Navigation Links
M13/Phagemid DNA Extraction Protocol

r 12 minutes) PLG 15 ml Light tube.
  • Add an equal volume of Tris-buffer saturated Phenol (pH 8.0) to the sample in the PLG 15 ml tube, and mix thoroughly by repeated inversion. Centrifuge at 1500 x g for 5 minutes to separate the phases.
  • Add an equal volume of Phenol-Chloroforrn-Isoamyl Alcohol (PCI, 25:24:1) to the sample in the same PLG 15 ml tube and extract and centrifuge as in step 5.
  • Add an equal volume of Chloroform-Isoamyl Alcohol (CI, 24:1) to the sample in the same PLG 15 ml tube and extract and centrifuge as in step 4.
  • Quantitatively transfer final aqueous phase to a 2 mL microcentrifuge tube, add 3 volumes of a 25:1 mixture of absolute Ethanol:3 M Sodium Acetate (pH 5.2), and mix well.
  • Let stand for 1530 minutes at room temperature, and centrifuge for 20 minutes at 12,000 x g or greater at 4C.
  • Wash pellet twice with 0.5 ml 70% Ethanol and air-dry.
  • Resuspend pellet in 2050 l TE, pH 8.0.
    • References
    1. Current Protocols in Molecular Biology, Volume I. (1989) Ausubel, F.M. et al. (eds.). John Wiley and Sons, New York, NY pp.1.14.1 - 1.15.4.
    2. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning, A Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. pp.4.29-4.32.
    3. PLG Notes. (1992) Prime Report 4(2):

    '"/>

    Source:


    Page: All 1 2

    Related biology technology :

    1. M13/Phagemid DNA Extraction Protocol
    2. Lambda DNA Extraction Protocol
    3. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    4. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    5. Lambda DNA Extraction Protocol
    6. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    7. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    8. S.E.T.S. Swab Extraction Tube System
    9. InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C
    10. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    		TAG: Phagemid DNA Extraction Protocol

    (Date:5/21/2013)... Milwaukee, Wisc. (PRWEB) May 21, 2013 ... equipment parts distributor , will be exhibiting for ... the Advancement of Medical Instrumentation (AAMI) 2013 Conference ... manage, purchase, support, distribute, and manufacture healthcare technologies. ... 1-3 in Long Beach, California. , The ...
    (Date:5/21/2013)... 2013 Clinverse, Inc. , ... clinical trials, announced today it has formed a ... Clinverse’s ClinPay® FLS eClinical commerce platform aggregates data ... SpendMD ™, Aggregate Spend Solutions’ recently-launched flagship ... and reporting transfer of value at investigator meetings ...
    (Date:5/21/2013)... 2013 As the R&D sector ... within the industry to deliver better products, faster. ... pharma companies often means turning big ideas into ... in collaboration with Sensidose AB and Recipharm, is ... products to market sooner. The partnership resulted in ...
    (Date:5/21/2013)... Md. , May 21, 2013  MacroGenics, ... 1 study of margetuximab (MGAH22), an Fc-modified chimeric ... tumors expressing the HER2 oncoprotein," will be presented ... Society of Clinical Oncology during the Developmental Therapeutics ... 3 at 4:15 – 4:30 PM.  The presentation ...
    Breaking Biology Technology:Alpha Source, Inc. to Exhibit at the AAMI 2013 Conference and Expo 2Clinverse, Inc. Announces Strategic Alliance with Aggregate Spend Solutions, LLC 2Clinverse, Inc. Announces Strategic Alliance with Aggregate Spend Solutions, LLC 3The Development of an Innovative Microtablet Dosage Form, a Success Story: Live Webinar Hosted by Xtalks 2MacroGenics Announces Margetuximab (MGAH22) Phase 1 Data Presentation at ASCO; Initiates Phase 2 Clinical Study to Evaluate Activity of Margetuximab in Patients with Metastatic Breast Cancer 2MacroGenics Announces Margetuximab (MGAH22) Phase 1 Data Presentation at ASCO; Initiates Phase 2 Clinical Study to Evaluate Activity of Margetuximab in Patients with Metastatic Breast Cancer 3
    ... preclinical and Phase 1 safety studies, and plans for ... hyperplasia (BPH) are to be presentedCINCINNATI, May 14 ... medicines for an aging global population, will lead off ... 19, 2009 at 9:00 AM in Atlanta, Georgia. Ausio ...
    ... vs. prior year -DENVER, May 14 Corgenix Medical Corporation ... diagnostic test kits, today filed its third quarter Form 10-Q ... sales have increased during the quarter ended March 31, 2009, ... the results highlighted by the report are as follows:, ...
    ... /PRNewswire-FirstCall/ - Oncothyreon Inc. (NASDAQ: ONTY ) (TSX: ... million or $0.13 per basic and diluted share for the ... of $5.1 million or $0.26 per basic and diluted share ... loss is primarily the result of the transfer of manufacturing ...
    Cached Biology Technology:Ausio Pharmaceuticals to Present at Bio(R) 2009 2Corgenix Reports Third Quarter Fiscal 2009 Financial Results 2Corgenix Reports Third Quarter Fiscal 2009 Financial Results 3Corgenix Reports Third Quarter Fiscal 2009 Financial Results 4Oncothyreon reports first quarter 2009 financial results 2Oncothyreon reports first quarter 2009 financial results 3Oncothyreon reports first quarter 2009 financial results 4Oncothyreon reports first quarter 2009 financial results 5
    (Date:5/21/2013)... Researchers at the University of Illinois at Chicago College of ... and stores fat -- its main energy source -- and ... disease, under a new $2 million, 4-year grant from the ... heart changes how it uses fuel for energy. These changes ... disease and are different in men and women, says E. ...
    (Date:5/21/2013)... charted the path of insulin action in cells in ... blueprint for understanding what goes wrong in diabetes. ... Professor David James from Sydney,s Garvan Institute of Medical ... of the prestigious journal Cell Metabolism . , ... very important role in the body because it helps ...
    (Date:5/20/2013)... is known about the effect of physical education (PE) ... University finds that increasing the amount of time that ... of obesity. , The study represents some of the ... youth obesity, and is forthcoming in the Journal ... of the study can be viewed at: http://www.sciencedirect.com/science/article/pii/S0167629613000556 ...
    Breaking Biology News(10 mins):Do men's and women's hearts burn fuel differently? 2Insight into the dazzling impact of insulin in cells 2Insight into the dazzling impact of insulin in cells 3Gym class reduces probability of obesity, study finds for first time 2
    ... and heavy smokers develop Alzheimers disease years earlier than ... heavily, according to research that will be presented at ... in Chicago, April 1219, 2008. These results are ... or eliminate heavy smoking and drinking, we could substantially ...
    ... The vitamin industry has long touted antioxidants as a ... diet, but a new review of studies found no ... review authors said that some antioxidants could increase risk ... the nutraceuticals industry, but an antioxidant researcher with the ...
    ... from the National Institute of Standards and Technology ... Ground have developed reliable methods based on DNA ... anthrax spores after prolonged storage. The techniques and ... reference standard for anthrax detection and decontamination. ...
    Cached Biology News:Alzheimer's starts earlier for heavy drinkers, smokers 2Antioxidant users don't live longer, analysis of studies concludes 2Antioxidant users don't live longer, analysis of studies concludes 3NIST, Army researchers pave the way for anthrax spore standards 2