Navigation Links
Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success

titated by real-time PCR.
5) Data are normalized using 18S rRNA levels.
6) The extent of target gene knockdown is expressed as a percent of mRNA remaining in cells treated with the gene-specific siRNA
compared to cells treated with a negative control siRNA (Silencer Negative Control siRNA #1).

Figure 2. Silencer siRNA Validation Data Generated Using Applied Biosystems TaqMan Gene Expression Assays. The indicated Silencer Validated siRNAs were transfected into HeLa Cells at 30 nM. RNA was isolated 48 hours later and analyzed by one-step qRT-PCR using the appropriate TaqMan Gene Expression Assay (results were normalized for input RNA amount using real-time data for 18S rRNA). The inset graphs show the reduction in target gene expression compared to cells transfected with an equal concentration of Silencer Negative Control #1.


Optimizing siRNA Transfection
Before siRNA experiments can be conducted to study gene function, analyze biological pathways, or validate drug targets, optimized siRNA delivery conditions must be identified and/or verified. The best way to test, optimize, and validate siRNA transfection or electroporation conditions is to use a highly effective, verified siRNA to an appropriate target and to monitor decreases in mRNA levels induced by delivery of the siRNA. Ambion uses qRT-PCR as the method of choice, and Applied Biosystems TaqMan Gene Expression Assays provide validated, easy-to-use primer-probe sets for this purpose (F
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Silencer Validated siRNAs
2. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
3. Five Ways to Produce siRNAs
4. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
5. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Deliver siRNAs Into Primary Cells
9. Optimize Transfection of siRNAs for RNAi
10. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
11. Test More siRNAs for Less
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Matched siRNAs and Assays Ambion Applied Biosystems RNAi Success

(Date:4/29/2015)... BRM) ("Biorem" or "the Company") today announced its results for the three ... financial statements and MD&A have been filed on SEDAR ( www.sedar.com ). ... 2014 , 2013 Revenue 3,968 , 5,121 ... 1,703 , 2,231 , 5,866 Operating  expenses ... , 4,562 Ebitda* 92 , 418 , ...
(Date:4/29/2015)... 29, 2015 Cambrex Corporation (NYSE: CBM ) ... on Friday, May 1, 2015 before the market opens. ... will host a conference call to discuss the financial results. ... , Friday, May 1, 2015 at 8:30 a.m. ... for U.S.1-719-325-2454 for International  Passcode:  7993632 Dial-in Replay: ...
(Date:4/29/2015)... The leading Regenerative Veterinary Medicine Company, VetStem ... cell therapy has processed more than 11,000 samples. ... successful treatment for horses with potentially fatal injuries to ... worldwide exclusive license for adipose-derived (fat derived) stem cell ... treated in January 2004. Shortly after, the first dogs ...
(Date:4/29/2015)... 2015 /CNW/ - Northern Biologics Inc., a privately held ... an exclusive, strategic collaboration with Celgene Corporation.  As ... US$30 million upfront cash payment. It will use ... antibodies, and will have the right to receive ... its portfolio. Under the agreement, ...
Breaking Biology Technology:Biorem Reports Results for Fourth Quarter 2014 and Year Ended December 31, 2014 2Biorem Reports Results for Fourth Quarter 2014 and Year Ended December 31, 2014 3Biorem Reports Results for Fourth Quarter 2014 and Year Ended December 31, 2014 4VetStem Biopharma -Regenerative Cell Therapy has processed over 11,000 samples 2Northern Biologics and Celgene Enter Strategic Collaboration 2
... is financing a materials science project being conducted jointly ... with the Jlich Research Center. The support is to ... will total 550,000. The project managers, Professor Dr. Angelika ... Physical Chemistry at Johannes Gutenberg University Mainz (JGU), were ...
... Elsevier,s Computers & Graphics announces the upcoming publication ... incorporate the Collage Authoring Environment, a workflow and publication platform ... Paper Grand Challenge. The call for submissions for the special ... the 3DOR Workshop. Collage Authoring Environment allows authors ...
... The Houston Technology Center (HTC), Texas, ... gala, "A Celebration of Entrepreneurs . "  The ... impact on the region,s economy; and developed lifesaving and ... Beth Williams, CEO of TechTrans International, Inc., as this ...
Cached Biology Technology:En route to a quantum computer 2Leading Technology Incubator Honors Extraordinary Entrepreneurs 2Leading Technology Incubator Honors Extraordinary Entrepreneurs 3
(Date:4/17/2015)... 17, 2015 Increasing ... technological advancement to drive biometric systems market in ...   According to a recently released ... &  Opportunities, 2020 ", biometric systems market in ... growth at CAGR of over 22% through 2020. ...
(Date:4/13/2015)... , April 13, 2015 ... TechSci Research, "Global Biometrics Market Forecast & Opportunities, 2020", ... a CAGR of around 14% till 2020. The ... for large scale implementation and review of biometric ... launch of new products with greater efficiency, are ...
(Date:4/9/2015)... JOSE, Calif. , April 9, 2015 ... human interface solutions, announced today that it will report ... on Thursday, April 23, 2015, after the close of ... for analysts and investors at 2:00 p.m. PT (5:00 ...   To participate on the live call, ...
Breaking Biology News(10 mins):Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 2Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 3Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2Synaptics to Report Third Quarter Results on April 23 2
... the Panama Canal currently underway has created a rare opportunity ... sensitive Central American rain forest habitats. Dr. Amy Berkov, ... (CCNY), is leading a research effort that could shed new ... "If you want to study biodiversity and conservation, you need ...
... grasslands and the spread of forests may have been the ... woolly mammoth, woolly rhino and cave lion, according to Durham ... the theory that human beings were the primary cause of ... increased pressure on habitats. The research is part of ...
... Alzheimer,s disease destroys brain cells and their connections ... that disrupt work, hobbies and daily life. Symptoms can ... in the United States as Namenda), which is currently ... part, developed by Stuart A. Lipton, M.D., Ph.D., Director ...
Cached Biology News:CCNY biologists study rainforest host-plant associations 2CCNY biologists study rainforest host-plant associations 3Dwindling green pastures, not hunting, may have killed off the mammoth 2Dwindling green pastures, not hunting, may have killed off the mammoth 3Study explains why Alzheimer's drug is both safe and effective 2
Buckets for tube holder inserts...
...
... Block MBS Start up kit. Includes configured ... choice of 2 MultiBlock satellite thermal ... blocks. User friendly software. Store/track ... height heated lid. Outstanding uniformity. ...
Glycerokinase; from Bacillus stearothermophilus...
Biology Products: