Navigation Links
Mammalian Expression Vectors with Interchangeable Selectable Markers

it would be ideal to be able to quickly and efficiently exchange drug-resistance elements without subcloning into such vectors.

Cre-Mediated Recombination

To eliminate conventional restriction digestion and ligation subcloning procedures, site-specific recombination is used to manipulate DNA fragments. Site-specific recombinases, such as the P1-derived Cre recombinase, catalyze the exchange of DNA located between specific recognition sequences.3,4,5 For Cre recombinase, the recognition site (LoxP) consists of two inverted 13-bp repeats and an intervening, 8-bp nonpalindromic region.3 Intermolecular and intramolecular recombination between the two sites can occur.

Exchange of Selectable Markers

Fig.1

Stratagenes Exchanger cloning system is based on Cre-mediated, site-specific recombination, which allows fast and efficient insertion of prefabricated, selectable DNA fragments into core expression vectors. The system consists of a pExchange core expression vector, drug-resistance modules and Cre recombinase protein. The pExchange core vectors are based on the pCMV-Script and pCMV-Tag vectors vectors (Figure 1) that contain a LoxP site that serves as the acceptor site for the eukaryotic selectable-marker cassettes and offer a variety of expression options. The hygromycin-, puromycin-, or neomycin-resistance modules (Figure 2) are designed to be inserted into the LoxP site of either core vector. Each cassette is linearized and flanked by LoxP sites. A luciferase-containing core vector control for verifying expression in any cell line is provided along with Epicurian Coli XL-1 Blue supercompetent cells for simplifying transformation. All components are available separately.

'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
2. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
3. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
4. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
5. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
6. A New Lambda Vector for Mammalian Expression
7. Mammalian and Bacterial Expression in One Vector
8. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
9. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
10. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
11. Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells
Post Your Comments:
(Date:9/6/2008)... A free paper helps visitors to electronica ...ultilingual help, electronica information availabl...rmation useful in planning a trip, online registra...es and events. It includes an extensive list of we... , Munich, Germany (PRWEB...
(Date:9/5/2008)...-- With the publication of a study led by Yuntao W...s Department of Molecular and Microbiology, the me...g how the human immunodeficiency virus (HIV) attac...ed by HIV, affected more than 33 million people wo...tion statistics. , In the Sept. 5 issue of the j...
(Date:9/5/2008)...t pilots push planes to explore their limits, rese... Technology (NIST) are probing the newest microsco...acy at the nanoscale. Better nanoscale measurement... production in the semiconductor and nanomanufactu...y uses helium ions to generate the signal used to ...
(Date:9/5/2008)...ood & Drug Administration (FDA) issued in July 200...t acknowledged that nanoscale materials potentiall... the agency and that those materials present chall...vant to product safety and effectiveness may chang...pating the potential for rapid commercialization i...
Breaking Biology Technology:Free White Paper Now Available: How to get the most from your visit to electronica 2008 in Munich 2Free White Paper Now Available: How to get the most from your visit to electronica 2008 in Munich 3Researchers decode viral process that prepares cells for HIV infection 2NIST studies how new helium ion microscope measures up 2Nanotechnology and the FDA: size matters 2
...n technology opportunities noted that the FDA sees...as critical for the long-term safety and integrity...al packages to be tracked, traced and authenticate...cturer to pharmacy. , ,The FDA also is evaluating...tification systems for medical device equipment, b...
...ternational a developer of software products for ...DC) marketplace, has announced LABELVIEW 8 label d...LVIEW for wireless bar code label printing. , ,Th...d LABELVIEW support for RFID applications and grea...r 1,000 devices. , ,TEKLYNX is headquartered in Mi...
... has announced the release of Centricity Physicia...puting platform for physicians that extends the fu...le devices such as PDAs and Smartphones. , , Cen... to access and manage their patient lists and sche...hysicians can wirelessly sync their devices to con...
...cilities, Inc. , a global leader in the design, de... networks, technology networks and security system...ovide network design and deployment services for t... Wi-Fi network. Working together with communicatio...with Madison Gas & Electric, WFI will deploy a Wi-...
Other Biology Technology:RFID medical devices - Opportunities and challenges 2RFID medical devices - Opportunities and challenges 3RFID medical devices - Opportunities and challenges 4
(Date:9/4/2008)...re are numerous plant-parasitic nematodes in the w...argest part of an estimated $157 billion in agricu...mall worms that burrow into plant roots and feed o...searchers have contributed to the release of the a...atodes: Meloidogyne incognita -- the southern ro...
(Date:9/4/2008)...alif. Subtle genetic changes that confer an evolut...rity characteristic of the human hand, while diffi...odel system, have nevertheless generated keen inte...published online in the September 5 edition of the...rtment of Energy,s Lawrence Berkeley National Labo...
(Date:9/4/2008)... 4, 2008 As the anniversary of the September 11 a...ACS) has issued a new podcast describing an array ... national security. The podcast is the sixth epis...y Solutions )series. , With new episodes posted ... problems facing society in the 21st Century, and ...
(Date:9/4/2008)...- Biological systems are constantly evolving in wa... environmental fluctuations and internal errors. N...University research team has found new evidence th...e especially well suited to handle potentially har..., The results, published online this week in the...
Breaking Biology News(10 mins):ISU researchers help map first plant-parasitic nematode genome sequence 2Thumbs up -- a tiny ancestral remnant lends developmental edge to humans 2Thumbs up -- a tiny ancestral remnant lends developmental edge to humans 3New 'chemical radar' among national security innovations in ACS podcast 2New 'chemical radar' among national security innovations in ACS podcast 3New evidence on the robustness of metabolic networks 2Study says COPD testing is not measuring up 24 1Study says COPD testing is not measuring up 24 2Drug for cluster headaches may cause heart problems 22 1Drug for cluster headaches may cause heart problems 22 2Antioxidants show no clear benefit against cardiovascular events death in high risk women 20 1Antioxidants show no clear benefit against cardiovascular events death in high risk women 20 2DNA vaccine against multiple sclerosis appears safe potentially beneficial 18 1DNA vaccine against multiple sclerosis appears safe potentially beneficial 18 2
...On Monday, the Harbor Branch drug discovery group ...its of Florida in search of organisms that produce...h as cancer and Alzheimer,s. The work will include...ay Sal Bank, which encompasses a number of small, ...l was once notorious for its role in illegal drug ...
...If you thought the sleep-deprived months with your...or killer whale. , Reporting in the June 30 editi...ans Affairs neuroscientists report a developmental...that is unique from other mammals, with calves of ...rst month. , The mother also has minimal sleep dur...
...Dutch researcher Sebastian Nijman has discovered n...cancer. The results of his research have led to a ...of cancer, cylindromatosis. , Patients with the v... a lot of benign tumours on the skin. These tumour...rious malformations. , People with this disease ha...
...In response to the growing number of lipids expect...cipation of the massive amounts of data that will ...nal group of scientists has developed a comprehens...resentation system for lipids. The details of the ...ipid Research, an American Society for Biochemistr...
Other Biology News:Searching the depths of the straits of Florida for disease cures 2Searching the depths of the straits of Florida for disease cures 3Unlike other mammals, newborn dolphins and orcas stay active 24/7 during first months of development 2Lipids get new comprehensive classification system 2Lipids get new comprehensive classification system 3
Taq DNA Polymerase (T. aquaticus), 250 units. For PCR and other procedures requiring DNA polymerase activity at elevated temperatures. Category: Nucleotides & Enzymes & Biochemicals, Modifying Enzyme
...units/µL ,• High specific activity an...Leaves an 'A' overhang ,• Processes ,A...igh activity in primer extension and other molecul...the enzyme, each lot has been functionally tested ...
Recombinant DNA polymerase from Thermus aquaticus expressed in E. coli; 5 units/L; 1 tube of 1, 000 units enzyme/tube w/Buffer II and MgCl2
...units/µL ,• High specific activity an...Leaves an 'A' overhang ,• Processes ,A ...gh activity in primer extension and other molecula...he enzyme, each lot has been functionally tested f...
Biology Products: