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Making the most of stem cells

New storage method amplifies cells available for science

ANAHEIM - Like many other kinds of cells used in biomedical research, human embryonic stem cells are stored and transported in a cryopreserved state, frozen to -320 degrees Fahrenheit, the temperature of their liquid nitrogen storage bath.

But when scientists thaw the cells for use in the lab, less than 1 percent awa......

Full article >>> e in dry, low-temperature conditions - the Wisconsin team was able to increase stem cell survival rates by more than an order of magnitude, with as many as 20 percent of a cell culture surviving the freezing-and-thawing process.

"By using the gel and adding the disaccharide to cells, you can increase their chances of survival," notes de Pablo, also a UW-Madison professor of chemical and b......

Full article >>> ield environment.

The work by Palecek, de Pablo and Ji, which was supported by a grant from the Defense Advanced Research Projects Agency (DARPA), is also scheduled for publication in an upcoming issue of the journal Biotechnology and Bioengineering. A patent for the technology has been applied for through the Wisconsin Alumni Research Foundation.


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'"/> By Terry Devitt 03/31/04

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