Navigation Links
Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)

ere Nunc brand, black 384well microplates, surface-treated to minimize binding (cell culture-treated). Allsamples were prepared in 10 mM Tris buffer, pH 8, containing 1 mM MgCl2, and the final concentrations of beacon and target were 0.3 M and 1.6 M respectively, in a total volume of 54 L. The reaction mixtures were incubated at least 30 minutes at ambient temperature before measurement in a SPECTRAmax GEMINI microplate spectrofluorometer.


RESULTS AND DISCUSSION
Figure 1 shows the fluorescence spectrum of a tetrachlorofluorescein (TET)labelled probe in the presence and absence of complementary target. At the peak maximum (approximately 535 nm), the probe/target complex had approximately 35 times higher signal than did the probe alone.


The optimal wavelength settings for TET and several other molecular beacons were determined by examination of signal/blank ratios as previously described.5,6 The combination of excitation/emission wavelengths and emission cutoff filters giving the highest ratios are summarized in the table below.


There is considerable interest in measuring multiple targets (multiplexing) in a single sample. This can be done by using a different fluorophore for each probe, assuming the probes do not interfere with each other. If their emission spectra do not overlap, detection of one in the presence of the other is straightforward. Such is the case for fluorescein (FAM)- and tetramethylrhodamine (TAMRA)-labelled probes. Using the instrument settings intended for FAM, the TAMRA probe/ target complex does not interfere with the FAM signal ( Figure 2 ).


Conversely, when TAMRA settings are used, the FAM probe/target complex does not interfere with the TAMRA signal (Figure 3). There are many other 2-probe combinations which would be compatible. If two probes have partially overlapping em
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
2. Cell Proliferation and Viability Measurement
3. LabVIEW for Measurement and Data Analysis
4. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
5. Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor
6. In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate
7. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
8. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
9. PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)
10. UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)
11. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
Post Your Comments:
(Date:1/23/2015)... January 23, 2015 Portal Solutions , ... workplaces on Microsoft Office 365 and SharePoint platforms , ... Award program for being one of the region’s fastest-growing mid-sized ... employee growth. , “To be recognized a second year ...
(Date:1/22/2015)... , Jan. 22, 2015  Transwestern | RBJ today announces the ... office space for Shire a leading biopharmaceutical company, at Two ... | RBJ,s Robert Richards , president, and Brian ... for the entire five-floor building at 95 Hayden Ave. ...
(Date:1/22/2015)... 22, 2015 Pipette.com has added the ... their comprehensive portfolio of Eppendorf products. , The Eppendorf ... a Centrifuge 5424/5424 R and receive the following:, ... Research plus or Eppendorf Reference 2 , ...
(Date:1/22/2015)... NJ (PRWEB) January 22, 2015 ... technologies such as the Bioruptor® and complete solutions ... system for chromatin immunoprecipitation, alleviating the need for ... and controls needed for ChIP of histones or ...
Breaking Biology Technology:Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 2Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 3Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2
... 13 ADVENTRX Pharmaceuticals,Inc. (Amex: ANX ... on commercializing proprietary product candidates for the ... that Evan M. Levine,ADVENTRX,s chief executive officer, ... Fifth Annual Growth Conference on Wednesday,September 19th, ...
... ... to inhibit ... 13, 9:45 a.m. EDT at ... http://www.isispharm.com , CARLSBAD, Calif., Sept. 13 Isis,Pharmaceuticals, Inc. (Nasdaq: ISIS ...
... VANCOUVER, Sept. 13 /PRNewswire-FirstCall/ - Angiotech Pharmaceuticals,Inc. ... today that it received a,favourable decision from ... an attempt by Johnson & Johnson,s subsidiary, ... Zealand Patent 523799, which,pertains to compositions comprising ...
Cached Biology Technology:ADVENTRX Pharmaceuticals to Present at the ThinkEquity Partners' Fifth Annual Growth Conference 2Isis Enters Broad Collaboration With Ortho-McNeil, Inc. for the Discovery, Development and Commercialization of Antisense Drugs to Treat Metabolic Diseases 2Isis Enters Broad Collaboration With Ortho-McNeil, Inc. for the Discovery, Development and Commercialization of Antisense Drugs to Treat Metabolic Diseases 3Isis Enters Broad Collaboration With Ortho-McNeil, Inc. for the Discovery, Development and Commercialization of Antisense Drugs to Treat Metabolic Diseases 4Isis Enters Broad Collaboration With Ortho-McNeil, Inc. for the Discovery, Development and Commercialization of Antisense Drugs to Treat Metabolic Diseases 5Angiotech receives favourable patent decision from New Zealand's Intellectual Property Office 2Angiotech receives favourable patent decision from New Zealand's Intellectual Property Office 3Angiotech receives favourable patent decision from New Zealand's Intellectual Property Office 4
(Date:12/17/2014)... 2014 The Defense Logistics Agency is insourcing its ... counterfeit microcircuits from entering into its supply chain. ... anti-counterfeit initiative dubbed DNA marking. The capability will validate ... throughout the supply chain. The new quality control measures ...
(Date:12/17/2014)... Valencell, a leader in performance biometric data sensor technology, ... such as Intel, Jabra, Atlas, and Scosche to develop ... products will be showcased at CES 2015, taking place ... "Our partners choose PerformTek biometrics, because ...
(Date:12/17/2014)... is fundamentally transforming the travel experience and nowhere is ... past decade, ePassports, biometric readers, and secure document scanners ... control via eGates and Automated Passport Control (APC) Kiosks ... borders across the globe. According to ...
Breaking Biology News(10 mins):Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 2Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 3Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Automated Border Control (ABC) Transforms the Global Travel Experience With More Than 2500 ABC eGates and APC Kiosks Deployed In Airports, Seaports, and Land Borders Worldwide 2
... in the international conservation journal Oryx ... reintroduction of captive lions do little to further the conservation ... Online, on July 31, ,Walking with lions: Why there is ... species restoration, was authored by a blue-ribbon panel of lion ...
... cancer from one tissue to another may one day be ... print issue of The FASEB Journal ( http://www.fasebj.org ... on fruit flies) increases the "stickiness" that causes cells, including ... slows their movement throughout the body. "The study of ...
... the body are injured at their farthest reaches, coordinating the ... because these peripheral nerve cells can be extremely long ... a lot of distance for a molecular distress signal to ... cell,s nucleus. Scientists have believed this process to be ...
Cached Biology News:Report: Captive lion reintroduction programs in Africa operate under 'conservation myth' 2Report: Captive lion reintroduction programs in Africa operate under 'conservation myth' 3Long-distance distress signal from periphery of injured nerve cells begins with locally made protein 2Long-distance distress signal from periphery of injured nerve cells begins with locally made protein 3
... Todays advanced protein cross-linking techniques are defined ... Dr. Shan S. Wong. This valuable hardcover ... schemes and current application references. More than ... protein cross-linking and conjugate preparation from ...
... active ingredient in Molecular Probes. SlowFadeAntifade ... to act as a free radical ... Our original SlowFade formulation (S-2828) was ... of fluorescein to almost zero (Figure ...
b6-2A12...
... kit are prepared with pure chemicals according ... Isolation Kit is designed to isolating intact ... easy way. One kit is consisted of ... mitochondria from 10 20 g tissue ...
Biology Products: