Navigation Links
Measuring siRNA-mediated knockdown of IL-8 mRNA

100 l fresh complete DMEM. The QuantiGene Lysis Working Reagent was added to each treatment group for an additional 30 minutes. The QuantiGene assay was performed according to manufacturers instructions.


RESULTS
HeLa cells induced with PMA elicit a robust increase in the IL-8 expression. Based on the experimental results (data not shown), we determined that Cyclophilin B (PPIB) demonstrated more stable expression than GAPDH and subsequently was used as the normalization control. Following stimulation and siRNA transfection, the mRNA levels of each gene were measured from four replicate treatments of each experimental condition. The results are shown in Figure 2.

Coefficients of variation (CVs) measured from different treatments ranged from 5 to 15% over a 3-log range of relative luminescence (RLU) values. These CVs allow for the clear distinction of the effects of the siRNA seen at four hours (89% knockdown) from the effects seen at eight hours (94% knockdown). To normalize the results, the IL-8 and Cyclophilin B measurements made from the same replicate (i.e., the same batch of lysed cells) were converted to a ratio (IL-8 RLU to Cyclophilin B RLU). This ratio was calculated for each of the four replicates for each of the four experimental conditions. The results of this analysis are shown in Figure 3, where the error bars indicate one standard deviation. Following normalization, the range of CVs was determined to be 3 to 15%.


CONCLUSIONS
In the study described above, the QuantiGene Reagent System demonstrated a high level of precision and reproducibility in experiments that are commonly performed by researchers who utilize RNAi. CVs ranged from 5 to 15%, and from 3 to 15% when normalized to the expression of an endogenous control gene. The results of these experiments demonstrate that the QuantiGene Reagent System is a powerful companion t
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Measuring multiple apoptosis parameters with the Agilent 2100 bioanalyzer
2. Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation
3. Measuring RNase Activity A Real-time Kinetic Analysis
4. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Measuring siRNA mediated knockdown mRNA

(Date:3/17/2010)... ... design their own unique shelter solution. , ... Orangeburg, NY (Vocus) March 17, 2010 -- DHS Technologies recently launched a newly ... , , , ,VTAP 2.0, or the second edition of the Virtual Tactical Application Program, ...
(Date:3/16/2010)... Americans living with colorectal cancer will develop liver metastases ... Radiofrequency ablation, a minimally invasive treatment that applies heat ... minimal associated injury to the surrounding healthy liver, contributes ... note researchers at the Society of Interventional Radiology,s 35th ...
(Date:3/16/2010)... ... ... EDMONTON , March 16 ... ...
(Date:3/16/2010)... (DDSU; www.ddstechnologiesusa.com ) had retained the Mina Mar Group ( http://www.minamargroup.com/ ) as forefront representative of the company,s new expansive corporate interests. , ... , ... ... ...
Breaking Biology Technology:DHS Technologies Launches VTAP 2.0 2Living longer: Colon cancer patients gain time with radiofrequency ablation treatment 2Living longer: Colon cancer patients gain time with radiofrequency ablation treatment 3BioMS Medical Announces 2009 Year End Results 2BioMS Medical Announces 2009 Year End Results 3BioMS Medical Announces 2009 Year End Results 4BioMS Medical Announces 2009 Year End Results 5BioMS Medical Announces 2009 Year End Results 6BioMS Medical Announces 2009 Year End Results 7BioMS Medical Announces 2009 Year End Results 8BioMS Medical Announces 2009 Year End Results 9BioMS Medical Announces 2009 Year End Results 10DDS Technologies USA, Inc. (DDSU) Appoints Mina Mar Group as Its Corporate Advisor for a Merger 2DDS Technologies USA, Inc. (DDSU) Appoints Mina Mar Group as Its Corporate Advisor for a Merger 3DDS Technologies USA, Inc. (DDSU) Appoints Mina Mar Group as Its Corporate Advisor for a Merger 4DDS Technologies USA, Inc. (DDSU) Appoints Mina Mar Group as Its Corporate Advisor for a Merger 5DDS Technologies USA, Inc. (DDSU) Appoints Mina Mar Group as Its Corporate Advisor for a Merger 6
... proprietary adult stem cell bone graft product and processing ... facility -, - Expects Osteocel(R) ... to earnings per share in 2008 or 2009, excluding ... in-process R&D charge -, SAN DIEGO, May 8 NuVasive, Inc. (Nasdaq:,NUVA), ...
... 8 Pharmos Corporation,(Nasdaq: PARS ) today reported results ... a net loss of $3.6 million, or $0.14 per share,for ... $4.8 million, or $0.19,per share, in the first quarter 2007. ... The decrease in net loss for the first quarter ...
... Edwards Lifesciences,Corporation (NYSE: EW ), the world leader ... will celebrate a number of cardiac surgery,milestones and product ... for Thoracic Surgery (AATS), May 10-14 in San Diego., ... this year celebrates,its 50th anniversary of partnering with clinicians ...
Other Biology Technology:NuVasive Signs Definitive Agreement to Acquire Osteocel Biologics Business from Osiris Therapeutics 2NuVasive Signs Definitive Agreement to Acquire Osteocel Biologics Business from Osiris Therapeutics 3NuVasive Signs Definitive Agreement to Acquire Osteocel Biologics Business from Osiris Therapeutics 4NuVasive Signs Definitive Agreement to Acquire Osteocel Biologics Business from Osiris Therapeutics 5NuVasive Signs Definitive Agreement to Acquire Osteocel Biologics Business from Osiris Therapeutics 6Pharmos Corporation Reports 2008 First Quarter Results 2Pharmos Corporation Reports 2008 First Quarter Results 3Pharmos Corporation Reports 2008 First Quarter Results 4Edwards Lifesciences Celebrates One Million Heart Valve Patients and More at AATS 2008 2Edwards Lifesciences Celebrates One Million Heart Valve Patients and More at AATS 2008 3Edwards Lifesciences Celebrates One Million Heart Valve Patients and More at AATS 2008 4
(Date:3/21/2010)... new energy source so controversial that people once regarded ... by the mainstream scientific community. That,s the conclusion of ... on the topic "cold fusion" being held ... Center during the 239th National Meeting of the American ... afraid to speak about ,cold fusion, to a mainstream ...
(Date:3/18/2010)... is available in Spanish . , Feeding the ... their regular diet makes them less aggressive and easier to ... scientists and cooperators. , The tryptophan-enhanced diet reduced aggression and ... study. Tryptophan, which is only acquired through diet, is the ...
(Date:3/18/2010)... , , Michael M. Graham, Ph.D., M.D., president, ... chair, SNM Medical Isotope Task Force, Jeffrey P. Norenberg, ... member, SNM Medical Isotope Task Force, , WHEN:,Immediately ... over a two-week period when cancer, heart and brain ...
Breaking Biology News(10 mins):'Cold fusion' moves closer to mainstream acceptance 2'Cold fusion' moves closer to mainstream acceptance 3'Cold fusion' moves closer to mainstream acceptance 4Tryptophan-enriched diet reduces pig aggression 2Stephen Coles is Recognized by Continental Whos Who Among Health Care Research Professionals 15730 1Stephen Coles is Recognized by Continental Whos Who Among Health Care Research Professionals 15730 2HIMSS Honors Memorial Healthcare System for Using TheraDoc 28TM 29 Decision Support Technology to Achieve 97 Percent Compliance With Pediatric Asthma 8076 1HIMSS Honors Memorial Healthcare System for Using TheraDoc 28TM 29 Decision Support Technology to Achieve 97 Percent Compliance With Pediatric Asthma 8076 2HIMSS Honors Memorial Healthcare System for Using TheraDoc 28TM 29 Decision Support Technology to Achieve 97 Percent Compliance With Pediatric Asthma 8076 3HIMSS Honors Memorial Healthcare System for Using TheraDoc 28TM 29 Decision Support Technology to Achieve 97 Percent Compliance With Pediatric Asthma 8076 4HIMSS Honors Memorial Healthcare System for Using TheraDoc 28TM 29 Decision Support Technology to Achieve 97 Percent Compliance With Pediatric Asthma 8076 5HIMSS Honors Memorial Healthcare System for Using TheraDoc 28TM 29 Decision Support Technology to Achieve 97 Percent Compliance With Pediatric Asthma 8076 6HIMSS Honors Memorial Healthcare System for Using TheraDoc 28TM 29 Decision Support Technology to Achieve 97 Percent Compliance With Pediatric Asthma 8076 7If bonobo Kanzi can point as humans do what other similarities can rearing reveal 3F 12099 1If bonobo Kanzi can point as humans do what other similarities can rearing reveal 3F 12099 2If bonobo Kanzi can point as humans do what other similarities can rearing reveal 3F 12099 3
... recycling system has been developed that allows water to ... communal waste water system. , The Green Roof Water ... washing water, which can then be reused for activities ... the brainchild of Chris Shirley-Smith, whose company Water Works ...
... the immune-stimulating hormone known as interleukin-12 (IL-12) can ... as an experimental therapy for some cancers. ... the immune system to attack certain cancers. This ... to treat melanoma, advanced kidney cancer and other ...
... genetic interactions promises to change how researchers study the ... is called epistatic mini array profiles (E-MAP), has already ... to uncover the roles of previously uncharacterized proteins, and ... one another. , E-MAP will enable new understanding ...
Other Biology News:GROWing the next generation of water recycling plants 2Study shows combination of immune substances to be safe 2Technique offers new view of dynamic biological landscape 2Technique offers new view of dynamic biological landscape 3Technique offers new view of dynamic biological landscape 4
... Freedom EVO96R and Freedom EVO384R are fast ... available in three different sizes, with a ... They deliver significant time saving, with true ... easy setup of your assays with the ...
... Freedom EVO96R and Freedom EVO384R are fast ... available in three different sizes, with a ... They deliver significant time saving, with true ... easy setup of your assays with the ...
Prepared by multi-temperature ethanol fractionation with extensive charcoal treatment and dialysis.• Assay: ≥ 95 % (HPCE)• pH ~5.2 (10 mg/mL in 0.15 M NaCl)...
... number is a new product number, ... number. If showing no availability yet, ... number (Z71,405-4) or contact customer service ... mm volume diam. 3 mL ...
Biology Products: