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Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)

oplate spectrofluorometer. With its dual scanning monochromators, it easy to optimize excitation and emission wavelengths for specific fluorophores and to customize the settings for combinations of probes. Mixtures of 2, 3, and (probably) 4 probes in a single mixture can be resolved.


REFERENCES
1. Tyagi, S. and F.R. Kramer. (1996) Molecular Beacons: Probes that Fluoresce upon Hybridization. Nature Biotechnology 14: 303308.

2. Tyagi, S., D.P. Bratu and F.R. Kramer. (1998) Multicolor molecular beacons for allele discrimination. Nature Biotechnology 16: 4953.

3. Marras, S.A.E., F.R. Kramer and S. Tyagi. (1999) Multiplex detection of single-nucleotide variations using molecular beacons. Genetic Analysis: Biomolecular Engineering 14: 151156.

4. Bonnet, G, S. Tyagi, A. Libchaber and F.R. Kramer. (1999) Thermodynamic basis of the enhanced specificity of structured DNA probes. Proc. Nat. Acad. Sci. 96, 61716176.

5. McGown, E.L. (1999) Selecting excitation and emission wavelengths using the SPECTRAmax GEMINI microplate spectrofluorometer basic principles. MAXline Application Note No. 30.

6. McGown, E.L. (1999) Optimizing excitation and emission wavelengths for narrow Stokes shift fluorophores using the SPECTRAmax GEMINI microplate spectrofluorometer. MAXline Application Note No. 31.


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