Navigation Links
Microfluidic analysis of multiplex,,,PCR products for the genotyping of,,,Helicobacter pylori

and associated with increased mucosal interleukin 8 concentrations4.
In our studies5, we have found that certain H. pylori subtype combinations of the vacA, cagA and iceA genes possess a differentiating and predictive value for the development of gastric adenocarcinoma and MALT lymphoma. We have established a multiplex PCR analysis to detect and distinguish the different H. pylori genes and their allelic variants from DNA extracted from paraffin wax embedded tissues. The use of template DNA derived from formalin fixed and paraffin wax embedded tissue for the mPCR restricts the size of the PCR fragments to a maximum of about 300 bp due to fragmentation and poor DNA quality from such tissue. The primer sets used in the mPCR for the detection of the different H. pylori virulence genes generated amplification products with a maximum length of 301 bp. The combined use of primer sets in the mPCR was possible because each primer set required an annealing temperature of 58 C and all PCR products were distinguishable by size on slab gel electrophoresis. In order to obtain more reproducible and standardized results, a microfluidic based analytical platform, the Agilent 2100 bioanalyzer, using lab-on-a-chip technology6 was tested to replace slab gels as the tool for the electrophoretic separation of the mPCR products.
Material and methods
Tissues
The paraffin embedded and formalin fixed gastric tissues from chronic gastritis, gastric adenocarcinoma and gastric MALT lymphoma specimen were recruited from the archives of the Institutes for Pathology of the University of Mainz (Germany) and Cologne (Germany). Three 5-7 m-sections of each specimen were de-waxed in xylene and the nucleic acids were extracted by phenol/chloroform followed by precipitation in 300 mM sodium acetat
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. High-throughput gene expression analysis to screen for anti-asthma drugs
2. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
3. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
4. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
5. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
6. High resolution DNA analysis with the DNA 500 and DNA 1000 LabChip kits
7. Extended data analysis using DeCyder EDA
8. 2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
9. Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox
10. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
11. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
Post Your Comments:
(Date:9/17/2014)... , Sept. 17, 2014  MiMedx Group, Inc. ... processor and marketer of patent protected regenerative biomaterials ... today the publication of another peer-reviewed clinical study.    ... A Multi-center Randomized Controlled Clinical Trial Evaluating the ... Membrane Allografts and Multi-layer Compression Therapy vs. Multi-layer ...
(Date:9/17/2014)... PA & Rockville, MD (PRWEB) September 17, 2014 ... won a Small Business Innovation Research (SBIR) grant to ... immunotherapy. The award will provide ITI with approximately $255,000 ... Tree nuts, a leading cause of food allergy, affect ... cause of anaphylaxis behind peanut allergy. Tree nut exposure ...
(Date:9/17/2014)... 17, 2014 Alternative Fuels Americas, Inc. (AFAI) announced ... Special Purpose Vehicle in partnership with Jatro Greentech, Inc., a ... company. The SPV shall be called AFAI Jatro Green Ethiopia ... majority shareholder. The SPV shall pursue Jatropha planting and biofuels ... Ethiopia . "Although we have ...
(Date:9/17/2014)... de septiembre de 2014  Cryoport, Inc. ... mundial de soluciones logísticas avanzadas criogénicas para ... vida, anunció hoy la contratación de Broekman ... sede en Rótterdam, Países Bajos, para proporcionar ... en rápido crecimiento. Según los términos del ...
Breaking Biology Technology:MiMedx Multi-Center Randomized, Controlled VLU Clinical Study Published In Peer-Reviewed Journal 2MiMedx Multi-Center Randomized, Controlled VLU Clinical Study Published In Peer-Reviewed Journal 3MiMedx Multi-Center Randomized, Controlled VLU Clinical Study Published In Peer-Reviewed Journal 4Immunomic Therapeutics Awarded SBIR Grant to Develop Multi-Nut Allergy Immunotherapy 2Immunomic Therapeutics Awarded SBIR Grant to Develop Multi-Nut Allergy Immunotherapy 3AFAI Signs Joint Venture Agreement With Jatro Greentech Inc. 2AFAI Signs Joint Venture Agreement With Jatro Greentech Inc. 3Cryoport contrata a Broekman Group para mejorar su apoyo operativo y logístico en Europa 2Cryoport contrata a Broekman Group para mejorar su apoyo operativo y logístico en Europa 3Cryoport contrata a Broekman Group para mejorar su apoyo operativo y logístico en Europa 4Cryoport contrata a Broekman Group para mejorar su apoyo operativo y logístico en Europa 5
... CHARENTON-LE-PONT, France, January 10 Essilor,of America, Essilor ... in the United States with the acquisition,of Interstate ... independent,laboratories. Interstate,s two laboratories in Mansfield, Ohio and,Indianapolis, ... The,company has full-year sales of US$26 million and ...
