Navigation Links
M13/Phagemid DNA Extraction Protocol

r 12 minutes) PLG 15 ml Light tube.
  • Add an equal volume of Tris-buffer saturated Phenol (pH 8.0) to the sample in the PLG 15 ml tube, and mix thoroughly by repeated inversion. Centrifuge at 1500 x g for 5 minutes to separate the phases.
  • Add an equal volume of Phenol-Chloroforrn-Isoamyl Alcohol (PCI, 25:24:1) to the sample in the same PLG 15 ml tube and extract and centrifuge as in step 5.
  • Add an equal volume of Chloroform-Isoamyl Alcohol (CI, 24:1) to the sample in the same PLG 15 ml tube and extract and centrifuge as in step 4.
  • Quantitatively transfer final aqueous phase to a 2 mL microcentrifuge tube, add 3 volumes of a 25:1 mixture of absolute Ethanol:3 M Sodium Acetate (pH 5.2), and mix well.
  • Let stand for 1530 minutes at room temperature, and centrifuge for 20 minutes at 12,000 x g or greater at 4C.
  • Wash pellet twice with 0.5 ml 70% Ethanol and air-dry.
  • Resuspend pellet in 2050 l TE, pH 8.0.
  • References
    1. Current Protocols in Molecular Biology, Volume I. (1989) Ausubel, F.M. et al. (eds.). John Wiley and Sons, New York, NY pp.1.14.1 - 1.15.4.
    2. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning, A Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. pp.4.29-4.32.
    3. PLG Notes. (1992) Prime Report 4(2):

    '"/>

    Source:


    Page: All 1 2

    Related biology technology :

    1. M13/Phagemid DNA Extraction Protocol
    2. Lambda DNA Extraction Protocol
    3. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    4. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    5. Lambda DNA Extraction Protocol
    6. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    7. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    8. S.E.T.S. Swab Extraction Tube System
    9. InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C
    10. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG: Phagemid DNA Extraction Protocol

    (Date:1/14/2014)... York, NY (PRWEB) January 14, 2014 ... of Alliqua, Inc. (OTCQB: ALQA). Alliqua is an emerging ... to serve the wound care market. , Free report ... was restructured with a seasoned management team and Board, ...
    (Date:1/14/2014)... (PRWEB) January 14, 2014 In recent ... motives and methods in product development and promotion has ... industry. This mistrust, fueled by concerns about the insidious ... by reports of spectacular fines to the world’s biggest ...
    (Date:1/14/2014)... Cardiff, UK (PRWEB) January 13, 2014 ... leader with more than 20 years in the industry, ... and photonics . Hainsey will serve as the society’s ... have Dr. Hainsey join SPIE as our Technology Strategist, ...
    (Date:1/14/2014)... (PRWEB) January 14, 2014 BioMedomics, Inc. ... ultra-sensitive POC diagnostic platforms and novel disease specific POC tests, ... of financing netting a total of $690,000. The investment is ... in the company. This group of private investors has significant ...
    Breaking Biology Technology:EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 3Photonics R&D Leader Bob Hainsey Joins SPIE Technical Staff 2BioMedomics, Inc. Closes $690K Round of Financing 2
    ... Md., May 5, 2011 GenVec, Inc. (NASDAQ: GNVCD) today ... letter from The NASDAQ Stock Market ("NASDAQ") advising that it ... GenVec had received a notice of deficiency on ... compliance with NASDAQ Marketplace Rules because the bid price of ...
    ... Reportlinker.com announces that a new market research report ... Tumor Markers - Global Pipeline Analysis, Competitive Landscape ... http://www.reportlinker.com/p0490349/Tumor-Markers---Global-Pipeline-Analysis-Competitive-Landscape-and-Market-Forecasts-to-2017.html Tumor Markers - ... to 2017 Summary ...
    ... 4, 2011 Bacterin International Holdings, Inc. ("Bacterin") (NYSE Amex: ... material, today announced that its chairman and CEO, Guy Cook, ... Equity Conference at the Hard Rock Hotel in Hollywood, Florida, ... At the time of the presentation, a live audio ...
    Cached Biology Technology:GenVec Regains Compliance with NASDAQ Listing Requirements 2Reportlinker Adds Tumor Markers - Global Pipeline Analysis, Competitive Landscape and Market Forecasts to 2017 2Reportlinker Adds Tumor Markers - Global Pipeline Analysis, Competitive Landscape and Market Forecasts to 2017 3Reportlinker Adds Tumor Markers - Global Pipeline Analysis, Competitive Landscape and Market Forecasts to 2017 4Reportlinker Adds Tumor Markers - Global Pipeline Analysis, Competitive Landscape and Market Forecasts to 2017 5Reportlinker Adds Tumor Markers - Global Pipeline Analysis, Competitive Landscape and Market Forecasts to 2017 6Reportlinker Adds Tumor Markers - Global Pipeline Analysis, Competitive Landscape and Market Forecasts to 2017 7Reportlinker Adds Tumor Markers - Global Pipeline Analysis, Competitive Landscape and Market Forecasts to 2017 8Reportlinker Adds Tumor Markers - Global Pipeline Analysis, Competitive Landscape and Market Forecasts to 2017 9Reportlinker Adds Tumor Markers - Global Pipeline Analysis, Competitive Landscape and Market Forecasts to 2017 10Reportlinker Adds Tumor Markers - Global Pipeline Analysis, Competitive Landscape and Market Forecasts to 2017 11Reportlinker Adds Tumor Markers - Global Pipeline Analysis, Competitive Landscape and Market Forecasts to 2017 12Reportlinker Adds Tumor Markers - Global Pipeline Analysis, Competitive Landscape and Market Forecasts to 2017 13Reportlinker Adds Tumor Markers - Global Pipeline Analysis, Competitive Landscape and Market Forecasts to 2017 14Bacterin International to Present at Noble Financial Capital Markets' Seventh Annual Equity Conference on May 16, 2011 2
    (Date:7/10/2014)... are a big, bad sea scorpion. , One minute, ... any prey in sight. The next, thanks to a ... to trolling for weaker, soft-bodied animals you stumble upon ... giant pterygotid eurypterid, the largest arthropod that ever lived. ... journal Biology Letters , dramatically re-interprets the creature,s ...
    (Date:7/10/2014)... The controversy centres around a family of enzymes known ... heme group in their active site. At the centre ... which becomes oxidised (ferryl) when a reacting heme is ... that has remained unanswered for decades is whether this ... hydroxyl group (OH). Resolving this fundamental question has implications ...
    (Date:7/10/2014)... research from The University of Texas Health Science Center ... Real (IYG), a health education program designed to delay ... reduce dating violence behaviors among minority youth. , According ... percent of high school youth are victims of physical ... 20 percent are victims of emotional dating violence. Previous ...
    Breaking Biology News(10 mins):Extinct sea scorpion gets a Yale eye exam, with surprising results 2Neutron crystallography solves long-standing biological mystery 2Neutron crystallography solves long-standing biological mystery 3It's Your Game ... Keep It Real reduces dating violence among minority youth 2
    ... Brian Chen has been awarded a prestigious Sloan Research ... Formation, investigates a big subject: deciphering the assembly instructions ... neural circuits wire up with precision. One of the ... the correct synaptic contacts during its development when faced ...
    ... named Brontomerus mcintoshi , or "thunder-thighs" after its enormously powerful ... is described in a paper recently published in the journal ... the U.K. and the U.S. A member of the ... Brachiosaurus , Brontomerus may have used its powerful ...
    ... the crowd? Is that all I,ll ever be? ... Do you ... the Crowd" It may seem paradoxical, but being ... UCLA life scientists. Biologists Kimberly Pollard and Daniel ... alarm-call vocalizations in eight species of rodents that live in social ...
    Cached Biology News:Prestigious Sloan Fellowship awarded 2New 'thunder-thighs' dinosaur discovered 2New 'thunder-thighs' dinosaur discovered 3Even in a crowd, you remain unique, UCLA life scientists report 2
    This buffer is Specifically formulated to be used with Endogen's Poly-HRP Streptavidin...
    ... Analyst Software has been optimized for the ... allow the software to quickly and accurately ... well of an ELISA plate or each ... Software has been customized to incorporate standard ...
    ... An easy-to-use solution that enables fully automated ... 400 Hybridization Station has been designed to ... the throughput necessary to perform a wide ... performed in the range 485C; denaturations of ...
    The Piezo Power MicroDissection (PPMD) system is designed for rapid isolation of single cells or small cell areas...
    Biology Products: