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MessageAmp II: Use Less RNA for Array Analysis

f RNA input levels are below ~5075 ng.

Figure 4. aRNA Yields After Two Rounds of Amplification. Plot of typical aRNA yields (triplicates determined by UV absorbance) for 1000, 100, 50, and 10 pg of input HeLa cell total RNA after two rounds of amplification. In vitro transcription reactions were performed for 16 hr for both rounds.


An Improved Kit for aRNA Amplification
The inclusion of ArrayScript in conjunction with the optimization of the second-strand cDNA synthesis reaction in the MessageAmp II Kit results in increased conversion of mRNA into full-length, double-stranded cDNA templates. The kit also uses Ambions MEGAscript IVT technology, generating enough cRNA for GeneChip analysis from as little as 100 ng of input RNA in a single round of amplification.

The MessageAmp II aRNA Amplification Kit contains all necessary reagents and components for first-strand cDNA synthesis, RNase H digestion, second-strand synthesis, cDNA purification, in vitro transcription, and aRNA purification. Reagents for 20 reactions and a detailed Instruction Manual are included.


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