Navigation Links
MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers

hat are down-regulated in the sample relative to the pool, and red shows miRNAs that are up-regulated in the sample relative to the pool. Clustering of the 26 samples based on the miRNA expression profiles is shown above the heat map.


The mirVana miRNA Detection Kit, which relies on a solution hybridization detection scheme optimized for detection of miRNA and other small RNAs, was used to measure miRNA expression levels of three of the miRNAs in total RNA samples from ten of the human tissues. As seen in Figure 5, the quantitative data from the miRNA array procedure is very similar to what was generated using the mirVana miRNA Detection Kit or Northern analysis. This indicated that the miRNA Array procedure is generating reliable quantitative data for miRNA analysis.

Figure 5. Independent Verification of Data from the miRNA Array Procedure. The expression of two different miRNAs was measured in ten different human total RNA samples using the mirVana miRNA Detection Kit or Northern analysis. 5S and 5.8S rRNAs were used as loading controls. The relative expression levels of the miRNAs in the specified tissue relative to the pooled sample from the miRNA Array experiment in Figure 4 are shown graphically below each gel. Panel A: let-7c. Panel B: miR-16.


miRNAs in Cancer Samples

The miRNA array procedure was also used to compare the miRNA expression profiles of tumor and normal adjacent tissues from patients with lung, colon, breast, bladder, and thyroid cancers. Cancer samples have miRNA expression profiles that are clearly distinct from normal tissues (Figure 6)
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
2. Getting Started with MicroRNA Research
3. Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
4. The Flexible matriXarray Chip System: A New Perspective for Probe Optimization in Gene-Expression Profiling
5. Ambion Offers Gene Expression Profiling Service
6. Total RNA from Whole Blood for Expression Profiling
7. Unmask Blood RNA for Gene Expression Profiling
8. Proteomics in Bladder Cancer Research: Protein Profiling of Bladder Squamous Cell Carcinomas, Rev C
9. Microgenomic Expression Profiling
10. Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
11. A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays
Post Your Comments:
(Date:1/22/2015)... Denville, NJ (PRWEB) January 22, 2015 ... shearing technologies such as the Bioruptor® and complete ... semi-automation system for chromatin immunoprecipitation, alleviating the need ... reagents and controls needed for ChIP of histones ...
(Date:1/22/2015)... , Jan. 22, 2015  Derma Sciences, Inc. ... company focused on advanced wound care, announces that ... tissues, have been added to the Premier, Inc. ... the opportunity for the AMNIOEXCEL® and AMNIOMATRIX® product ...
(Date:12/24/2014)... Dec. 24, 2014   BioLife Solutions , Inc. (NASDAQ: ... proprietary clinical grade hypothermic storage and cryopreservation ... for cells and tissues  ("BioLife" or the "Company"), today announced ... on May 4, 2015 (the "Annual Meeting"). Because ...
(Date:12/24/2014)... Bethesda, MD (PRWEB) December 23, 2014 ... law H.R. 83, the Omnibus and Continuing Resolution Appropriations ... hydrocephalus a condition eligible to receive funding through the ... Department of Defense (DoD). The Hydrocephalus Association (HA), working ...
Breaking Biology Technology:Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 3Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 5BioLife Solutions Sets Date for Annual Meeting of Stockholders 2Rare Brain Condition Now Eligible for Department of Defense Research Dollars 2
... Bio Corporation says its scientists have developed a ... ,Antibodies, naturally part of the immune system, are manufactured ... or research. Mirus' new technique, described in the February ... whose immune systems produce the antibodies that are harvested ...
... — Inacom Information Systems was awarded ... company's annual partner summit in San Diego, California. , ... campaign, which the company said drew 47 interested companies ... an implementation of wireless technology. , ,The campaign began ...
... Cyber Defense Agency, an information security consulting ... said it has been chosen to challenge eight student ... are increasing in complexity and sophistication, so it's essential ... the next generation of cyber defenders," said Sami Saydjari, ...
Cached Biology Technology:
(Date:12/19/2014)...   LaunchKey , the first decentralized mobile authentication and ... Things era, today announced the close of $3 million ... by Metamorphic Ventures with participation from ENIAC Ventures, Crosslink ... LaunchKey has raised $4 million to date, and will ...
(Date:12/17/2014)... Research and Markets ( http://www.researchandmarkets.com/research/9zq33j/global_chemical ) has ... Market 2015-2019" report to their offering. ... trend upcoming in this market is the increasing demand ... in recording of patient data for quick and correct ...
(Date:12/11/2014)... N.C. , Dec. 10, 2014  That blood pressure plays ... a while. Hypertension – the medical term for high blood ... early 1800s, and the inflatable cuff that,s used in measuring ... however, mean there,s nothing new about hypertension, its triggers and ...
Breaking Biology News(10 mins):LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 3Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4
... of deaths in the United States every year. The disease ... supply blood to the heart muscle. This occurs as a ... to progressive buildup of fatty plaque near the surface of ... month in the SIAM Journal on Applied Mathematics , ...
... Dec. 9, 2013  Bruin Biometrics, LLC ("BBI") an ... has received ISO 13485:2003 and EN ISO 13485:2012 ... System with its notified body SGS. Receipt of ... in the design, development, manufacture, and distribution of ...
... As embryonic tissue develops, cells push and pull ... for the tissue to develop properly. Now scientists at ... these forces in three-dimensional (3D) tissues and living embryos. ... lead to new tools to diagnose cancer, hypertension, connective ...
Cached Biology News:Math models enhance current therapies for coronary heart disease 2Math models enhance current therapies for coronary heart disease 3Measuring life's tugs and nudges 2Measuring life's tugs and nudges 3
Mouse anti-IkBa/MAD-3 Class: Antibody Product Group: Signalling molecules and phospho-specific Antibody...
Rabbit polyclonal to Oct2, prediluted ( Abpromise for all tested applications). entrezGeneID: 5452 SwissProtID: P09086...
Anti-Mouse Reticular Fibroblast, Purified (Clone ER-TR7) (rat IgG2a)...
Mouse monoclonal [2Q2147] to Aequorin ( Abpromise for all tested applications)....
Biology Products: