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Microarray Target Purification Kit and,,,the Microarray Target Synthesis Kit (T7)

workflow from RNA isolation to preparation of labeled targets. After each step, it is essential that residual impurities or nucleases are removed from the reaction products (single-stranded [ss] or double-stranded [ds] cDNA, cRNA or PCR products). Therefore, we have developed a nucleic acid purification kit that is universally applicable to the whole range of potential samples.

Product Description

cDNA Synthesis System

The well-established cDNA Synthesis System provides a convenient one-tube procedure for the synthesis of ds cDNA, starting even with small amounts of total RNA (120 g). Using the supplied Oligo ([dT24] T7 promoter) primer, cDNA is synthesized containing a T7 RNA polymerase promoter for the subsequent transcription reaction with T7 RNA polymerase.

Microarray Target Purification Kit

The Microarray Target Purification Kit is specifically designed for the purification of a variety of synthesized nucleic acids in the target labeling and amplification process, such as:
  • ss cDNA after 1st strand cDNA synthesis
  • ssDNA after cDNA labeling
  • ds cDNA after 2nd strand cDNA synthesis
  • PCR products
  • cRNA after in-vitro transcription

The kit will remove residual nucleotides, primers, proteins, nucleases and other reaction components which will otherwise interfere in the workflow for highly sensitive microarray hybridization. The process does not require nucleic acid precipitation, phenol/chloroform extractions, or extensive handling of nucleic acids.

Microarray Target Synthesis Kit (T7)

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