Navigation Links
Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation

Perform RT-PCR Directly in Cell Lysates Using Cells-to-cDNA II


Quantitative or real time RT-PCR is one of the most commonly used techniques for quantitating changes in gene expression across samples. When working with cell or tissue samples for subsequent use in RT-PCR you must extract RNA, DNase I treat the sample to eliminate contaminating genomic DNA, and then inactivate the DNase I with a Proteinase K treatment and/or a phenol extraction step. If only small numbers of cells (or limited amounts of tissue) are available, there is a risk of losing some of the RNA sample during these steps. Here we describe how Ambion's Cells-to-cDNA II Kit can be used with siRNA transfected cells to measure gene silencing directly in cell lysates, without the need to extract RNA.


Cells-to-cDNA II: Reverse Transcription without RNA Isolation
The RNA isolation step is usually the most laborious and time-consuming part of any RNA analysis protocol, especially when multiple samples need to be analyzed. Ambion's Cells-to-cDNA II Kit (patent pending) offers a significantly more convenient and streamlined procedure to assess gene expression changes in cell samples via RT-PCR. The Cells-to-cDNA II Kit yields cDNA from cultured mammalian cells in less than 2 hours without RNA isolation. The procedure is simple. Cells are lysed in the Cell Lysis Buffer included in the kit, and DNase I treated. cDNA synthesis is then performed directly with this lysate
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Measuring multiple apoptosis parameters with the Agilent 2100 bioanalyzer
2. Measuring RNase Activity A Real-time Kinetic Analysis
3. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
4. Measuring siRNA-mediated knockdown of IL-8 mRNA
5. RNAi Gene Silencing System
6. Quantification of siRNA Silencing Efficiency Using the LightCycler System
7. Drive Long-Term Silencing with CMV Vectors
8. Enhanced siRNA Delivery and Long-term Gene Silencing
9. Fast, Accurate Assessment of siRNA-induced Gene Silencing
10. Superior Gene Silencing Using Adenoviral Vectors
11. Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Measuring Gene Silencing Effects PCR Without RNA Isolation

(Date:7/31/2015)... ... July 31, 2015 , ... Global Resorcinol Market 2015-2019 ... insights on the global resorcinol industry. The report highlights the various growth prospects ... scenarios and analysis of primary and secondary inputs from the existing players of ...
(Date:7/30/2015)... ... July 31, 2015 , ... Ralco is honored to announce ... Lyon County Fair to be held August 5-9 in Marshall. The Ralco Enrichment Center ... come from and how agriculture impacts their daily lives. This unique exhibit also features ...
(Date:7/30/2015)... USA (PRWEB) , ... July 30, 2015 , ... ... microsystems, has appointed Francois Vieillard as Sales & Marketing Director. , With ... Francois Vieillard will be responsible for reinforcing Tronics’ business development activities worldwide. He ...
(Date:7/30/2015)... Diego , CA (PRWEB) , ... July 30, ... ... announced the successful completion of a field clinical study of its canine osteoarthritis ... developed together with Aratana Therapeutics (Kansas City, KS) and will be marketed in ...
Breaking Biology Technology:Resorcinol Market to Grow at 6% CAGR to 2019 Says New Research Report at ReportsnReports.com 2Resorcinol Market to Grow at 6% CAGR to 2019 Says New Research Report at ReportsnReports.com 3Resorcinol Market to Grow at 6% CAGR to 2019 Says New Research Report at ReportsnReports.com 4Ralco Announces Primary Sponsorship of Ralco Enrichment Center at Lyon County Fair 2Tronics appoints Francois Vieillard as Sales and Marketing Director 2VetStem Biopharma Successfully Completes Large Milestone Efficacy Study for its Flagship Canine Osteoarthritis Stem Cell Product 2VetStem Biopharma Successfully Completes Large Milestone Efficacy Study for its Flagship Canine Osteoarthritis Stem Cell Product 3
... Corp ., a Madison biotechnology company, has raised $1 million ... the total raised to $3 million in the past two ... verify the efficacy of two products: adhesives that bind tissues ... and medical device coatings that block bacterial adhesion to the ...
... - Sir Ken Robinson has many ... his point about creativity than the story of a little ... a senior advisor to the J. Paul Getty Trust ... creativity and innovation, told the story during a keynote address ...
... 1995, I gave a luncheon speech to the Chicago Bar ... st Century." At that time, many who attended ... that much faith in it. My argument was that the ... maxed out. Municipalities would have to become more creative to ...
Cached Biology Technology:Sir Ken Robinson: Companies, kids "get" innovation, but K-12 doesn't nurture it 2Sir Ken Robinson: Companies, kids "get" innovation, but K-12 doesn't nurture it 3Creativity, innovation extend to municipalities 2Creativity, innovation extend to municipalities 3Creativity, innovation extend to municipalities 4
(Date:7/31/2015)... 31. juli 2015 Den 10. internationale konference ... BGI fra den 22. - 25. oktober i ... sin 10-års fødselsdag i år. Siden starten i 2006 ... møder på ,omik,-feltet, og er en af de mest ... ICG-10 fokuserer på nylige gennembrud og fremskridt inden for ...
(Date:7/31/2015)... , 31 de julio de 2015 BGI llevará ... www.icg-10.org ) del 22 al 25 de octubre de ... La conferencia celebra su décimo aniversario este año. ... en una de las reuniones anuales más influyentes del ... una de las conferencias científicas más dinámicas, entusiastas y ...
(Date:7/31/2015)... 31 de julho de 2015 A 10 ... ) será realizada pela BGI de 22-25 de outubro de ... A conferência está celebrando seu 10 o. ... ICG se tornou uma das reuniões mais influentes do ... científicos mais dinâmicos, entusiastas e prazerosos. ...
Breaking Biology News(10 mins):Den 10. internationale konference om Genomics (ICG-10) åbner i oktober 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 3A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 2A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 3
... is speeding up the mortality of trees, and Northern Arizona ... it. Pete Ful, an NAU associate professor in the ... Restoration Institute, is a coauthor of "Widespread Increase of Tree ... be published in the Jan. 23 issue of Science ...
... Significant loss of life, destroyed property and businesses, ... replacing Federal Emergency Management Agency flood maps with ... elevation data, says a new report from the ... accurate flood maps will outweigh the costs, mainly ...
... side, then eating less may help you live longer. ... authors of a new study predict the anti-aging strategy ... even dangerous exercise. "Today there are a lot of ... into this," said Raj Sohal, professor at the University of ...
Cached Biology News:NAU research ties tree mortality trends to climate warming 2More accurate FEMA flood maps could help avoid significant damages and losses 2More accurate FEMA flood maps could help avoid significant damages and losses 3More accurate FEMA flood maps could help avoid significant damages and losses 4Eating less may not extend life 2
... sensitivity of DNA detection has been improved. With ... agarose gel can be detected > 20 ng. ... purified by organic solvent extraction or with other ... well.) DNA excised from agarose gel can ...
Mouse monoclonal antibody raised against a full length recombinant CSNK2A2. NCBI Entrez Gene ID = CSNK2A2...
... Rockers, mini, SSM3 & SSM4, Small space saving ... action , Choice of two models: , - ... SSM4 , Digital speed control and built-in ... ,These compact rockers are ideal where gentle mixing ...
... TargeTron(TM) Gene Knockout System provides optimized reagents ... disruption of bacterial genes by insertion of ... retrohoming ability of group II introns and ... the TargeTron group II intron for specific ...
Biology Products: