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Microarray Target Purification Kit and,,,the Microarray Target Synthesis Kit (T7)

As the human genome sequence project is completed, the vast amount of genomic information calls for methods that allow gene expression analysis on a genomic scale and speed up detection of significant sequence variations. The most suitable tools available for gene expression analysis are DNA hybridization arrays that allow researchers to examine differences in expression of hundreds or thousands of genes in parallel. That is why DNA arrays, on a variety of platforms such as macroarrays, microarrays or high-density oligonucleotide arrays make their way into different areas of functional genomics research. Arrays provide immobilized gene-specific sequences (probes) on different solid supports (e.g., nylon membranes, glass slides, or silicon/ceramic chips) and are hybridized with labeled targets that are copies of nucleic acids derived from various biological sources.



This requires reliable and efficient tools for preparation and labeling of targets: RNA needs to be extracted from small amounts of precious sample materials, and labeled either directly during cDNA preparation or via a linear amplification (100 to 1000 fold) with T7 polymerase producing labeled cRNA, or via polymerase chain reaction (PCR)-based amplification methods. This step must be highly reproducible and efficient to avoid differences in hybridization caused by biased amplification of target material.

Therefore, Roche Applied Science has developed a range of DNA microarray analysis products for expression profiling, suitable especially for very small amounts of starting material or when highest sensitivity is required. Each product is designed and tested in combination with the others to cover the critical
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ssDNA from PCR DNA in less than 1 hour. No gels, columns, or slurries. Sequence from high quality ssDNA: clearer reads, longer runs....
Applications: Immunohistochemistry, ISH, FISH, CISH....
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