Navigation Links
Measuring RNase Activity A Real-time Kinetic Analysis


The RNaseAlert QC System, when used in conjunction with a fluorometer capable of kinetic analysis, provides a rapid and ultra-sensitive assay for determining RNase A specific activity. The RNaseAlert QC System also detects a variety of other nucleases, including RNase A, T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase. In addition to detecting nuclease activity, this kit is useful for standardizing activities of these enzymes.


Sources of RNase

Ribonucleases (RNases) are ubiquitous in the environment, and they are present in relatively high concentrations in many biological materials. For example, the pancreas is rich in RNase (>1 mg RNase/1 g tissue) and is the source for most commercially produced RNase A (and its glycosylated derivative, RNase B).

RNase A is an endonuclease that cleaves 3' of C and U residues. It is commonly used in molecular biology applications such as the removal of contaminating RNA from DNA preparations and ribonuclease protection assays (RPA). Ambion sells an RPA grade RNase A (Cat #2272) that is equivalent to molecular biology grade RNase A from other suppliers, as well as affinity-purified RNase A (Cat #2270 and #2271), which is recommended when the absence of DNase and other nonspecific nuclease activities is essential.


Measuring Specific Activity


'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Measuring multiple apoptosis parameters with the Agilent 2100 bioanalyzer
2. Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation
3. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
4. Measuring siRNA-mediated knockdown of IL-8 mRNA
5. Protector RNase Inhibitor
6. Protector RNase Inhibitor Enhance the Protection of RNA against Degradation
7. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
8. Inducing RNAi with siRNA Cocktails Generated by RNase III
9. Dicer vs. RNase III for Preparation of siRNA Cocktails
10. Combat RNase Contamination in the Lab
11. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Measuring RNase Activity Real time Kinetic Analysis

(Date:5/21/2013)...  The Lindsley F. Kimball Research Institute of the New ... Grand Challenges Explorations winner, an initiative funded by the ... Sara Lustigman , Head of the Laboratory of Molecular ... project, titled Innovative 3-D in vitro culturing ... screen drugs to help eliminate onchocerciasis (river blindness) in ...
(Date:5/21/2013)... study led by George Washington University Professor Tianshu ... ice in small droplets is strongly size-dependent, an ... nanoscale. The formation of ice at the nanoscale ... answer also has important implications for climate research ... ice from supercooled water is generally initiated by ...
(Date:5/21/2013)... Branchburg, NJ (PRWEB) May 21, 2013 ... enhancement industry, has appointed Dale Braddy to Southeastern USA ... proactively bolster its sales muscle in the Southeast. ... a food ingredient brokerage and supplier to food processors ... past 13 years in the food industry and has ...
(Date:5/21/2013)... ALBANY, New York , May 21, 2013 /PRNewswire/ ... published by Transparency Market Research ( http://www.transparencymarketresearch.com ) "Biochip ... Science Research, IVD Tests) - U.S. Industry Analysis, Size, Share, ... USD 2.4 billion in 2011 and is expected to ... 2018, to reach an estimated value of USD 4.7 ...
Breaking Biology Technology:New York Blood Center's Research Institute Receives Grand Challenges Explorations Grant For Groundbreaking Research in Global Health and Development 2New York Blood Center's Research Institute Receives Grand Challenges Explorations Grant For Groundbreaking Research in Global Health and Development 3Study led by GW professor provides better understanding of water's freezing behavior at nanoscale 2The U.S. Biochip Products and Services Market is Expected to Reach USD 4.7 Billion in 2018: Transparency Market Research 2The U.S. Biochip Products and Services Market is Expected to Reach USD 4.7 Billion in 2018: Transparency Market Research 3The U.S. Biochip Products and Services Market is Expected to Reach USD 4.7 Billion in 2018: Transparency Market Research 4The U.S. Biochip Products and Services Market is Expected to Reach USD 4.7 Billion in 2018: Transparency Market Research 5
... University of Idaho have developed unique new technology that ... study the Earth and Sun-Earth connection. , Under ... Yeh, a senior engineer at NASA,s Goddard Space Flight ... University of Idaho,s (U-Idaho) Center for Advanced Microelectronics ...
... Pittsburgh researchers have developed the first natural, nontoxic ... could help diminish the environmental and health concerns ... super-strong materials commonly used in products, from electronics ... found that carbon nanotubes deteriorate when exposed to ...
... Calif., Dec. 17 Once thought of as,a ... graphics,processing units (GPUs) with CUDA(TM) technology are now ... Berkeley,s Open Infrastructure,for Network Computing (BOINC), one ... is using CUDA technology to tap the massively ...
Cached Biology Technology:New one-of-a-kind technology will fly on two NASA missions 2New one-of-a-kind technology will fly on two NASA missions 3Pitt researchers create nontoxic clean-up method for potentially toxic nano materials 2NVIDIA CUDA Technology Dramatically Advances the Pace of Scientific Research 2NVIDIA CUDA Technology Dramatically Advances the Pace of Scientific Research 3NVIDIA CUDA Technology Dramatically Advances the Pace of Scientific Research 4NVIDIA CUDA Technology Dramatically Advances the Pace of Scientific Research 5NVIDIA CUDA Technology Dramatically Advances the Pace of Scientific Research 6
(Date:5/22/2013)... on how climate change will affect interactions between species ... Journal of Animal Ecology . This knowledge, they say, ... of how species are likely to be impacted by ... recognition among biologists that climate change is affecting how ... going to have very important consequences for the stability ...
(Date:5/21/2013)... attached to a whale entangled in fishing gear, scientists ... a whale,s diving and swimming behavior. The monitoring revealed ... migrate, depletes their energy as they drag gear for ... death. , The scientists in this entanglement response suction-cupped ... female North Atlantic right whale called Eg 3911. The ...
(Date:5/21/2013)... new method for delivering molecules into single, targeted ... The technique could find applications in drug delivery, ... electroporation a technique used to deliver molecules ... membrane that are caused by exposing them to ... of cell transfection. (Cell transfection is the introduction ...
Breaking Biology News(10 mins):UCLA life scientists present new insights on climate change and species interactions 2UCLA life scientists present new insights on climate change and species interactions 3UCLA life scientists present new insights on climate change and species interactions 4UCLA life scientists present new insights on climate change and species interactions 5Study reveals how fishing gear can cause slow death of whales 2Study reveals how fishing gear can cause slow death of whales 3Single-cell transfection tool enables added control for biological studies 2
... -- Broccoli, cabbage, leafy greens, caulifloweryour mother insisted they ... your plate. Perhaps you didn,t believe they were all that ... taste better? Turns out your mother was right. These ... your body,s cells. In fact, a recent study by Vanderbilt ...
... This release is available in German . ... and Europe harbour more plant species than rural areas. ... to each other and often share similar functions. Consequently, ... than rural ecosystems. This is concluded by German and ...
... Research in Biomedicine (IRB Barcelona) and the Barcelona Supercomputing Center ... structural information from a triple helix DNA in gas phase, ... practically in a vacuum. The study appears today in the ... of the journals with greatest impact in chemistry. ...
Cached Biology News:The power of broccoli, in a capsule 2The power of broccoli, in a capsule 3Live fast, die young 2First description of a triple DNA helix in a vacuum 2
... provides clear advantages over traditional electrophoresis ... expression), mutation analysis, and size polymorphisms ... microplate connectivity is ideal for basic ... The P/ACE Series automated capillary ...
The imaging screen-K (Kodak), tritium, is a 20 x 25 cm storage phosphor screen that is used for the quantitation and imaging of 32P, 33P, 35S, 14C, and 3H....
The optional fixed lens, 105 mm, is for use with the VersaDoc imaging systems for imaging small samples at high resolution....
The filter 640DF35 for the Molecular Imager FX is a band pass filter for Texas Red dye....
Biology Products: