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Measuring multiple apoptosis,,,parameters with the Agilent 2100,,,bioanalyzer

samples in figure 4 turned out to be 35 % apoptotic as confirmed by the annexin V assay. A clear discrimination of samples that contain a lower number of apoptotic cells is achieved by overlaying negative controls and samples in the electropherogram view (figure 5).


If alternative DNA purification methods are used, special attention must be paid as the injection of large amounts of genomic DNA (like whole cell lysates) may lead to clogging of the chip. The recommended extraction protocol eliminates such interference of genomic DNA. In addition, fragmented DNA is concentrated, which permits the apoptosis measurement of low cell numbers.


Conclusion
A combination of complementary assays is usually required to confirm that cells are apoptotic. The 2100 bioanalyzer offers the flexibility to quantitatively measure flow cytometric parameters and subsequently perform a DNA fragmentation assay. It eliminates the need for outsourcing samples and helps keep consolidated electronic track of all results. In addition to faster results and ease-of-use, the high sensitivity and optimized protocols allow minimum cell consumption and saves sample preparation time.



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