Navigation Links
Microarray Target Purification Kit and,,,the Microarray Target Synthesis Kit (T7)


The Microarray Target Synthesis Kit (T7) has been developed as a robust, reliable and highly reproducible tool for the preparation of large amounts of labeled cRNA when starting with only 105 106 cells for target preparation. Major features are:
  • acceptance of low amounts of cDNA (obtained from 1 g total RNA)
  • short reaction time of only 2 3 hours (Figure 2)
  • high yields of cRNA in the range of 100 g
  • compatible to a broad spectrum of hapten or fluorophore-labeled nucleotides
  • cost-effective labeling due to optimized nucleotide concentrations and reaction conditions



Targets, synthesized and labeled with the Microarray Target Synthesis Kit (T7) have been tested on various commercially available DNA arrays. We have found that it is sufficient to use only one labeled nucleotide to get reliable results compared to double-labeling procedures in the majority of experiments.

Very often sample material is available in such small quantities (starting with less than 1,000 cells) that efficient target preparation requires PCR-based amplification methods. Within the Microarray Target Synthesis Kit (PCR), we have optimized a random primer-based PCR amplification procedure which allows the efficient and reproducible amplification of mRNA out of 50 ng total RNA.

The resulting PCR product can then be labeled by different methods, e.g., with the Microarray RNA Target Synthesis Kit (T7) to obtain single stranded cRNA.




'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. New Microarray Labeling Kit for Preparing cDNA Probes
2. Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
3. An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat
4. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
5. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
6. Increased Sensitivity in Microarray Analysis
7. Analysis of Microarray Data
8. Cancer-Related miRNAs Uncovered by the mirVana miRNA Microarray Platform
9. Is RNA Amplification Necessary for Microarrays?
10. Perform Microarray Analysis with Limited Bacterial RNA
11. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
Post Your Comments:
(Date:7/1/2015)... Mass. , July 1, 2015   ... most frequently cited advantages of Otezla are its ... safety profile, as reported by surveyed rheumatologists. Otezla ... launched for the treatment of active psoriatic arthritis ... The current standard of care includes conventional oral ...
(Date:7/1/2015)... 2015  Axovant Sciences Ltd. (NYSE:  AXON), a ... of dementia, today announced that the company will ... 5:45 p.m. EDT following its presentation of RVT-101 ... 2015 (AAIC). The event will ... Lawrence Friedhoff , Chief Development Officer, will ...
(Date:7/1/2015)... ... ... Apex Therapeutics announced today that company CEO, David Broecker provided a company update ... took place on Tuesday, June 16 and provided an update on the achievement of ... of pancreatic cancer. A copy of the company overview can be found on ...
(Date:6/30/2015)... ... 30, 2015 , ... Students and faculty from Beijing City ... of “Integrative Biotechnology,” an applied biotechnology course team-taught by faculty members from both ... students from both universities and is taught on both continents. , “Our alliance ...
Breaking Biology Technology:Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 2Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 3Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 2Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 3Apex Therapeutics Presented Company Overview at This Year’s BIO International Convention in Philadelphia, PA 2Rivier University and Beijing City University Academic and Cultural Exchanges Begin 2
(Date:6/30/2015)... June 30, 2015 Genisphere announced ... CEO to help further develop Genisphere,s therapeutics capabilities and ... experience, having spent much of the last 25 years ... Baxter Bioscience, and Johnson & Johnson. Tom started his ... and Biotech Corporate Finance. He graduated from Dartmouth College ...
(Date:6/25/2015)... , June 25, 2015 ... Market by Type (Swipe & Area), Technology, Material (Optical ... Healthcare, Commercial Security & Others) & Geography - ... marketsandmarkets, the said market is expected to reach ... 17.1%. Browse 76 market Tables ...
(Date:6/24/2015)... , June 24, 2015 The biologics ... The major drivers for the market include growth of ... of new drug launches. Over the years, the number ... Between 2001 and 2010, the FDA approved 225 drug ... to WIPO, Europe accounted for ...
Breaking Biology News(10 mins):Genisphere Appoints New CEO, Thomas Bliss 2Genisphere Appoints New CEO, Thomas Bliss 3Fingerprint Sensors Market Worth $14,500.07 Million by 2020 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 3Fingerprint Sensors Market Worth $14,500.07 Million by 2020 4Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 2Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 3Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 4
... 31, 2008 Staying awake slows down our brains, scientists ... sleep but inevitably trends downward throughout the day, and sleep ... time, researchers at Washington University School of Medicine in St. ... When scientists genetically tweaked a part of the brain involved ...
... and his students have developed a nanomaterial that ... result in more fuel-efficient airplanes and cars as ... The material xGnP Exfoliated Graphite NanoPlatelets ... new and expanded applications in the aerospace, automotive ...
... New Zealand - Kiwifruit lovers can look forward to ... the release this week of crucial genetic data which ... varieties with increased health properties and exciting colours and ... HortResearch and listed New Zealand biotech company Genesis Research ...
Cached Biology News:Brain tweak lets sleep-deprived flies stay sharp 2'Small' research at MSU leads to advances in energy, electronics 2Genetic data promises new future for kiwi fruit 2Genetic data promises new future for kiwi fruit 3
Phospho-mu-Opioid Receptor (Ser375) Antibody...
Rabbit polyclonal to WWOX8 ( Abpromise for all tested applications). entrezGeneID: 51741 SwissProtID: Q9NZC7-8...
... PhosphoSafe Extraction Reagent extracts cytosolic ... cells while preserving their phosphorylation ... of the CytoBuster Protein Extraction ... PhosphoSafe is compatible with Western ...
Goat polyclonal to T7 tag (Agarose) Immunogen: Goats were immunized with MASMTGGQQMG (T7) conjugated to KLH.After multiple immunizations in Freund's adjuvant, serum was collected....
Biology Products: