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Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)

rates7 or measurement of homologous DNA recombination repair which plays a role in cell viability/genomic stability8.


ACKNOWLEDGEMENTS
We would like to thank Dr. Georg Kienzle of the Paul Bucher Company, Basel, Switzerland and Dr. Anne T. Ferguson of Molecular Devices Corporation for their valuable input and comments.


PATENT NOTE
Products containing DNA sequences coding for mutant Aequorea victoria green fluorescent protein (GFP) variants or proteins thereof require a license from Aurora Biosciences Corporation under U.S. Patent Nos. 5,625,048, 5,777,079, and 5,804,387 and other pending U.S. and foreign patent applications. For-Profit research institutes or entities that wish to use this product in non-commercial or commercial applications are required to obtain a license from Aurora Biosciences Corporation.


REFERENCES

1. Morise, H., Shimomura, O., Johnson, F.H. & Winant, J. (1974). Intermolecular energy transfer in the bioluminescent system of Aequorea. Biochemistry, 13(12): 26562662.

2. Milligan, G. (1999). Exploring the dynamics of regulation of G protein-coupled receptors using green fluorescent protein. Br. J. Pharmacol., 128: 501510.

3. Cubitt, A.B., Heim, R., Adams, S.R., Boyd, A.E., Gross, L.A. & Tsien RY. (1995). Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci., 20(11): 448455.

4. SPECTRAmax GEMINI XS product literature

5. McGown, E.L. (1999). Selecting Excitation and Emission Wavelengths Using the SPECTRAmax GEMINI Microplate Spectrofluorometer Basic Principles. MAXline Application Note 30.

6. Strebel, A., Harr, T., Bachmann, F., Wernli, M. & Erb, P. (2001). Green fluorescent protein as a novel tool to meas
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