Navigation Links
Measuring multiple apoptosis,,,parameters with the Agilent 2100,,,bioanalyzer

used by nature during development6, homeostasis, aging and in defense mechanisms. Apoptosis is a key factor in cancer and also suspected to be a characteristic mechanism for degenerative disorders such as Alzheimer7 or Parkinson's diseases. The process is universal and has been described in eukaryotic cells as well as in individual bacteria8, protozoa9 and amoeba10. It is a target for research scientists in a wide variety of fields. Apoptosis is characterized by a distinct set of morphological events involving plasma membrane blebbing and asymmetry loss, reduction of cell volume, loss of mitochondrial membrane potential, nuclear condensation, fragmentation of DNA at nucleosomal intervals and other cytoplasmatic changes. Ultimately fragmentation of the cell into membrane-enclosed apoptotic bodies occurs. Differential expression patterns or alternate mechanisms in the apoptosis pathways among tissues and cell lines makes it necessary to have two or even three parallel experiments to confirm, quantitate and compare kinetic events on apoptosis. The appearance of DNA laddering is unambiguously connected to apoptosis. Targeting of phosphatidyl serine (PS) in the outer leaflet of cell membrane with fluorescently labeled annexin V and antibody labeling of active-caspase- 3 in cells has been previously described in conjunction with the Agilent 2100 bioanalyzer as a quantitative measurement for apoptotic cell samples5. This Application Note describes the combined measurement of annexin V and the extracted DNA of apoptotic cells on one platform the Agilent 2100 bioanalyzer. A fast protocol for DNA preparation and recommendations for data evaluation are discussed.
Experimental
Apoptosis was induced in Jurkat cells by incubation in a 5 M solution of the topoisomerase inhibitor Camptothecin
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation
2. Measuring RNase Activity A Real-time Kinetic Analysis
3. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
4. Measuring siRNA-mediated knockdown of IL-8 mRNA
5. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
6. Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori
7. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
8. Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer
9. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer
Post Your Comments:
(Date:7/30/2015)... MN (PRWEB) , ... July 31, 2015 , ... Ralco ... Enrichment Center at the Lyon County Fair to be held August 5-9 in Marshall. ... about where their meals come from and how agriculture impacts their daily lives. This ...
(Date:7/30/2015)... , ... July 30, 2015 , ... The 2015 Market ... study on the current state of the global Propanol market with a focus on ... BASF, Eastman, Dow, Carboclor, Mitsui Chemicals, Oxea, ISU Chemical, Sanofi, Royal Dutch Shell, LG ...
(Date:7/30/2015)... , Inc. (NASDAQ: SNMX ), a leading company ... commercialize novel flavor ingredients for the food, beverage, and ... second quarter 2015. "Moving into the ... achieve our commercial and financial goals," stated John Poyhonen, ... our last quarterly earnings report, our two partners have ...
(Date:7/30/2015)... July 30, 2015  Discovery Laboratories, Inc. (Nasdaq: ... received the second $1.0M tranche under a previously ... grant valued at up to $3.0 million to ... surfactant as a potential medical countermeasure to mitigate ... was awarded an initial $1.0 million under this ...
Breaking Biology Technology:Ralco Announces Primary Sponsorship of Ralco Enrichment Center at Lyon County Fair 2Global Propanol Market Technical Data and Manufacturing Analysis 2020 2Global Propanol Market Technical Data and Manufacturing Analysis 2020 3SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 2SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 3SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 4SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 5SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 6SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 7SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 8SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 9Discovery Labs Receives $1.0 million under an SBIR Grant in Continuing Support of the Development of Aerosolized KL4 Surfactant to Address Radiation-Induced Lung Injury 2Discovery Labs Receives $1.0 million under an SBIR Grant in Continuing Support of the Development of Aerosolized KL4 Surfactant to Address Radiation-Induced Lung Injury 3
... Enerkem,s future biofuels plant will supply green energy to ... - Enerkem, a leading waste-to-biofuels and green chemicals technology company, ... that will heat a Strathcona County neighbourhood, using the residual ... Edmonton waste-to-biofuels plant. , The project received grant funding in ...
... ... suite of four STR kit configurations designed to fulfill latest DNA database standards ... Standard Set and other routinely tested loci, plus the five loci recently recommended ... alpha test labs, and enables easier database sharing and standardization. , ...
... ... Artemis Health Institute. , ... India (PRWEB) October 1, 2009 -- Artemis Health Institute has entered into a partnership with ... The partnership will focus on three core areas -- clinical care, research and training. ...
Cached Biology Technology:Enerkem Announces Unique Community Energy Project 2New STR Systems from Promega Comply with Latest DNA Database Standards 2Memorial Sloan-Kettering Selects Artemis as Affiliate for India 2Memorial Sloan-Kettering Selects Artemis as Affiliate for India 3
(Date:7/2/2015)... 25, 2015 Research ... of the "Natural Language Processing Market by ... OCR, Pattern & Image Recognition) - Worldwide Forecast ... The key vendors occupying the market are ... Microsoft Corporation, NetBase Solutions, SAS Institute Inc., Verint ...
(Date:6/30/2015)... To bolster its efforts and commitment to fighting cyber fraud, ... of two new team members. Dimitri ... Raviv will act as head of strategic alliances. These ... the most secure solutions for the enterprise market. ... a provider of security and management products, which was acquired ...
(Date:6/26/2015)... , June 26, 2015 ATL Technology, LLC, ... devices with specialties in single use solutions, headquartered in ... MedConx, Inc. (a California corporation with ... San Jose, Costa Rica ). ... Costa Rica with ATL Technology,s existing facility in ...
Breaking Biology News(10 mins):Natural Language Processing Market by Type (Rule-Based, Statistical, and Hybrid), Technologies (Recognition, IVR, OCR, Pattern & Image Recognition) - Worldwide Forecast to 2020 2HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 2HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 3ATL Technology Announces Acquisition of MedConx, Inc. and Expanded Global Footprint 2
... Alternative energy is all the rage in major media headlines, ... to a study by researchers from Denmark and The Netherlands ... FASEB Journal, the brain, just like muscles, works harder ... glucose. Not only does this finding help explain why the ...
... Letters, Steve Brusatte, Professor Michael Benton, and colleagues ... not proliferate immediately after they originated, but that their ... by two mass extinctions. "The sheer size ... something special about these animals that preordained them for ...
... future of the gorgeously-decorated fish which throng Australia,s coral reefs ... tourism may well be written in their genes. Of ... are able to re-colonise regions of reef devastated by global ... corals move south, says Dr Line Bay of the ARC ...
Cached Biology News:Mass extinctions and the evolution of dinosaurs 2Fishy future written in the genes 2Fishy future written in the genes 3
Rabbit Anti-melatonin (different linkage) Polyclonal Antibody...
... Goat polyclonal to Cholesterol Oxidase ... / Specificity Cross-reacts with bacteria. Not ... Information Cholesterol oxidases exist as both ... are implicated in bacterial pathogenesis. In addition, ...
Anti-ACTH Immunogen: Porcine ACTH conjugated to BSA. Available Date: 38566...
Goat polyclonal to XAGE1 ( Abpromise for all tested applications). Antigen: Synthetic peptide: CGFGFRRQGEDNT, corresponding to C terminal amino acids 149-160 of Human XAGE1 Entrez Gene ID: 9...
Biology Products: