Measuring multiple apoptosis,,,parameters with the Agilent 2100,,,bioanalyzer
(Sigma #C9911) in medium. Cells were harvestedand counted at the indicatedtime intervals. Cell lysis andDNA purification was performedas described in the fast protocolbelow. Eluted DNA was directlyloaded into the chip wells preparedaccording to the LabChipReagent Kit Guide. Fast protocol for DNA fragmentpurification (based on original
C9911) in medium. Cells were harvested
and counted at the indicated
time intervals. Cell lysis and
DNA purification was performed
as described in the fast protocol
below. Eluted DNA was directly
loaded into the chip wells, prepared
according to the LabChip
Reagent Kit Guide.
Fast protocol for DNA fragment
purification (based on original
protocol in reference 11)
Pellet 1-2 106 cells by centrifugation
(2 min 400 g).
Add 100 L 4 C lysis solution
(0. 2% Triton X, 10 mM Tris, 10
mM EDTA) and gently resuspend.
Incubate 5 min at 4 C.
Centrifuge for 5 min at 13000 g.
Transfer supernatant to new
vial and discard the pellet.
Purify DNA using a low volume
elution method. Here a PCR
purification kit* was used
(QIAGEN MinElute PCR Purification
Run in Agilent 2100 bioanalyzer
with the DNA 12000 LabChip
Kit, selecting the DNA 12000
Laddering assay from the biosizing
*As an alternative to the PCR
purification kit, the following protocol
has been successfully tested:
Add 2 L 5M NaCl to the lysate
and vortex 5 seconds.
Add 200 L ice-cold absolute
ethanol and vortex 5 seconds.
Incubate on ice for 10 minutes
Centrifuge at 13000 g for 5 minutes.
Dry residual ethanol in speedvac
Resuspend precipitate in 30 L
of pre-warmed (65 C) distilled
water or 10 mM Tris/10 mM
Run in Agilent 2100 bioanalyzer
with the DNA 12000 LabChip
kit, selecting the DNA 12000
laddering assay. UV measurements
UV spectroscopy readings were
performed to determine total DNA
concentration. The Agilent 8453
UV-visible spectrophotometer '"/>
(Date:1/15/2014)... Toronto, Canada (PRWEB) January 15, 2014 ... FDA regulatory perspectives on nonclinical and clinical safety ... many countries to guide a path for biosimilar ... makes the demonstration of biosimilarity with regards to ... the specific aspects of biosimilar drug development and ...
(Date:1/15/2014)... The Microcompetition with Foreign DNA theory explains how ... these latent viruses is the Epstein Barr Virus (EBV), ... Rheumatoid arthritis (RA) is a chronic inflammatory disease that ... a study found that RA patients have high concentrations ... What is Microcompetition? , Dr. Hanan Polansky discovered that ...
(Date:1/15/2014)... AudioNotch is the internet's leading provider of ... treatment of tinnitus. Patients listen to sound therapy ... over a period of weeks to months, their tinnitus ... Therapy in two forms: Notched Music and Notched White ... of a new form of Tailor-Made Notched Sound Therapy: ...
(Date:1/14/2014)... San Diego, CA (PRWEB) January 14, 2014 ... developing solutions based on the products of cells ... they have entered into an international license agreement ... containing Histogen’s proprietary multipotent cell conditioned media (CCM). ... to the existing license between Histogen and Suneva ...
... 5, 2011 ZyGEM Corp. Ltd., a developer and ... and other nucleic acids, today announced publication of an ... kit can rapidly extract DNA from small samples of ... produce accurate, reliable and reproducible DNA profiles for forensic ...
... Ariz., Oct. 4, 2011 The VENTANA anti-Helicobacter pylori ... first H. pylori antibody to ... (FDA). Developed by Ventana Medical Systems, Inc. (Ventana), a ... antibody, when used in immunohistochemical (IHC) staining, aids in ...
... 2011 Northwest Biotherapeutics (OTC.BB: NWBO) (NORTHWEST BIO) ... the second annual Cancer Immunotherapy: A Long-Awaited Reality ... York Academy of Medicine in New York City. ... executives, and other stakeholders to engage in discussions, ...
(Date:3/13/2014)... How does the ... record 3D X-ray films showing the internal movement dynamics in ... the temporal dimension has now been developed by researchers at ... to a living weevil. From up to 100,000 two-dimensional radiographs ... time or slow motion. The results are now published in ...
(Date:3/13/2014)... that tropical grassy areas, which play a critical role ... result of ineffective management. , According to research, published ... are often misclassified and this leads to degradation of ... plants and animals that are indigenous to these areas. ... area than tropical rain forests, support about one fifth ...
(Date:3/13/2014)... let the cat out and started the dishwasher. Or ... not just what happened and where, but when an ... New research from the University of California, Davis, Center ... called the hippocampus stores memories by their "temporal context" ... "We need to remember not just what happened, but ...
... From the crops we farm to the insects which ... with nature, commanding some species while falling victim to ... and Nick Battey explore the subject of biodiversity through ... exploit humans. The authors selected 18 such organisms ...
... for World Health and Medicine at Saint Louis University and ... agreement to develop new drugs to combat diarrhea, which is ... age 5. Each year more than two million children ... by a wide range of bacterial, parasitic and viral pathogens. ...
... from the Smithsonian Institution, discovered that leaves of flowering ... per unit area than leaves ever had before. They ... to the leaves, making it possible for plants to ... system may also have radically altered water and carbon ...
Multi-enzyme complex containing a wide range of carbohydrases, including arabanase, cellulase,b-glucanase, hemi-cellulase and xylanase Patent/trademark/license (electronic): A product of Novozyme Cor...
Miscellaneous: Versatile stain for staining RNA (bluish-purple), DNA (blue), proteins (red) and acid polysaccharides Assay: ~ 95 % Suitab tst: differential staining of nucleic acids and prote...
... EPICENTREs patented MasterAmp 10X PCR Enhancer ... and allows improved sensitivity and specificity ... Using MasterAmp PCR Enhancer in PCR ... DNA melting, increases enzyme?s thermal stability, ...