Navigation Links
Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation

Perform RT-PCR Directly in Cell Lysates Using Cells-to-cDNA II


Quantitative or real time RT-PCR is one of the most commonly used techniques for quantitating changes in gene expression across samples. When working with cell or tissue samples for subsequent use in RT-PCR you must extract RNA, DNase I treat the sample to eliminate contaminating genomic DNA, and then inactivate the DNase I with a Proteinase K treatment and/or a phenol extraction step. If only small numbers of cells (or limited amounts of tissue) are available, there is a risk of losing some of the RNA sample during these steps. Here we describe how Ambion's Cells-to-cDNA II Kit can be used with siRNA transfected cells to measure gene silencing directly in cell lysates, without the need to extract RNA.


Cells-to-cDNA II: Reverse Transcription without RNA Isolation
The RNA isolation step is usually the most laborious and time-consuming part of any RNA analysis protocol, especially when multiple samples need to be analyzed. Ambion's Cells-to-cDNA II Kit (patent pending) offers a significantly more convenient and streamlined procedure to assess gene expression changes in cell samples via RT-PCR. The Cells-to-cDNA II Kit yields cDNA from cultured mammalian cells in less than 2 hours without RNA isolation. The procedure is simple. Cells are lysed in the Cell Lysis Buffer included in the kit, and DNase I treated. cDNA synthesis is then performed directly with this lysate
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Measuring multiple apoptosis parameters with the Agilent 2100 bioanalyzer
2. Measuring RNase Activity A Real-time Kinetic Analysis
3. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
4. Measuring siRNA-mediated knockdown of IL-8 mRNA
5. RNAi Gene Silencing System
6. Quantification of siRNA Silencing Efficiency Using the LightCycler System
7. Drive Long-Term Silencing with CMV Vectors
8. Enhanced siRNA Delivery and Long-term Gene Silencing
9. Fast, Accurate Assessment of siRNA-induced Gene Silencing
10. Superior Gene Silencing Using Adenoviral Vectors
11. Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Measuring Gene Silencing Effects PCR Without RNA Isolation

(Date:7/31/2014)... 31, 2014 Today BioSpace , the leading life sciences ... campaign spotlighting the life sciences community in Illinois , ... Kansas , Michigan , Minnesota ... Ohio and Wisconsin . ... of all job postings on BioSpace originating in this region in 2013. ...
(Date:7/31/2014)... ability to see through organs and even the entire ... as fine-grained cellular structures has been a long-time dream ... Press July 31st in the journal Cell ... methods for making opaque organs, bodies, and human tissue ... intact. The protocols could pave the way for a ...
(Date:7/31/2014)... According to a new market ... - Global Industry Analysis, Size, Share, Growth, Trends and ... USD 88.5 million in 2011, which is expected to ... CAGR of 53.8% from 2013 to 2023. ... Increasing demand for energy efficient displays ...
(Date:7/31/2014)... 2014 According to a new ... Medical Devices Market (Diagnostic Electrodes - ECG, EEG, EMG, ... Pacemakers, Defibrillators, Electrosurgical and Other Electrodes) - Global Industry ... 2019," the global electrodes market for medical devices was ... estimated to reach a market worth USD 1,451.2 million ...
Breaking Biology Technology:Midwest Biotech Leaders Featured On BioSpace Map 2See-through organs and bodies will accelerate biomedical discoveries 2Global Quantum Dots Market is Expected to Reach USD 8,246.8 Million in 2023: Transparency Market Research 2Global Quantum Dots Market is Expected to Reach USD 8,246.8 Million in 2023: Transparency Market Research 3Global Quantum Dots Market is Expected to Reach USD 8,246.8 Million in 2023: Transparency Market Research 4Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 2Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 3Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 4Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 5Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 6
... BHRT ) a company committed to delivering ... treat heart failure and,cardiovascular diseases, announced the adoption ... -- Shareholders approved the election of eight ... in 2009 or until their successors are,duly elected ...
... July 30 DNA Heritage has recently,overcome patent claims ... use of DNA testing and databases that allow families ... DNA. The patent covers the,use of surnames and Y-chromosomes ... (UK IPO) has now rendered a formal opinion,stating that ...
... ROCKVILLE, Md., July 30 Stem cell ... Dr. Thomas Hazel will be,rejoining the company as ... Dr. Karl Johe, Neuralstem,s Chairman and Chief Science,Officer, ... ALS,(Amytrophic Lateral Sclerosis, or Lou Gehrig,s disease), and ...
Cached Biology Technology:Bioheart, Inc. Announces Results of Annual Meeting 2Bioheart, Inc. Announces Results of Annual Meeting 3Bioheart, Inc. Announces Results of Annual Meeting 4Bioheart, Inc. Announces Results of Annual Meeting 5DNA Heritage Success in Patent Battle Helps Keep Genealogy DNA Test Prices Low 2Neuralstem Fills Key Product Development Position 2
(Date:7/31/2014)... bigeye tuna movements in the northwestern Atlantic Ocean led ... Center at the University of Massachusetts Amherst, found among ... range with pronounced north-south movements from Georges Bank to ... off Cape Hatteras southwest of Bermuda for foraging. , ... study one of the most important commercial tuna species ...
(Date:7/31/2014)... Science Foundation (NSF), counters a widely-held scientific view ... instead that certain Arctic lakes store more greenhouse ... , The study, published this week in the ... which occur as permafrost thaws and creates surface ... what was previously frozen land into lakes. , ...
(Date:7/31/2014)... GAINESVILLE, Fla. --- By tracing nearly 3,000 genes ... moths, University of Florida scientists have created an ... to use large-scale, next-generation DNA sequencing. , ... more closely related to small moths than to ... how butterflies evolved. The study also found that ...
Breaking Biology News(10 mins):Study of bigeye tuna in Northwest Atlantic uses new tracking methods 2Study of bigeye tuna in Northwest Atlantic uses new tracking methods 3Certain Arctic lakes store more greenhouse gases than they release 2UF study advances 'DNA revolution,' tells butterflies' evolutionary history 2UF study advances 'DNA revolution,' tells butterflies' evolutionary history 3
... be complete without mosquitoes nipping at exposed skin. Or ... University team may help solve a problem that scientists ... Kun Yan Zhu, professor of entomology, and teammates ... Jianzhen Zhang, a visiting scientist from Shanxi University, China, ...
... Researchers writing in the open access journal BMC ... an adaptation in the blood of Eastern moles which ... moles, burrowing behavior. Kevin Campbell from the ... researchers to study the blood of three underground species ...
... has developed a "lab on a chip" device for ... white blood cells called T cells that are affected ... the test detects the types and levels of inflammatory ... Revzin,s team collaborated with UCLA electrical engineer Prof. Aydogan ...
Cached Biology News:K-State researchers find gene-silencing nanoparticles may put end to pesky summer pest 2Adaptation in mole blood aids tunnelling 2Novel microfluidic HIV test is quick and cheap 2
... Receptor (GR) Competitor Assay Kits are ideal ... compounds. The PolarScreen GR Competitor Assay Kits ... buffer, and one of the following proprietary ... kit), GS Red (red assay kit), or ...
... contains enough separation reagents to ... of 5 x 10 8 ... StemSep red magnet and all ... for the specific cell type ...
... Source rat adult and fetal ... $550.00, * T75 plated cells ... * This product requires Celprogen ... M55007-08 and extracellular matrix, * ...
... Tissue Arrays are intended for laboratory research ... fixed in neutral buffered formalin and embedded ... was performed by certified pathologists. Tissue ... and relocated into a new recipient paraffin ...
Biology Products: