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MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers

hat are down-regulated in the sample relative to the pool, and red shows miRNAs that are up-regulated in the sample relative to the pool. Clustering of the 26 samples based on the miRNA expression profiles is shown above the heat map.


The mirVana miRNA Detection Kit, which relies on a solution hybridization detection scheme optimized for detection of miRNA and other small RNAs, was used to measure miRNA expression levels of three of the miRNAs in total RNA samples from ten of the human tissues. As seen in Figure 5, the quantitative data from the miRNA array procedure is very similar to what was generated using the mirVana miRNA Detection Kit or Northern analysis. This indicated that the miRNA Array procedure is generating reliable quantitative data for miRNA analysis.

Figure 5. Independent Verification of Data from the miRNA Array Procedure. The expression of two different miRNAs was measured in ten different human total RNA samples using the mirVana miRNA Detection Kit or Northern analysis. 5S and 5.8S rRNAs were used as loading controls. The relative expression levels of the miRNAs in the specified tissue relative to the pooled sample from the miRNA Array experiment in Figure 4 are shown graphically below each gel. Panel A: let-7c. Panel B: miR-16.


miRNAs in Cancer Samples

The miRNA array procedure was also used to compare the miRNA expression profiles of tumor and normal adjacent tissues from patients with lung, colon, breast, bladder, and thyroid cancers. Cancer samples have miRNA expression profiles that are clearly distinct from normal tissues (Figure 6)
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