Navigation Links
Microgenomic Expression Profiling

spectrophotometric readings. This novel formofmicro-spectrophotometry,inconcert with exisitng technologies commonly used for RNA quality assessment, allows for unprecedented process control during microgenomic experiments. The methods described herein are intended as general guidelines for microarray probe synthesis from limited amounts of RNA (≤2000 cells). Following laser microdissection of the desired cell population, RNA is isolated and then linearly amplified to generate antisense RNA (aRNA). When the starting cell population is very limited, a second round of linear amplification if necessary in order to have sufficient quantities of aRNA to use for probe synthesis. In the second round of amplification, complimentary DNA (cDNA)isgeneratedthroughfirstandsecond strand synthesis, and then used as template for amino-allyl dUTP incorporation during a second round of RNA amplification in preparation for fluorescent dye labeling. aRNA quality is typically assessed at critical steps in the workflow using the Agilent bioanalyzer system. Final probe quantity, as well as efficiency of dye incorporation, is determined using the NanoDrop ND1000 spectrophotometer in preparation for microarray analysis.

Instrumentation and Kits


Methods
Special Considerations:
◊ Minimize degradation by storing RNA at -70C.

◊ Frequent freeze-thaw cycles accelerate RNA degradation.

◊ RNase contamination will cause experimental failure. Adhere to the following recommendations to minimize RNase contamination:
Wear disposable gloves and change them frequently.
Clean work surfaces, instruments, racks, and reagent bottles with com mercially available RNase and DN asedecontaminationsolutionsbefore performing reactions.
After putting on
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
3. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
4. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
5. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
6. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
7. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
8. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
9. A New Lambda Vector for Mammalian Expression
10. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
11. Codon Bias-Adjusted BL21 Derivatives for Protein Expression
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Microgenomic Expression Profiling

