ... in collaboration with Pfizer Inc. The companies aim ... commonly known as lupus, into the study to help ... also in collaboration with the Lupus Research Institute, and ... Approximately 1.5 million people in the U.S. suffer ...
Breaking Biology Technology:Oxis Biotech Appoints Cassian Yee, M.D. To Its Scientific Advisory Board 2Oxis Biotech Appoints Cassian Yee, M.D. To Its Scientific Advisory Board 3The Remedy Group presenting at the 2015 Armada Specialty Pharmacy Summit: The Pitfalls to Growth and Maintaining the Patient Experience 223andMe Launches the Lupus Research Study in Collaboration with Pfizer Inc. 223andMe Launches the Lupus Research Study in Collaboration with Pfizer Inc. 3
... populations with regard to risk associated in the development of ... osteoporosis or cardiovascular disease, - North American ... in response to nutritional products (nutrigenomics),- North ... Feb. 28 Interleukin Genetics,Inc. (Amex: ILI ) reported today ...
... the following Webcast:, What: Transgenomic Inc. Fourth ... Conference Call, When: Thursday, February 28, ... How: Live over the Internet -- Simply ... address above., To access the call via telephone, ...
... SAN DIEGO, Feb. 28 Anadys Pharmaceuticals,Inc. (Nasdaq: ... in a,Phase I study of ANA773, the company,s oral ... first-in-human trial is a safety,and tolerability study designed to ... as to select the dose and,schedule for Phase II ...
Cached Biology Technology:Interleukin Genetics Enters into New Clinical Research Collaboration with Alticor 2Interleukin Genetics Enters into New Clinical Research Collaboration with Alticor 3Interleukin Genetics Enters into New Clinical Research Collaboration with Alticor 4Interleukin Genetics Enters into New Clinical Research Collaboration with Alticor 5Anadys Pharmaceuticals Commences Phase I Clinical Trial of ANA773 in Cancer Patients 2Anadys Pharmaceuticals Commences Phase I Clinical Trial of ANA773 in Cancer Patients 3Anadys Pharmaceuticals Commences Phase I Clinical Trial of ANA773 in Cancer Patients 4
(Date:4/20/2015)... -- Huntington Memorial Hospital is the first facility in ... wireless monitoring sensor to manage heart failure (HF). The ... heart failure monitoring device that has been proven to ... manage heart failure. The CardioMEMS HF System ... artery (PA) during a non-surgical procedure to directly measure ...
(Date:4/14/2015)... , April 14, 2015 NXT-ID, Inc. ... biometric authentication company focused on the growing mobile commerce ... nominated for the 2015 New York Design Awards under ... Design100, New York City Design Awards are part of ... on over 2,500 ratings from the marketplace, industry and ...
(Date:4/9/2015)... , April 9, 2015 Synaptics Inc. (NASDAQ: ... solutions, announced today that it will report financial results ... April 23, 2015, after the close of market. The ... and investors at 2:00 p.m. PT (5:00 p.m. ET), ... To participate on the live call, analysts and ...
Breaking Biology News(10 mins):Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 2NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 3Synaptics to Report Third Quarter Results on April 23 2
... Biology, researchers report using DNA from tissues samples collected in 1955 ... ( Orcinus orca ). In 1955, a pod ... a skeleton was saved in a museum in Wellington. Photographs were ... form of killer whale, characterized by a very small white eye-patch ...
... A decade-long JDRF-funded study led by the Institute ... providing a deeper understanding of the link between autoantibodies ... highlighting the importance of pre-diabetes research into possible preventions ... Autoantibodies and Risk of Progression to Diabetes in Children," ...
... 19, 2013  New York College of Health Professions announces the ... Office for detecting biometric changes through smart phones, apps and ... or killed. This patent is part of an ... College of Health Professions. Lisa Pamintuan , ...
Cached Biology News:New data on islet autoantibodies in young children defines early type 1 diabetes development 2New Technology Using Biometrics Detects If Security Guards Are Attacked, Injured Or Killed 2New Technology Using Biometrics Detects If Security Guards Are Attacked, Injured Or Killed 3New Technology Using Biometrics Detects If Security Guards Are Attacked, Injured Or Killed 4