( ...)Mastercycler Gradient Quick Programming Guide

Main Menu USE ARROW KEYS TO SCROLL THROUGH MENUS AND PROGRAMS. PRESS ENTER TO SELECT HIGHLIGHTED CHOICE)Menu items are chosen by first positioning cursor to the choice desired, then press . This confirms choice or input data or changes display to next menu in hierarchy. (press enter) Position cursor to choose program. to start program immediately
(Immediately after program starts, if CNTRL TUBE command is part of program,
use to choose tube type then , enter volume and (press enter) EDIT (press enter) A. This allows you to edit the program that was lasted loaded.
( see New feature for programming notes) LOAD (press enter) A. Scroll to selected program and press enter. Allows
one to edit loaded program. ( see New for programming notes) STANDARD (press enter) A. Instrument Default Program Example. Use cursor to select
line to be modified. (see New for programming notes) NEW

1. CNTRL: use key to choose BLOCK or TUBE, press
2. LID: enter desired temp., use key to choose WAIT or NOWAIT, use key to Fix or Auto.
3. Use arrow keys or after each step to automatically proceed to next line
   Use key to choose program commands (temp, go to, beep,pause, hold,link);use to insert a line into a program
4. Use key to list options for each step (time increments, temperatureincrements, ramp rates, gradient feature).
5. Use to save.
6. key to choose letter , +/- to reverse, and to select YES or NO

DELETE A. Position cursor to desired program for deletion press ENTER. Program is now deleted from menu (press enter) Editor ------
Printer ------
Gradient -----
General -----
Display extension steps (ie temperature or time increment)
Print program, parameter steps or protocol during PCR run
Calculator to display selected temperature distribution across block
Clock---- set clock
Remote--- use RS232
Sound--- set sound
Etc.---- Start with [Main Menu or Last Menu]; Auto Restart Program temperature of heated lid and switch on or off, Use key to turn on lid
Temperature displayed as Nominal/ Actual Temperature, heated lid switched off
when a temperature value of 0C entered
(Note: Once program starts, program settings have priority; Lid heating cannot
be switched on or off while program is running.) Use for incubation of samples. Set temperature. Use key to turn incubation mode ON or OFF.
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