(Date:11/22/2014)... Frederick, MD (PRWEB) November 21, 2014 ... sample management solutions provider, has released Limfinity® version ... more installation projects of Limfinity® than ever in ... majority configuration and minority framework enhancement. Limfinity® version ... goes far above and beyond bug fixes! , ...
(Date:11/22/2014)... , November 21, 2014   ... als Chief Commercial Officer zu KLOX    ... Unternehmensentwicklung und Chief Financial Officer bestellt    ... der Vermarktung seines jüngst zugelassenen Behandlungssystems zur Wundheilung zu ... bzw. das "Unternehmen") ist sehr erfreut, die folgenden personellen ...
(Date:11/21/2014)... (PRWEB) November 20, 2014 Author Matthew ... Walk to the Other Side” published as a ... Finalist in the “Fiction: Short Story” category of the ... Keen, President and CEO of USA Book News, said ... and independent publishers, including Simon & Schuster, Penguin, John ...
(Date:11/21/2014)... CA (PRWEB) November 21, 2014 Why ... that makes Albert Einstein so instantly recognizable? Why have ... physicist, mathematician, astronomer and author Hilton Ratcliffe seeks out ... have nothing at all to do with science. In ... Harbor Publishing , November 21, 2014), Ratcliffe puts it ...
Breaking Biology Technology:RURO Announces Limfinity® version 5.1.4 2KLOX Technologies meldet Neubesetzung von Führungspositionen 2KLOX Technologies meldet Neubesetzung von Führungspositionen 3KLOX Technologies meldet Neubesetzung von Führungspositionen 4KLOX Technologies meldet Neubesetzung von Führungspositionen 5Mystic Ink 's Short Story Collection “A Short Walk to the Other Side” Honoring Ray Bradbury Praised As Award-Winning Finalist In USA Best Book Awards 2Mystic Ink 's Short Story Collection “A Short Walk to the Other Side” Honoring Ray Bradbury Praised As Award-Winning Finalist In USA Best Book Awards 3Mystic Ink 's Short Story Collection “A Short Walk to the Other Side” Honoring Ray Bradbury Praised As Award-Winning Finalist In USA Best Book Awards 4“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 2“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 3“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 4
... Mass., Aug. 6 Repligen Corporation (Nasdaq: RGEN ) ... June 30, 2009. Total revenue for the quarter was $5,061,000 ... fiscal year 2009. The prior year results were favorably impacted ... royalties on the U.S. sales of Orencia(R) prior to the April ...
... RICHLAND, Wash. -- To use hydrogen as a clean energy ... molecule, rather than compressing the gas into a tank. A ... out of a molecule requires a catalyst. Now, researchers reveal ... step toward designing catalysts for use in hydrogen energy applications ...
... Exciton Technologies Inc. today announced that the Company ... and Drug Administration (FDA) to market exSALT(TM) SD7 ... coating technology utilizing silver oxysalts as the active ... effort and a strong belief in our product," ...
Cached Biology Technology:Repligen Reports First Quarter Fiscal Year 2010 Financial Results 2Repligen Reports First Quarter Fiscal Year 2010 Financial Results 3Repligen Reports First Quarter Fiscal Year 2010 Financial Results 4Repligen Reports First Quarter Fiscal Year 2010 Financial Results 5Repligen Reports First Quarter Fiscal Year 2010 Financial Results 6Repligen Reports First Quarter Fiscal Year 2010 Financial Results 7New clues about a hydrogen fuel catalyst 2New clues about a hydrogen fuel catalyst 3Exciton Technologies receives FDA approval to market exSALT(TM) 2Exciton Technologies receives FDA approval to market exSALT(TM) 3
(Date:11/18/2014)... , Nov. 17, 2014 The Parenteral Drug ... U.S. regulatory agencies will speak and at least seven more ... Conference at the Omni Shoreham Hotel in Washington ... once again to have significant support from the regulatory agencies ... Europe in our effort to help advance ...
(Date:11/11/2014)... Nov. 11, 2014  Forensicon, Inc., a ... pleased to announce the promotion of Yaniv Schiff ... Digital Forensics. In Schiff,s new role as Director, he ... of digital forensics examiners and provide leadership within the ... - http://photos.prnewswire.com/prnh/20141110/157719 Schiff joined ...
(Date:11/7/2014)... , November 6, 2014 Leading ... for better mobile security revolutionizing online transactions.  Companies in focus ... Holdings Ltd. (NYSE: BABA ), Google Inc. (NASDAQ: ... ), eBay Inc. (NASDAQ: EBAY ) and MasterCard ... NXTD and NXTDW), a biometric authentication company focused on ...
Breaking Biology News(10 mins):FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2Yaniv Schiff Promoted to Director of Digital Forensics at Forensicon, Inc. 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 3Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 4Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 5Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 6
... wrong place to protect vulnerable coral reefs from the effects ... Now the team led jointly by Newcastle University and ... action is needed to prevent the collapse of this important ... PLoS ONE , is the largest study of its ...
... science" breakthrough by Queen,s University researchers into regulating a ... will help prevent heart attacks and strokes. ... the study focuses on the effects of Viagra ... to treat pulmonary hypertension. The team,s findings will ...
... in the Olympics, but scientists have raced cane toads in ... to invade Melbourne, Adelaide or Hobart and are unlikely to ... According to research by Dr Michael Kearney, from the ... from Australia and the USA, the cane toad,s march will ...
Cached Biology News:No-take zones offer no boost for bleached reefs 2Study points to potential new use for Viagra 2Racing cane toads reveals they get cold feet on Southern Australia invasion 2Racing cane toads reveals they get cold feet on Southern Australia invasion 3
... Mouse polyclonal antibody raised ... Immunogen: ... 646 a.a) partial recombinant protein ... Accession Number: NM_006842 ...
Mouse monoclonal antibody to UGT2B10 - UDP glucuronosyltransferase 2 family, polypeptide B10...
... kit is a sample preparation kit for ... removal of detergents, phenolic compounds, salts, and ... dilute samples. The kit procedure involves a ... tissue or cells, plant leaves, E. coli, ...
... Factor XIII in both reduced and non-reduced ... XIII B-chain or human Factor XII. Factor XIII ... composed of two subunits. Factor XIII-A is the ... 160kDa. Factor XIII is present in plasma as ...
Biology Products: