Navigation Links
Measuring multiple apoptosis,,,parameters with the Agilent 2100,,,bioanalyzer

A simplified and fast protocol for the analysis of DNA fragmentation during apoptosis
This Application Note describes a simplified and fast protocol for apoptosis DNA fragmentation analysis with the Agilent 2100 bioanalyzer using the DNA LabChip kit. The combination of Annexin V, activecaspase- 3 and DNA laddering analysis on the same platform together with the Cell Fluorescence LabChip kit permits quick confirmation and quantitation of apoptosis in cell populations. A fast and simple protocol was applied for the extraction of fragmented, chromosomal DNA. The increased sensitivity and sizing accuracy of the DNA LabChip kit compared to conventional agarose gel electrophoresis also means that a significant lower amount of apoptotic cells is required for the analysis.
The Agilent 2100 bioanalyzer was introduced by Agilent Technologies as the first commerially available lab-on-a-chip analysis system for the life science laboratory using LabChip products, developed in collaboration with Caliper Technologies Corp. Chip-based approaches for a variety of separation- based techniques have been introduced, addressing DNA, RNA and protein separations.1, 2, 3. Recently the Agilent 2100 bioanalyzer has been extended to enable dual color simple flow cytometric assays4. Apoptosis assays have been demonstrated for quantitative measurements of whole cells5. The combination of cytometric and gel-like separations on the same instrument platform makes it an ideal tool for multi-parametric apoptosis quantitation and confirmation.
Apoptosis
Apoptosis, or programmed cell death, is the outcome of a metabolic cascade that results in cells dying in a controlled manner. It is
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation
2. Measuring RNase Activity A Real-time Kinetic Analysis
3. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
4. Measuring siRNA-mediated knockdown of IL-8 mRNA
5. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
6. Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori
7. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
8. Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer
9. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer
Post Your Comments:
(Date:9/1/2014)... NewYork (PRWEB) September 01, 2014 ... Biotech and Diagnostics report provides comprehensive understanding and ... agreements entered into by the worlds leading life ... understand and analysis of how and why companies ... of a prospective partner’s negotiated deals terms provides ...
(Date:8/31/2014)... 31, 2014 Wockhardt Limited today announces a ... Anti-Infective research when two of its drugs, WCK 771 ... Product (QIDP) status from U.S. Food & Drug Administration ... against pathogens which have a high degree of unmet ... for Disease Control (a top U.S. government health and ...
(Date:8/31/2014)... Available spectra is similar for all ... cultivation of a wide range of plants. The ... proven patented and pending wide spectrum technology. The ... critical applications in contrast to growth spectra made ... have a large variety of differently sized and ...
(Date:8/31/2014)... 2014 Market Research Report ... and in-depth research report on the Polyacrylamide ... including Polyacrylamide definition, classification, application, and industry ... This research covers the international market analysis, ... industry analysis covering macroeconomic environment & economic ...
Breaking Biology Technology:Collaborative R&D Terms & Agreements in Pharma, Biotech and Diagnostics Industry Analysis, Size, Share, Growth, Trends And Forecast Research Report by Researchmoz.us 2Collaborative R&D Terms & Agreements in Pharma, Biotech and Diagnostics Industry Analysis, Size, Share, Growth, Trends And Forecast Research Report by Researchmoz.us 3Collaborative R&D Terms & Agreements in Pharma, Biotech and Diagnostics Industry Analysis, Size, Share, Growth, Trends And Forecast Research Report by Researchmoz.us 4U.S. FDA Grants QIDP Status for Wockhardt Anti-Infective Drug Discovery Program 2U.S. FDA Grants QIDP Status for Wockhardt Anti-Infective Drug Discovery Program 3International Polyacrylamide Industry & Copper Iodide Market Data Analysis Now Available at DeepResearchReports.com 2International Polyacrylamide Industry & Copper Iodide Market Data Analysis Now Available at DeepResearchReports.com 3
... , , , , BOULDER, Colo. and THOUSAND OAKS, ... and Amgen Inc.(Nasdaq: AMGN ) today announced that they entered ... glucokinase activator program, including ARRY-403, currently being tested in a Phase ... , Under the terms of the agreement, Array will receive an ...
... , JUPITER, Fla., Dec. 14 Dyadic International, Inc. (Pink ... collaboration with The Scripps Research Institute ("Scripps") by entering into ... patented and proprietary C1 fungus ("C1"). , This agreement is ... which began in March 2006 when Dyadic engaged Scripps to ...
... , , , ROCKAWAY, N.J., ... Nursing: An Evidence-Based Approach , published by ... treatment for anthracycline extravasation.(1) Anthracyclines are a group of ... been used in the treatment of various types of cancer ...
Cached Biology Technology:Array BioPharma and Amgen Partner in Type 2 Diabetes 2Array BioPharma and Amgen Partner in Type 2 Diabetes 3Array BioPharma and Amgen Partner in Type 2 Diabetes 4Array BioPharma and Amgen Partner in Type 2 Diabetes 5Array BioPharma and Amgen Partner in Type 2 Diabetes 6Array BioPharma and Amgen Partner in Type 2 Diabetes 7Dyadic International Extends Collaboration With The Scripps Research Institute 2Dyadic International Extends Collaboration With The Scripps Research Institute 3Dyadic International Extends Collaboration With The Scripps Research Institute 4Dyadic International Extends Collaboration With The Scripps Research Institute 5Infusion Nurses Society References Totect(R) (Dexrazoxane For Injection) as the Only Antidote for the Treatment of Anthracycline Extravasation 2Infusion Nurses Society References Totect(R) (Dexrazoxane For Injection) as the Only Antidote for the Treatment of Anthracycline Extravasation 3Infusion Nurses Society References Totect(R) (Dexrazoxane For Injection) as the Only Antidote for the Treatment of Anthracycline Extravasation 4Infusion Nurses Society References Totect(R) (Dexrazoxane For Injection) as the Only Antidote for the Treatment of Anthracycline Extravasation 5
(Date:9/1/2014)... EDT) It may be possible to train the ... foods, according to new research by scientists at the ... (USDA HNRCA) at Tufts University and at Massachusetts General ... & Diabetes , a brain scan study in adult ... reverse the addictive power of unhealthy food while also ...
(Date:9/1/2014)... Researchers reveal how the alteration of a single nucleotidethe basic building ... form of intellectual disability. The study appears in The ... syndrome is caused by a defect in a gene on the ... Around 1 in 230 women and 1 in 360 men carry ... one end of the FMR1 gene is slightly longer ...
(Date:9/1/2014)... revealed the presence of tiny blob-like structures that form ... don,t know what they do even though these ... crucial to the life of a cell, and therefore ... the Journal of Cell Biology , two researchers ... from biophysics to cell biology, to focus their attention ...
Breaking Biology News(10 mins):Training your brain to prefer healthy foods 2A nucleotide change could initiate fragile X syndrome 2Scientists call for investigation of mysterious cloud-like collections in cells 2
... women are encouraged to quit smoking when they become ... transdermal patches, nasal spray or lozenges. ... has shown that nicotine from either smoking or NRT ... the offspring, including an increased risk of obesity and ...
... Neb., Jan. 16, 2014 -- Using a camera-equipped robot ... scientists and engineers with the Antarctic Geological Drilling (ANDRILL) ... thousands of small sea anemones were burrowed into the ... frigid water like flowers on a ceiling. , ...
... American Society for Microbiology Conference for Undergraduate Educators (ASMCUE) ... DoubleTree by Hilton Boston North Shore in Danvers, Mass. ... leading microbiology and biology faculty and researchers while learning ... This year ASMCUE expands its collaborative program ...
Cached Biology News:Fetal exposure to nicotine increases long-term risk of obesity 2Ice-loving sea anemones discovered in Antarctica 2Ice-loving sea anemones discovered in Antarctica 32014 ASMCUE and ASM General Meeting collaborate to offer the latest in teaching and research 2
... offers a dedicated system for SFC and ... series of HPLC instrumentation. JASCO has recently ... capability of the system: ,- The PU-1580-CO2 ... extraction procedures using carbon dioxide as the ...
... trays and universal trays make vessel ... Shaking speed: 40 - ... amplitude Clamps for Erlenmeyer ... including Fernbach flasks Hinged ...
GLP expression profiling service on the Affymetrix platform. Starting material can be blood, tissue or RNA. 5 day turnaround. Standard and custom post analyses of microarray data available....
... The MONOLIGHT 3096 Microplate Luminometer ... chemi- and bioluminescence and for,all measurements ... microplates,i.e. for reactions where the light ... of time, as well as for ...
Biology Products: