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Measuring multiple apoptosis,,,parameters with the Agilent 2100,,,bioanalyzer

(Sigma, # C9911) in medium. Cells were harvested and counted at the indicated time intervals. Cell lysis and DNA purification was performed as described in the fast protocol below. Eluted DNA was directly loaded into the chip wells, prepared according to the LabChip Reagent Kit Guide.
Fast protocol for DNA fragment purification (based on original protocol in reference 11)
Pellet 1-2 106 cells by centrifugation (2 min 400 g). Remove medium.
Add 100 L 4 C lysis solution (0. 2% Triton X, 10 mM Tris, 10 mM EDTA) and gently resuspend.
Incubate 5 min at 4 C. Centrifuge for 5 min at 13000 g.
Transfer supernatant to new vial and discard the pellet.
Purify DNA using a low volume elution method. Here a PCR purification kit* was used (QIAGEN MinElute PCR Purification Kit. Cat.28004).
Run in Agilent 2100 bioanalyzer with the DNA 12000 LabChip Kit, selecting the DNA 12000 Laddering assay from the biosizing software.

*As an alternative to the PCR purification kit, the following protocol has been successfully tested:
Add 2 L 5M NaCl to the lysate and vortex 5 seconds.
Add 200 L ice-cold absolute ethanol and vortex 5 seconds.
Incubate on ice for 10 minutes Centrifuge at 13000 g for 5 minutes. Discard supernatant.
Dry residual ethanol in speedvac or desiccator.
Resuspend precipitate in 30 L of pre-warmed (65 C) distilled water or 10 mM Tris/10 mM EDTA.
Run in Agilent 2100 bioanalyzer with the DNA 12000 LabChip kit, selecting the DNA 12000 laddering assay.
UV measurements
UV spectroscopy readings were performed to determine total DNA concentration. The Agilent 8453 UV-visible spectrophotometer
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