Navigation Links
Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System

Bernd Eschweiler and Beate Kilb, Institute for Water Research, Schwerte, Germany


Introduction

Bacterial diversity in environmental samples is usually determined by a characterization of isolated strains. A problem for the analysis and characterization of microbial communities is the inability to culture most of the bacteria species present in the sample. Therefore isolated bacteria may account for only a minor portion of the total bacterial diversity originally present in the sample. This problem is particularly severe in oligotrophic habitats like ground water, where approximately only 0.1 to 1% of the bacterial species are culturable.1 A new approach in microbial ecology is based on the analysis of bacterial genetic information without cultivation. This culture-independent approach has greatly enhanced the ability to assess bacterial diversity in ecosystems such as ground and surface water environments. After isolation of total bacterial DNA, variable regions of the 16S rRNA gene are amplified by PCR*. The similar sized PCR-products are separated by subsequent DGGE, and the resulting diversity pattern are analyzed and compared.2, 3


Materials and Methods

DNA from surface and ground water was isolated and purified as described earlier.2, 3 Two universal bacterial 16S rDNA primers were used to amplify a 527 bp fragment from total genomic DNA. PCR was performed in a total volume of 100 l containing 1x PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl2, 0.001% gelantine), 200 M each dNTP, 0.5 M each primer, 0.3 mg/ml bovine serum albumin (BSA), and 2.5 U AmpliTaq DNA polymerase (Perkin
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
2. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
3. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
4. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
5. PCR Grade Water
6. Enjoy a Greater Sense of Security with PCR-Grade Water
7. Which Water to Use?
8. Analysis of Phenolic Estrogen Mimics in River Water by Negative Ion Electrospray LC/MS/MS
9. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
10. Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System
11. Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis
Post Your Comments:
(Date:1/15/2014)... Freeslate, Inc ., the leading ... Lupin Limited, one of India’s top five pharmaceutical companies, ... System for high throughput solid form screening. ... a wide range of quality, affordable generic and branded ...
(Date:1/14/2014)... DC (PRWEB) January 14, 2014 Carahsoft ... for Thursday, January 23, 2014 at 2pm EST (11am ... Knowledge.” The topic focuses on how technology can turn ... critical decisions for government agencies. The online webinar will ...
(Date:1/14/2014)... York, NY (PRWEB) January 14, 2014 ... of Alliqua, Inc. (OTCQB: ALQA). Alliqua is an emerging ... to serve the wound care market. , Free report ... was restructured with a seasoned management team and Board, ...
(Date:1/14/2014)... 2014 In recent years, growing suspicion ... in product development and promotion has led to unprecedented ... fueled by concerns about the insidious impact of commercialization ... spectacular fines to the world’s biggest pharmas for illegal ...
Breaking Biology Technology:Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 3
... Palatin Technologies, Inc. (NYSE Alternext US: PTN) will announce ... Tuesday, February 17, 2009 before the open of the ... and live audio webcast hosted by its executive management ... of the results of operations in greater detail and ...
... Inc. ("OriGene") today announced it has raised an ... With this financing, OriGene completed the acquisition of ... a manufacturer and provider of monoclonal antibodies (mAb) ... completes the establishment of its technology center to ...
... CARLOS, Calif., Feb. 11 Nektar Therapeutics,(Nasdaq: ... fourth quarter and,year ended December 31, 2008 on Wednesday, ... Howard Robin, president and chief executive,officer, will host ... p.m. Eastern Time (ET)/2:00 p.m. Pacific Time (PT). , ...
Cached Biology Technology:Palatin Technologies to Report Fiscal Year 2009 Second Quarter Results; Conference Call and Webcast on February 17, 2009 2OriGene Technologies, Inc. Establishes High Throughput Monoclonal Antibody Production Outsourcing Center in China 2Nektar to Announce Year-End 2008 Financial Results on Wednesday, February 25, 2009 After Close of U.S.-Based Financial Markets 2
(Date:4/20/2014)... NEW YORK, NY (April 20, 2014) A ... (CUMC), Weill Cornell Medical College, and Brandeis University ... treatment of Alzheimer,s disease involving the so-called retromer ... neurons, steering amyloid precursor protein (APP) away from ... cleaved, creating the potentially toxic byproduct amyloid-beta, which ...
(Date:4/20/2014)... doctors have long known that people with Down syndrome ... (ALL) during childhood, they haven,t been able to explain ... investigators has uncovered a connection between the two conditions. ... journal Nature Genetics , the researchers track the ... in Down syndrome to the cellular havoc that occurs ...
(Date:4/18/2014)... 20 years, researchers have published soil organic carbon sequestration ... that soil organic carbon can be sequestered by simply ... systems. However, there is a growing body of research ... rotations without cover crops, small grains, and forages may ... published rates. , "Some studies have shown that both ...
Breaking Biology News(10 mins):'Chaperone' compounds offer new approach to Alzheimer's treatment 2'Chaperone' compounds offer new approach to Alzheimer's treatment 3Dana-Farber researchers uncover link between Down syndrome and leukemia 2Dana-Farber researchers uncover link between Down syndrome and leukemia 3Researchers question published no-till soil organic carbon sequestration rates 2Researchers question published no-till soil organic carbon sequestration rates 3Researchers question published no-till soil organic carbon sequestration rates 4
... rises outdoors, it,s a fitting time to consider the ... Complex interactions among temperature, water cycling, and plant ... to be answered as we face a rapidly changing ... California, Berkeley) recently tackled one aspect of the challenging ...
... A new system for the controlled delivery of pharmaceutical ... of Rhode Island chemical engineers using nanoparticles embedded in ... fields. The discovery by URI professors Geoffrey Bothun ... published in the June issue of ACS Nano. ...
... events could become commonplace in the United States in the ... University climate scientists. "Using a large suite ... much more intense, hot conditions in the U.S. within the ... environmental Earth system science at Stanford and the lead author ...
Cached Biology News:Ferns and fog on the forest floor 2Researchers develop drug delivery system using nanoparticles triggered by electromagnetic field 2Heat waves could be commonplace in the US by 2039, Stanford study finds 2Heat waves could be commonplace in the US by 2039, Stanford study finds 3Heat waves could be commonplace in the US by 2039, Stanford study finds 4
... Non-ionic Detergent Removal , , ... virtually no cross-reactivity with other proteins , ... , Economical new surface technology, not ... often interfere with protein analysis. SurfactAway offers ...
SDS-OutT SDS Precipitation Reagent...
... a platform for fully automated systems for ... easily be configured for applications ranging from ... allows closed loop screening, which means it ... generating, cherry picking and follow up studies ...
... The Staccato Series of systems provide fast and reliable ... and drug development laboratories. Staccato systems are available in ... Custom Systems Series. ... All ...
Biology Products: