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Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" ,,,)

ed of xanthine oxidase and xanthine.8 In addition to assaying for superoxide anion activity, we suppressed its activity by introducing superoxide dismutase (SOD). Since SOD catalyzes the dismutation of O2 radicals into O2 and H2O2, the addition of SOD to xanthine-xanthine oxidase solution results in a decreased O2 concentration, which, in turn, leads to decreased oxidation of luminol or the chemiluminescent signal. Figure 3 shows the chemiluminescence assay of O2 generated by the control xanthine-xanthine oxidase reaction.

Conclusions

LumiMax is a simple, sensitive, and unique kit for researchers performing luminol-enhanced chemiluminescent assays. It has many applications including clinical assessment of leukocytes in patients who have altered oxidative means to control infections and biochemical research involving the analysis of leukocyte signal transduction pathwayscomponents comprising NADPH oxidase and requirements of the respiratory burst. In addition, the assay may be used to study the generation of oxygen radicals in AIDS patients9 and control their opportunistic infections; to detect, measure, or monitor cell aging or damage in selected agricultural crops;10 and to monitor cell damage by pesticides or environmental pollution.

REFERENCES
  1. Tanaka, M. et al. (1994) J. Neurochem. 63(1): 266-270.
  2. Stohs, S.J. (1995) J. Basic Clin. Physiol. Pharmacol. 6 (3-4): 205-228.
  3. Kozumbo, W.J. et al. (1985) Chem-Biol. Interactions 54: 199-207.
  4. Irani, K. et al. (1997) Science 275: 1649-1652.
  5. Lomax, K.J. et al. (1989) Science 245: 409-412.
  6. Nakazawa, H. et al. (1985) Biochem. Pharmacol. 34(4): 481-490.
  7. Kimura,
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