( we sought the assistance of the Nati...)Maximize Viral RNA Yield from Biological Fluid

>, we sought the assistance of the National Veterinary Services Laboraties (NVSL), a subsidiary of the Animal and Plant Health Inspection Service (a division of the USDA). NVSL is charged with providing animal disease testing for Veterinary Services and testing of domestic and foreign diagnostic specimens for animal diseases. Currently NVSL uses another manufacturers RNA isolation product to obtain RNA from biological fluids for their validated viral testing process. When they compared this validated system to Ambions MagMAX Viral RNA Isolation Kit, they found that they could get similar, and often better results using 10-fold less sample with the MagMAX Kit. The experiment shown in Figure 1 is a comparison of C_t values in a real-time qRT-PCR assay for the Exotic Newcastle Disease (END) virus. Cloacal or tracheal swab samples were collected from poultry infected with the virus, and RNA was isolated from 500 l of sample using the current validated RNA isolation product (competitor), or from only 50 l of sample using the MagMAX Viral RNA Isolation Kit (Ambion). Equal volumes of the RNA recovered were used for the viral RNA detection assay. Despite using ten-fold less sample input, the MagMAX Kit produced enough RNA to allow equal or better detection of viral RNA in 9 of the 12 samples.

Figure 1. MagMAX Achieves Better Sensitivity with 10X Less Sample than Competitors Kit. The sensitivity of viral RNA detection using Ambions MagMax Kit was compared with that of the current NVSL-validated viral testing protocol (competitor). Clinical tracheal and cloacal swab samples were collected from poultry infected with California exotic newcastle disease (END) virus
'"/>

Source:

Page: All 1 2 3 4 5 6 7
Related biology technology :

1. ArrayScript RT: Maximize Yields of Full-length cDNA
2. Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A
3. Versatile Reporter Vectors for Monitoring Viral Transduction
4. Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun
5. Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus
6. Isolation and Characterization of Anti-Viral Nucleoside Compounds from the Chinese Herb Taraxacum Mongolicum
7. Yeast Protein Production System Features High Yields and One-Step Purification
8. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
9. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
10. Increase Protein Activity and Yield
11. High Yield Transcription for Every Application