Navigation Links
Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success

igures 3 and 4).

The Silencer GAPDH siRNA Control--and the TaqMan Gene Expression Assay for GAPDH--provide the ideal control siRNA and assay for optimization of transfection, respectively, (Figure 4).


Figure 3. Overview of the Process for siRNA Transfection Optimization.

Figure 4. Using qRT-PCR to Optimize Transfection of siRNA. Cos-7 cells were transfected with CDK2 siRNA or a negative control siRNA using the indicated volumes of transfection agent per well. 48 hr after transfection, the cells were harvested and analyzed by real-time RT-PCR using TaqMan Gene Expression Assays for CDK2 and 18S rRNA. Data from the 18S rRNA reactions were used to normalize input RNA, and the percent CDK2 expression was calculated as the amount of gene expression compared to the negative control siRNA.


Correlating Phenotype with the Extent of Knockdown
To better evaluate functional siRNA experimental results, the phenotype elicited should be correlated with the extent of knockdown induced by a particular siRNA. For a complete picture, both target mRNA levels and corresponding protein levels should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Silencer Validated siRNAs
2. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
3. Five Ways to Produce siRNAs
4. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
5. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Deliver siRNAs Into Primary Cells
9. Optimize Transfection of siRNAs for RNAi
10. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
11. Test More siRNAs for Less
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Matched siRNAs and Assays Ambion Applied Biosystems RNAi Success

(Date:4/16/2015)... Proove Biosciences , Inc. is pleased to ... for the 2015 BIO International Convention. Hosted by the ... will take place June 15-18 at the Pennsylvania Convention ... contest, industry leaders will be voting for companies they ... community voting period began on April 14, 2015 and ...
(Date:4/16/2015)... According to the 2014 Liberty Mutual ... was ranked first among the leading causes of ... pushing, pulling, holding, carrying, or throwing and cost ... Round Company takes statistics like this very seriously. ... develop and design equipment that prevents these injuries ...
(Date:4/16/2015)... April 16, 2015  In recognition of World Hemophilia Day, ... advocacy groups to illuminate more than 15 prominent landmarks red ... Boston,s Zakim Bridge and Prudential Tower, ... Mercedes-Benz Superdome and Denver,s ... evening of April 17, and coincide with hemophilia community events ...
(Date:4/16/2015)... 2015 /CNW/ - Bioenterprise Corporation is pleased to announce that ... Charlottetown , PEI.  Bioenterprise has established a ... Council for the commercialization of agricultural technologies and innovations. ... PEI ADAPT.  They have been at the heart of ... explains Dave Smardon , President & CEO of ...
Breaking Biology Technology:Proove Bisociences to Participate in the Buzz of BIO at the 2015 BIO International Convention 2Proove Bisociences to Participate in the Buzz of BIO at the 2015 BIO International Convention 3The David Round Company Helps Companies Meet OSHA Standards by Combining Safety, Ergonomics, and Productivity 2Biogen Shines A Light On Hemophilia Community By Illuminating U.S. Landmarks Red On World Hemophlia Day 2Biogen Shines A Light On Hemophilia Community By Illuminating U.S. Landmarks Red On World Hemophlia Day 3Biogen Shines A Light On Hemophilia Community By Illuminating U.S. Landmarks Red On World Hemophlia Day 4Bioenterprise Corporation Expands into the Maritimes 2
... Provides A Modern Venue to View, Customize, and Purchase One-of-A-Kind Art Pieces ... ... -- DNA 11, the innovators and creators of custom portraits made from ... the DNA 11 House . The gallery also marks the ...
... Va., Feb. 25 Edmond Scientific Company (ESC) ... team of Tony Mallia as Practice Director. Mr. ... to bear his experience of enterprise integration in successful ... services sectors. The core goal of this methodology is ...
... reconstructive and aesthetic plastic surgeryMEDFORD, Mass., Feb. ... medical device company developing silk-based biomaterial platforms ... received 510(k) clearance from the U.S. Food ... SeriScaffold (TM) silk-based, long term bioresorbable scaffold ...
Cached Biology Technology:DNA 11, Creators of DNA Art, Opens First Pop-up Gallery 2Edmond Scientific Company 'Spotlight on the Executive': 2Serica Technologies Receives FDA 510(k) Clearance for SeriScaffold(TM) Technology for Soft Tissue Repair 2Serica Technologies Receives FDA 510(k) Clearance for SeriScaffold(TM) Technology for Soft Tissue Repair 3Serica Technologies Receives FDA 510(k) Clearance for SeriScaffold(TM) Technology for Soft Tissue Repair 4
(Date:4/1/2015)... Medisafe ™, the leading global medication management platform with ... Android smartphones and tablets, announces today that ... allow patients to track and correlate their adherence directly ... be able to visualize in real-time how taking medication ... glucose levels and blood pressure. "Patients need ...
(Date:3/26/2015)... The Granite Club, ... club, today announced it has implemented a state-of-the-art access ... for members and staff, while restricting access to authorized ... selected FST,s IMID Access system because it has the ... and staff, in addition to unparalleled security," said ...
(Date:3/24/2015)... 24, 2015   Crossmatch ™, a leading ... that its U.are.U ® 4500 Fingerprint Readers ... Bros . to achieve PCI compliance for Point-of-Sale ... to the POS terminal, protecting the retailer and ... breaches. With one-third of worldwide ...
Breaking Biology News(10 mins):Medisafe Announces Launch of Biometric Integration, Enabling Patients to Stay Adherent to Their Meds and See Tangible Health Results 2Granite Club First Private Club in North America to Implement FST Biometrics Secure Access Solution, as Part of Multi-million Dollar Expansion 2Crossmatch Fingerprint Readers Help Retailer Prevent Data Breaches at Point-of-Sale 2
... in marine mammals, brains reveals that these animals are ... DDTs and PCBs, as well as emerging contaminants such ... lead author on the study currently in press and ... performed the research as a student in the Woods ...
... radio landscape has made it tough for Foghat to get ... to new research published in The American Naturalist . ... that the songs of white-crowned sparrows change over time in ... factors that drive the evolution of mating signals in birds. ...
... Institutes of Health is launching the first integrated, ... rare and neglected diseases. The $24 million program ... Rare and Neglected Diseases Program, or TRND. ... is unusual because TRND creates a drug development ...
Cached Biology News:Skip this cocktail party 2Skip this cocktail party 3Bird songs change with environment 2NIH announces new program to develop therapeutics for rare and neglected diseases 2NIH announces new program to develop therapeutics for rare and neglected diseases 3NIH announces new program to develop therapeutics for rare and neglected diseases 4NIH announces new program to develop therapeutics for rare and neglected diseases 5
Vertical Rack (five box vapor phase rack), includes 2.0" (5.1cm) boxes...
... The 1-D PAGE ... Kit for Targeted Protein ... SDS PolyAcrylamide Gel Electrophoresis ... chemistry, and cleavable-linker technology ...
Conjugate Quality Control Evaluation Specificity: Conjugate Quality Control Evaluation ...
Qa-1 (L-12)...
Biology Products: