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MessageAmp II: Use Less RNA for Array Analysis


Amplifies as little as 100 ng RNA in a single round
High Percent Present Calls and low 3'/5' ratios in GeneChip analysis
Includes ArrayScript M-MLV RT engineered to maximize yields of full-length cDNA
The best in vitro transcription technology available


Better Reverse Transcription and cDNA Synthesis
Ambions MessageAmp aRNA Amplification Kit was the first commercially available Eberwine-based RNA amplification kit. The next generation MessageAmp II aRNA Amplification Kit (patent pending) incorporates extensive improvements to the original kit.

In developing MessageAmp II, the reverse transcription and second strand cDNA synthesis steps, both critical for generating high yields of full-length labeled aRNA, were optimized. Major improvements include the development of ArrayScript M-MLV RT (see ArrayScript RT: Maximize Yields of Full-Length cDNA). ArrayScript is an engineered M-MLV reverse transcriptase that synthesizes exceptionally high yields of full-length cDNA unattainable with wild type enzymes. The second strand cDNA synthesis reaction was also further optimized to convert the cDNA to double-stranded cDNA with high efficiency. As a result, as little as 100 ng of total RNA in a single ro
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