... of,Maryland, Baltimore (UMB) BioPark today announced that Gliknik ... in the BioInnovation,Center, located in UMB BioPark,s Building ... for autoimmune diseases,and cancer, the foundation of which ... Professor and Chairman of the Department of,Otorhinolarynogology, University ...
... 9 Burrill Merchant Banking acted as,exclusive financial ... partnering of bilastine with the Menarini Group (Italy). ... bilastine rights in Europe,and other regions to Menarini ... is a new highly selective H1 antihistamine for ...
Cached Biology Technology:University of Maryland, Baltimore BioPark Signs Gliknik Inc. as First Tenant in Its BioInnovation Center in Building Two 2Burrill Merchant Banking Advises Faes Farma on Bilastine Licensing Agreement 2
(Date:9/17/2014)... research from the Copenhagen Centre for Social Evolution and ... theory that birth weight and -length can partially predict ... such as autism and schizophrenia later in life. The ... and subsequent hospital diagnoses for up to 30 years, ... The study is published today in the Proceedings ...
(Date:9/17/2014)... LANSING, Mich. Mutations in the human retinoblastoma protein ... Michigan State University scientists have turned to fruit fly ... gene. , In a paper featured on the ... of Biological Chemistry , Michigan State University researchers provide ... similar to those present in the human cancer gene, ...
(Date:9/17/2014)... growing significantly faster since the 1960s. The typical development ... they have accelerated -- by as much as 70 ... out by scientists from Technische Universitt Mnchen based on ... continuously observed since 1870. Their findings were published recently ... "forest dieback" was a hot topic, with the very ...
Breaking Biology News(10 mins):Size at birth affects risk of adolescent mental health disorders 2Size at birth affects risk of adolescent mental health disorders 3Abnormal properties of cancer protein revealed in fly eyes 2Global change: Trees continue to grow at a faster rate 2Global change: Trees continue to grow at a faster rate 3
... NY) As people age, their cells become less efficient ... buildup of toxic material that is especially pronounced in Alzheimer,s, ... the first time, scientists at the Albert Einstein College of ... an entire organ the liver and shown that, ...
... are polar opposite diseases, but both depend upon dopamine ... it; drug addicts get too much of it. Although ... well known, the way it works has been a ... of Medicine has revealed that dopamine strengthens and weakens ...
... a fly buzzing around the house. South Africans have an ... of Roridula gorgonias leaves. Attracted to the shiny ... soon trapped by the natural flypaper. However, this is not ... home to a population of Pameridea roridulae (mirid ...
Cached Biology News:In scientific first, Einstein researchers correct decline in organ function associated with old age 2In scientific first, Einstein researchers correct decline in organ function associated with old age 3Unlocking mystery of why dopamine freezes Parkinson's patients 2Unlocking mystery of why dopamine freezes Parkinson's patients 3How nonstick bugs evade natural fly paper 2
... I-96 Plate can be used either in ... large-scale (i.e., 96-well) purification of DNA and/or ... unique silica-based matrix make it perfect for ... RNA in = 10 l eluate per ...
AdestaTM Tissue/Cell Preservation Medium Sterile / Non-sterile: Sterility Tested Endotoxin: ...
... is the first all-inclusive collection of key ... Expression System. Methods developed by the leaders ... step-by-step protocols. This book will guide you ... strain construction and analysis, to production of ...
Rad 51C Immunogen: His-tagged human Rad 51C, over-expressed in E. coli Storage: 4 C...
Biology Products: