Navigation Links
Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System

on.

The DGGE patterns from ground water with different hydrochemical characteristics are shown in Figure 2. Patterns from anoxic ground water (lane 1) are different from those obtained with oxic ground water (lane 2). Some bands were found in both samples, indicating bacterial populations that could adapt to both habitats.

In order to demonstrate temporal changes in microbial populations, DGGE patterns from surface water (river Ruhr) were recorded over a period of 6 months. Figure 3 shows changing patterns where certain species of DNA are found over a limited period of time, while others are found in all samples.

The complex DGGE patterns are further analyzed and compared by digitized gel imaging using the model GS-700 Imaging Densitometer and Multi Analyst software (Bio-Rad). DGGE patterns from surface and ground water reveal a specific and unique bacterial population depending on the hydrochemical properties. In addition DGGE patterns are used to demonstrate temporal and spatial variations in species composition. Sudden changes in DGGE patterns might indicate the introduction of harmful compounds (e.g. pesticides, heavy metals) to surface and ground water. Analysis by DGGE is also suitable for a subsequent species identification by sequencing individual bands.7


References

1. Amann, R. I., Ludwig, W. and Schleifer, K.-H., Microbiol. Rev., 59, 143-169 (1995).

2. Kuhlmann, B., Eschweiler, B., Kilb, B., Preu, G., Ziemann, E. and Schttler, U., Vom Wasser 89, 205-214 (1997).

3. Eschweiler, B., Kilb, B., Kuhlmann, B., Preu, G.
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
2. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
3. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
4. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
5. PCR Grade Water
6. Enjoy a Greater Sense of Security with PCR-Grade Water
7. Which Water to Use?
8. Analysis of Phenolic Estrogen Mimics in River Water by Negative Ion Electrospray LC/MS/MS
9. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
10. Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System
11. Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis
Post Your Comments:
(Date:1/23/2015)... Portal Solutions , a technology consulting firm that ... 365 and SharePoint platforms , today announces its inclusion in ... of the region’s fastest-growing mid-sized companies based on a combined ... be recognized a second year in a row by SmartCEO ...
(Date:1/22/2015)...  Transwestern | RBJ today announces the firm brokered a long-term ... a leading biopharmaceutical company, at Two Ledgemont Center in ... Richards , president, and Brian Cohen , senior vice ... building at 95 Hayden Ave. Photo - ...
(Date:1/22/2015)... San Diego, California (PRWEB) January 22, 2015 ... R Promotional Offer to their comprehensive portfolio of ... Offer allows customers to purchase a Centrifuge 5424/5424 R ... Pipette Bundle of either Eppendorf Research plus or Eppendorf ...
(Date:1/22/2015)... Diagenode, Inc., a leading global provider ... complete solutions for epigenetics research, recently launched a ... need for manual processing. The new ChIPettor System ... histones or transcription factors and a semi-automated pipette ...
Breaking Biology Technology:Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 2Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 3Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2
... April 7 Transgenomic, Inc. (OTC Bulletin Board: TBIO), ... completed a licensing option with the Dana-Farber Cancer Institute, ... Cold-PCR. This variation of the standard PCR technology ... was invented at Dana-Farber by Dr. Mike Makrigiorgos who ...
... WellGen, Inc. today announced that Dr. Roderick (Roddy) ... Executive Officer. Dr. Carter, 45, takes over ... Chief Executive Officer. Mr. Bertoldi will continue in ... Financial Officer of Amphion Innovations plc (LSE: AMP), a ...
... Inc. today announced that it has secured $38 million ... formation in 2004, the company has raised $92 million ... financing round, which consists of two tranches, was led ... pro rata participation of existing investors Apax Partners, Clarus ...
Cached Biology Technology:Transgenomic Completes Licensing Option With Dana-Farber Cancer Institute on Cold-PCR for Enrichment of DNA Mutations 2Transgenomic Completes Licensing Option With Dana-Farber Cancer Institute on Cold-PCR for Enrichment of DNA Mutations 3Transgenomic Completes Licensing Option With Dana-Farber Cancer Institute on Cold-PCR for Enrichment of DNA Mutations 4Dr. Roddy Carter Named President and Chief Executive Officer of WellGen, Inc. 2Dr. Roddy Carter Named President and Chief Executive Officer of WellGen, Inc. 3Aerovance Secures $38 Million in Equity Financing 2Aerovance Secures $38 Million in Equity Financing 3
(Date:1/22/2015)... Jan. 13, 2015  Today, FreeWavz ( www.FreeWavz.com ), ... crowdfunding campaign on Fundable, https://www.fundable.com/freewavz . FreeWavz ... production capacity to meet customer demand. ... http://photos.prnewswire.com/prnh/20150113/168790 Invented by ...
(Date:1/22/2015)... 15, 2015  BellBrook Labs, a leader in high ... launch of a TR-FRET (time resolved Forster resonance ... Assay, a high throughput screening assay for glycosyltransferases ... allow for sensitive detection of hundreds of human ...
(Date:1/22/2015)... Inc. (NASDAQ: NXTD and NXTDW) a biometric authentication company ... of the Wocket™ smart wallet at CES 2015 in Las ... one of the "11 Hot Products at CES" in a review published ... Far" by Newseveryday.com and "The top 10 gadgets from CES 2015" by ...
Breaking Biology News(10 mins):FreeWavz Launches on Fundable to Drive Speed-to-Market in 2015 2BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 3
... working in College of Life Science, Zhejiang University, has ... CpG island has a parental differentially methylated region (DMR). ... imprinting exists in lower vertebrates and genomic imprinting should ... mammals. The study is reported in volume 54 (Issue ...
... A team of University of Oregon biologists, using fruit ... specific cells, opening a new window on how gene ... While DNA (deoxyribonucleic acid) provides an identical genetic blueprint ... that turn protein molecules on and off in different ...
... is the latest American Chemical Society (ACS) Weekly PressPac ... from ACS, 34 peer-reviewed journals and Chemical & Engineering ... Society as the source for this information. ... melamine-adulterated food , Journal of Agricultural and Food ...
Cached Biology News:The evolutionary foundation of genomic imprinting in lower vertebrates 2New tool isolates RNA within specific cells 2American Chemical Society's Weekly PressPac -- May 13, 2009 2American Chemical Society's Weekly PressPac -- May 13, 2009 3American Chemical Society's Weekly PressPac -- May 13, 2009 4American Chemical Society's Weekly PressPac -- May 13, 2009 5American Chemical Society's Weekly PressPac -- May 13, 2009 6American Chemical Society's Weekly PressPac -- May 13, 2009 7American Chemical Society's Weekly PressPac -- May 13, 2009 8American Chemical Society's Weekly PressPac -- May 13, 2009 9American Chemical Society's Weekly PressPac -- May 13, 2009 10
... for monitoring acetylcholinesterase (AChE) activity. This is ... 5 min), non-radioactive and easy to use ... natural substrate, acetylcholine. A series of coupled ... AChE into a change in luminescence. Inhibitors ...
... Pulsed Dynamic Focusing MALDI (PDF-MALDI) source provides ... that do not need online separation. Paired ... LC/MS and Agilents powerful Spectrum Mill software, ... analysis of 2D gel-spot digests. It provides ...
Rabbit polyclonal to Vitronectin ( Abpromise for all tested applications). Antigen: Full length Vitronectin protein (Mouse). Entrez Gene ID: 7448 Swiss Protein ID: P04004...
... The Vibratome Roto-Shake Genie is ... platform. It performs five different ... rolling/rocking and rotating/rolling, as well as ... The precision-engineered moving portion of ...
Biology Products: