Navigation Links
Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" ,,,)


Non-cytotoxic chemiluminescent enhancer for increased sensitivity

Luminol-enhanced Assay for Superoxide Anion (O2 )

Danny Q. Hoang
Stratagene

Lorraine C. Pfefferkorn
Dartmouth School of Medicine, Dept. of Microbiology, Hanover, NH

Stratagene introduces the LumiMax detection assay, a simple and unique chemiluminescent-based method to qualitatively detect the presence of superoxide anions in cell culture. In addition, the kit provides a specific enhancement reagent to amplify chemiluminescent signal output that is noncytotoxic and nondenaturing to live cells. