Navigation Links
Maximize Viral RNA Yield from Biological Fluid

several times, and the RNA is eluted in 20 l of nuclease-free water or low salt buffer. The procedure does not include time-consuming and difficult-to-automate protease digestion, organic extraction, centrifugation, or vacuum filtration steps. The RNA obtained with the kit can be used directly for qRT-PCR and RNA amplification, Northern analysis, and other applications.


Recover a Broad Size Range of RNA from Diverse Samples
To demonstrate the ability of the MagMAX Kit to recover a broad range of differently sized RNAs from diverse samples, lysis solution was added to samples (using water as a positive control). A mixture of eight RNA transcripts ranging in size from 100 nt to 9000 nt were spiked into the mixture. The RNA transcripts were isolated from the samples using the MagMAX Viral RNA Isolation Kit and run on an agarose gel (Figure 2). The MagMAX Kit recovered each of the eight RNA transcripts largely intacteven from raw milk and serum, which have a notoriously high nuclease content.

Figure 2. Efficient Recovery of RNA from Various Sample Types. To test recovery of RNAs ranging in size from 100 bases to 9 kb, MagMAX Lysis Buffer and 250 ng of RNA transcripts of the indicated sizes were added. The samples were then processed using the MagMAX Viral RNA Isolation Kit. The RNA obtained was analyzed on a 1% denaturing agarose gel stained with SYBR Gold. High quality RNA was effectively recovered over a large size range from common sample sources. BHI: brain heart infusion broth. VTM: viral transport media.


'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. ArrayScript RT: Maximize Yields of Full-length cDNA
2. Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A
3. Versatile Reporter Vectors for Monitoring Viral Transduction
4. Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun
5. Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus
6. Isolation and Characterization of Anti-Viral Nucleoside Compounds from the Chinese Herb Taraxacum Mongolicum
7. Yeast Protein Production System Features High Yields and One-Step Purification
8. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
9. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
10. Increase Protein Activity and Yield
11. High Yield Transcription for Every Application
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Maximize Viral RNA Yield from Biological Fluid

(Date:12/14/2009)... a US-based thermoforming company, has completed the delivery of Brookfield ... metal to a plastic enclosure with a smaller footprint and ... ... MA (PRWEB) December 14, 2009 -- ThermoFab, a US-based thermoforming ... Tester – helping Brookfield move from sheet metal to a ...
(Date:12/11/2009)... EntreMed, Inc. (Nasdaq: ENMD ), today announced ... from sales of Thalomid® during the third quarter ... In addition, the Company anticipates a decrease ... 2009. The difference in estimated and actual ... in the fourth quarter financial results. ,, (Logo: ...
(Date:12/11/2009)... BOULDER, Colo., December 10, 2009 miRagen Therapeutics, ... lives by developing innovative microRNA-based therapeutics for cardiovascular ... data demonstrating that microRNA-206 (miR-206) plays a crucial ... (ALS or Lou Gehrig,s disease) and in neuromuscular ... in the December 11 issue of the journal ...
(Date:12/11/2009)... (12/11/2009) Boston College researchers have observed the "hot ... time and successfully harvested the elusive charges using ultra-thin ... power efficiency, the authors report in the current online ... light is captured in solar cells, it generates free ... order to snare these charges, the electrons must reach ...
Breaking Biology Technology:Thermofab Helps Brookfield Engineering Move To A Compact Custom Plastic Enclosure 2EntreMed Reports Receipt of Third Quarter Royalty Payment 2miRagen announces discovery of key microRNA implicated in Lou Gehrig's disease 2Elusive 'hot' electrons captured in ultra-thin solar cells 2
... interesting lesson. , ,In 1970, Dr. Winston Royce ... Large Software Systems. Appearing in Proceedings, IEEE Wescon, the ... would plague the software industry for decades to come. ... make the great mistake. , , Birth of waterfall ...
... - Having survived two rounds of judging, 26 entries from ... fourth annual Wisconsin Governor's Business Plan Contest . , ... nearly 300 entries statewide, and they represent some of the ... Wisconsin Technology Council . , ,The 26 remaining plans include ...
... Wis. - Phillips Plastics Corp. , a ... Prescott, Wis., has entered into a collaborative agreement with ... stem cell therapeutics for orthopedic applications. , ,The agreement ... drug discovery and therapeutic research, represents a convergence of ...
Other Biology Technology:Are you doomed to repeat the great software mistake? 2Are you doomed to repeat the great software mistake? 3Are you doomed to repeat the great software mistake? 4Business plan finalists capture technology 2Phillips Plastics to help develop orthopedics with stem cells 2Phillips Plastics to help develop orthopedics with stem cells 3
(Date:12/11/2009)... has granted an 18-month, exclusive option to startup ... analogs. The company must reach certain milestones in ... exclusive license on therapies designed to help patients ... treatments that it hopes will become an important ... market. Insulin is a therapeutic protein used to ...
(Date:12/11/2009)... Arno Motulsky, the senior editor of the 4th edition ... Human Genetics, was recently given the Victor A. McKusick ... contributions to the field of human genetics, was presented ... American Society of Human Genetics on 24 October 2009 ... Medicine and Genome Sciences at the University of Washington ...
(Date:12/11/2009)... common knowledge that smoking is a health risk but ... than adults? In an evaluation for Faculty of 1000 ... prone to substance abuse. , Grunberg describes the study, ... journal, as "fascinating" and suggests it "may have implications ... drug abuse". , Nicotine increases the level of dopamine ...
Breaking Biology News(10 mins):Case Western Reserve University grants option to startup Thermalin Diabetes Inc. 2iCRco Makes Inroads in Maritime Healthcare and Completes the First Two Installs on World Renowned Cruise Lines 55360 1iCRco Makes Inroads in Maritime Healthcare and Completes the First Two Installs on World Renowned Cruise Lines 55360 2iCRco Makes Inroads in Maritime Healthcare and Completes the First Two Installs on World Renowned Cruise Lines 55360 3Headsets Direct Blog Becomes Real Time Help Desk for Plantronics Headset Questions 55357 1Headsets Direct Blog Becomes Real Time Help Desk for Plantronics Headset Questions 55357 2Headsets Direct Blog Becomes Real Time Help Desk for Plantronics Headset Questions 55357 3Frost 26 Sullivan 3A Growing Healthcare Tourism in Malaysia 55354 1Frost 26 Sullivan 3A Growing Healthcare Tourism in Malaysia 55354 2Frost 26 Sullivan 3A Growing Healthcare Tourism in Malaysia 55354 3
... The special issue (Volume 37, Number 6) of ... first report on all documents related to environment and ... UN General Assembly (UN/GA). During the session at UN ... to many of the worlds most pressing problems was ...
... leader in recent research on the interaction of ... illness, Avshalom Caspi, Ph.D. of King,s College London, ... Prize for Distinguished Achievement in Developmental Psychobiology. Presented ... at Columbia University Medical Center and the Sackler ...
... powerful new tool in the fight against deficiencies in ... in 40 million children annually, and increased health risks ... , This tool consists of "a new method ... enable developing countries to identify and increase cultivation of ...
Other Biology News:World-leading journal publishes special issue on UN/GA 2World-leading journal publishes special issue on UN/GA 3Research pioneer in the developmental origins of psychiatric illness is awarded the Sackler prize 2New way to produce high-vitamin corn could improve nutrition in developing countries 2New way to produce high-vitamin corn could improve nutrition in developing countries 3
Request Info...
... is a preparation of deoxyribonuclease I ... to produce 3-hydroxyl oligonucleotides. This preparation ... where maintaining the integrity of RNA ... for use with the Core Footprinting ...
... the template-directed polymerization of nucleotides into duplex ... I possesses a 3?5 exonuclease activity or ... during DNA replication, and also contains a ... to replace nucleotides in the growing strand ...
RNase-Free DNase 50 l...
Biology Products: