Maximize Viral RNA Yield from Biological Fluid ( Linear Recovery Down to 50 Copie...)

Maximize Viral RNA Yield from Biological Fluid

Linear Recovery Down to 50 Copies of Transcript
With the MagMAX Viral RNA Isolation Kit, viral RNA can be quantitatively recovered from biological fluids spiked with RNA input levels ranging over 5 orders of magnitude. This was demonstrated by spiking plasma, serum, milk, and water samples with dilutions of a 466 nt HIV Armored RNA transcriptan RNase resistant, viral protein-coated RNA molecule. A titration from 50 to 4,000,000 copies of Armored RNA was added to duplicate samples for amplification. The RNA was then recovered from the sample using the MagMAX Kit, and 20% of the sample was used in qRT-PCR to detect the HIV RNA. Figure 3 shows that RNA recovery was linear over a broad range of viral concentrations, i.e. for both low and high viral titers, including samples containing as few as 50 input copies of transcript.

Figure 3. Linear Recovery of Synthetic Viral RNA from Biological Fluids. MagMAX Lysis Buffer was added to samples of water, plasma, serum, and milk, and the mixture was then spiked with a serial dilution of a 466 nt HIV Armored RNA transcript. (Armored RNA is protected from nucleases by a protein coat derived from the MS2 coat protein gene.) Viral RNA was isolated using the MagMAX-96 Viral RNA Isolation Kit. The recovered RNA was analyzed by qRT-PCR. The results demonstrate that as few as 50 input copies of transcript can be successfully detected in RNA isolated using the MagMAX Kit.

Processing Individual Samples
The MagMAX Viral RNA Isolation Kit is ideal for proficiency
'"/>

Source:

Page: All 1 2 3 4 5 6 7
Related biology technology :

1. ArrayScript RT: Maximize Yields of Full-length cDNA
2. Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A
3. Versatile Reporter Vectors for Monitoring Viral Transduction
4. Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun
5. Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus
6. Isolation and Characterization of Anti-Viral Nucleoside Compounds from the Chinese Herb Taraxacum Mongolicum
7. Yeast Protein Production System Features High Yields and One-Step Purification
8. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
9. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
10. Increase Protein Activity and Yield
11. High Yield Transcription for Every Application

Post Your Comments:

( Linear Recovery Down to 50 Copie...)Maximize Viral RNA Yield from Biological Fluid