Microfluidic analysis of multiplex,,,PCR products for the genotyping of,,,Helicobacter pylori
Gastric infection with Helicobacterpylori (H. pylori) is the majorcause of chronic active gastritisand is associated with the pathogenesisof peptic ulcer and gastriccarcinoma. Recent studies using amultiplex PCR (mPCR) basedapproach distinguished differentall
Gastric infection with Helicobacter
pylori (H. pylori) is the major
cause of chronic active gastritis
and is associated with the pathogenesis
of peptic ulcer and gastric
carcinoma. Recent studies using a
multiplex PCR (mPCR) based
approach distinguished different
allelic variants that are associated
with different stages of H. pylori
virulence. H. pylori genotyping on
gastric tissues, routinely
processed in diagnostic pathology,
revealed that certain combinations
of virulence subtypes of different
H. pylori sub-strains are
associated with gastric carcinoma
and MALT lymphoma. The use of
formalin fixed samples as the
source for template DNA for
mPCR restricts the size of the
PCR fragments to 300 bp, due to
poor DNA quality. For the analysis
of multiple bands of a mPCR
experiment, the data acquisition
from slab gels is often inconvenient
in terms of sizing, resolution,
and data accuracy. To
improve accuracy and reproducibility
of the measurements, the Agilent 2100 bioanalyzer using
Lab-on-a-chip technology was tested
to replace slab gels as the tool
for the electrophoretic separation
of the mPCR products. First analyses
revealed an improved fragment
resolution, sizing accuracy
and reproducibility. A mPCR reaction
covering five different alleles
that are involved in H. pylori
pathogenesis was analyzed and all
fragments were separated and
quantified. The analysis of a mixture
of all mPCR reactions including
all involved alleles showed the
separation of seven different PCR
fragments in the range of 102 to
301 bp, thereby expanding the
spectrum of prognostic or therapeutically
relevant information
used e.g. in H. pylori diagnostic.
The human pathogen Helicobacter
pylori (H. pylori) is associated
with the development of a variety
o
'"/>Source:
Page: All 1 2 3 4 5 6 Related biology technology :1.
High-throughput gene expression analysis to screen for anti-asthma drugs2.
Fully automated DNA purification and efficient multiplex PCR for analysis
of microsatellite loci3.
Flow cytometric analysis of human
primary cells using the Agilent 2100
bioanalyzer and on-chip staining4.
2100 expert software
Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer5.
Using the Agilent 2100 bioanalyzer for
quality control of protein samples prior to
MS-analysis6.
High resolution DNA analysis with the
DNA 500 and DNA 1000 LabChip kits7.
Extended data analysis using DeCyder EDA8.
2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ9.
Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox10.
Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR11.
TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
(Date:11/19/2009)... and recognized refractive and cataract surgery ex...t their fellow surgeons up-to-date on the latest d... surgery, and ophthalmology in general at the rece...AAO) annual meeting in San Francisco, California. ...id Huang, MD, Dr. Randy Epstein, Dr. William Tratt...
(Date:11/18/2009)...ndards and Technology (NIST) has demonstrated a re...and measuring elusive hazards such as concealed ex... pesticides distributed in soil by wind and rain. ...tional techniques for detecting traces of these ma...ng distinct electrically positive and negative end...
(Date:11/18/2009)...nalysis combined with Bio-Rad,s Biotechnology ...rovide state-of-the-art solutions to biotechnology...swire/ -- Vernier Software & Technology, a pro...and software and Bio-Rad Laboratories, Inc., a mul...ience research and clinical diagnostics products t...
(Date:11/18/2009)...n and SwitchGear Genomics announce a novel method ... Madison, WI, USA (PRWEB) Nov...ar Genomics announce a novel method for characteri...using the HaloCHIP™ System and high-throughp...elate DNA binding events to transcriptional activa...
Breaking Biology Technology:Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 2Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 3Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 4Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 5Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 6Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 7Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 8Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 9Prototype NIST method detects and measures elusive hazards 2Vernier and Bio-Rad to Offer Enhanced Solutions for Biotechnology Education 2Promega and SwitchGear Publish Findings from Combined Technologies that Correlate Binding Events and Transcriptional Activity 2...The Future Strategies of Cervical Cancer Preventio...cilitates Discussion on the Prevention of Cervical..., CHICAGO, Sept. 9 The Society of Gynecologic On...ntion Forum on September 12-13,2008 at the Hotel S...aders,will be in attendance to discuss the undefin...
...ETHLEHEM, Pa., Sept. 9 A seven and a half-year st...oom Laboratory of the Lehigh,Valley Hospital and H...or,"UVC" lights installed in the HVAC system had a...cy rates (CPR). In presenting the findings at the,...ctive Medicine (ASRM),Kathryn C. Worrilow, Ph.D. r...
...ybritech Alumni Launched the World,s 3rd Largest B...han 175 Local Companies, SAN DIEGO, Sept. 9 The ...th Anniversary Reunion for Hybritech employees wil...the headquarters of AMN,Healthcare in San Diego., ...ddy" biotech company,began operations in temporary...
Other Biology Technology:Society of Gynecologic Oncologists to Host Cervical Cancer Prevention Forum 2Society of Gynecologic Oncologists to Host Cervical Cancer Prevention Forum 3Germicidal UVC Lights Improve Clinical Pregnancy Rates for IVF Lab, New Study Finds 2San Diego's Original Biotech Company Celebrates 30th Anniversary With Reunion 2... drug seems very promising on paper; things are,of...omerase inhibitors,to treat cancer; they are suppo...nitely) aspect of cancer cells. Yet, something in ... them to inhibit completely,the telomerase. What t...lse? Hell no! Look out for whats stopping the drug...
... with E.coli, can lead to urinary tract infections... , “We found out that UTIs may be caused by inges...bRoy, director of Penn State’s Gastroenteric Disea...ed, she noted. , Senior author, Dr. Lee W. Riley,...oli strains isolated from patients with UTIs were ...
...tory earlier ;,progress in this area is made in th...eding the slow (and tedious) process of gene,cloni...c). ,Devices the size of a pager now have greater ...entire room. Similar advances are being,made in th...rsity of,Houston, now allowing researchers to inex...
Other Biology News:Combination therapy boosts effectiveness of telomere-directed cancer cell death 2Bacteria collection sheds light on urinary tract infections 2Inexpensive, mass-produced genes core of synthetic biology advances at UH 2Rat siTrio from B-Bridge International
Human siTrio from B-Bridge International
Rat siTrio from B-Bridge International
...convenience from the former kit #17295. However, t... but also produced some advantages as follows. At ...ugated antibody from lyophilized product to liquid... conjugate has improved so that the conjugate can ...
Biology Products: