Navigation Links
Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)

Evelyn McGown and Michael Su
October 1999


Molecular beacons are fluorogenic oligonucleotide probes developed by Kramerand associates to detect specific nucleic acids in homogeneous solutions.1 4 They are single-stranded oligonucleotides that exist in a hairpin shape because of thecomplementary arms at the ends which bind together to form a stem. Thestrained loop portion contains the nucleotide that is complementary to theintended target. A fluorophore is covalently bound to one end of theoligonucleotide and a quencher to the other end. In the absence of target, thefluorophore and quencher are held in close proximity by the arms and thefluorescence is internally quenched. When the probe hybridizes to the targetmolecule, it undergoes a conformational change resulting in separation of thefluorophore and quencher, and restoration of fluorescence. Molecular beacons areappealing because they can be used in homogeneous solutions and obviate theneed to isolate probe/target hybrids from an excess of unbound hybridizationprobes. They have been used to monitor polymerase chain reactions.3

Many combinations of fluorophores and quenchers are possible. Kramers grouphas found that 4-(4-dimethylaminophenylazo) benzoic acid (DABCYL) can serveas a universal quencher for a number of fluorophores. Even if the fluorophoreemission spectrum does not overlap with the DABCYL absorption spectrum,quenching (with >95% efficiency) occurs by direct energy transfer fromfluorophore to quencher because of their close proximity.2

This application note describes measurement of probe/target complexes with theSPECTRAmax GEMINI microplate spectrofluorometer.


MATERIALS AND METHODS
Samples of molecular beacons and complementary targets were obtained fromResearch Genetics, Huntsville, Alabama. The oligonucleotide sequences are notimportant to this discussion. The microplates used w
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
2. Cell Proliferation and Viability Measurement
3. LabVIEW for Measurement and Data Analysis
4. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
5. Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor
6. In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate
7. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
8. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
9. PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)
10. UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)
11. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
Post Your Comments:
(Date:12/19/2014)... MA (PRWEB) December 18, 2014 iLab ... announced the appointment of Siri Bryant as the new ... new Director of Core Implementations. These two new leadership ... outstanding customer service by ensuring that iLab continues to ... “In the last four years, iLab has been deployed ...
(Date:12/19/2014)... BURLINGTON, Mass. , Dec. 18, 2014  Decision ... dental biomaterial market will grow moderately through ... implant procedures and growing awareness of dental biomaterials all ... underdeveloped markets such as China ... incomes will bring dental procedures within reach for a ...
(Date:12/17/2014)... (PRWEB) December 16, 2014 The ... since 2013, which is why IBISWorld updated its original ... continues to benefit from an intensified focus on environmental ... for new emission standards for power plants and a ... According to IBISWorld Industry Analyst Sarah Kahn, “a range ...
(Date:12/17/2014)... N.J. (PRWEB) December 17, 2014 ... (Euronext: IPN; ADR: IPSEY), today announced ... (referred to as Somatuline®) was approved by the ... treatment of gastroenteropancreatic neuroendocrine tumors (GEP-NETs) in adult ... advanced or metastatic disease to improve progression-free survival ...
Breaking Biology Technology:iLab Solutions Announces Two New Leadership Positions to Meet the Needs of Its Growing Customer Base 2Asia Pacific Dental Biomaterial Market Will Grow Strongly Through 2023 2Asia Pacific Dental Biomaterial Market Will Grow Strongly Through 2023 3Activated Carbon Manufacturing in the US Industry Market Research Report Has Been Updated from IBISWorld 2Activated Carbon Manufacturing in the US Industry Market Research Report Has Been Updated from IBISWorld 3Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 2Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 3Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 4Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 5Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 6Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 7Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 8
... Calif., Nov. 4, 2010 Onda Corporation, the global ... markets, introduced the latest additions to the hydrophone product ... The HFO-660 is a new fiber optic hydrophone system ... for emerging applications of HITU. It is complemented with ...
... EDISON, N.J., Nov. 4, 2010 International Technidyne Corporation ... THOR ), announced today the completion of its ... private equity firm, in an all cash transaction valued ... leader in hemostasis management and Point-of-Care (POC) testing for ...
... HOUSTON ― An antibody loaded with an anti-cancer agent produced ... relapsed or therapy-resistant Hodgkin lymphoma enrolled in a phase I ... the New England Journal of Medicine . "That ... a Phase I trial," said study lead author Anas Younes, ...
Cached Biology Technology:Onda Introduces a New Fiber Optic Hydrophone and Scanning Tank 2International Technidyne Corporation Acquired by Warburg Pincus 2International Technidyne Corporation Acquired by Warburg Pincus 3Armed antibody triggers remissions for Hodgkin's lymphoma 2Armed antibody triggers remissions for Hodgkin's lymphoma 3
(Date:12/10/2014)... 2014  Wake Forest Baptist Medical Center today announced plans ... of Medicine. Funding for this $50 million capital project ... be publicly launched next summer. The ... 60 series R.J. Reynolds Tobacco Company complex, adjacent to ... immediately with plans to be ready to welcome medical ...
(Date:12/5/2014)... Calif. , Dec. 4, 2014 ... has boosted investments in new testing and inspection ... also given rise to a range of innovative ... the requirements of Generation Y, which is generally ... As a result, product development strategies of test ...
(Date:12/3/2014)... -- As part of our commitment to offering the ... announce the release of a new reader that will ... data that they need. The Atlas 2000 ... readers. Many such devices have serious shortcomings when it ... models force users to navigate numerous complicated steps in ...
Breaking Biology News(10 mins):Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 2Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 3Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 4Inception Technologies to Release New Biometric Reader 2
... PROVIDENCE, R.I. [Brown University] It,s been long known ... cells stabbed with spiky, long asbestos fibers, and the image ... cell, like a quivering arrow that,s found its mark. ... be interested in asbestos fibers and other materials at the ...
... ITHACA, N.Y. With an eye toward understanding DNA ... to actually unzip the two strands of DNA. (Nature, online ... processes, including DNA replication, are enzymes called helicases. Acting like ... prompting the strands to "zip" apart. What had been ...
... already led to major shifts in the abundance of commercially ... on September 15 in Current Biology , a Cell ... abundance of species as opposed to their presence or absence ... well on the European shelf than more northerly, cold-adapted species," ...
Cached Biology News:Why carbon nanotubes spell trouble for cells 2For unzipping DNA mysteries -- literally -- Cornell physicists discover how a vital enzyme works 2In rapidly warming seas, some fish lose while others gain 2
Immunogen: Synthetic peptide: N(171) H G F L S A D Q QL I K(183) Storage: -20 C, Avoid Freeze/Thaw Cycles...
The pCDF Expression System 1 plus Competent Cells contains 10 µg of pET-45b(+) DNA. pET-45b carries an N-terminal His•Tag ® coding sequence that is cleavable with enterokinase as well...
HIV-1 TAT (vN-21)...
Request Info...
Biology Products: