( . RNA was isolated from 50 l of sample ...)Maximize Viral RNA Yield from Biological Fluid

. RNA was isolated from 50 l of sample using the MagMAX protocol or from 500 l of sample using a competitor's product. Even with 10-fold less sample, viral RNA detection in RNA isolated with the MagMAX protocol was as sensitive or more sensitive than from RNA isolated using the competitors kit.

Advantages of Magnetic Beads for Viral RNA Isolation
Magnetic bead-based technology is extremely effective for viral RNA isolation from cell-free samples. The MagMAX technology results in better RNA capture and higher RNA yield than glass filter based techniques. Furthermore, RNA yields are also more consistent, both from experiment to experiment and over a broad range of sample sizeseven from high volume and/or low viral titer samples. Another important benefit of magnetic bead-based RNA capture over glass filter-based methods is that the problem of filter clogging with samples such as plasma and milk is eliminated. Furthermore, since only a small volume of magnetic beads is needed, bound RNA can be eluted in just 20 l of nuclease-free water or low salt buffer. This makes the kit extremely useful for concentrating viral RNA from large, dilute samples.

A Fast and Simple Processing Method
In the MagMAX procedure, plasma, serum, swab samples, or cell free samples undergo a lysis/binding step to denature proteins in the sample and to simultaneously bind the RNA on magnetic beads. The beads are then captured and washed
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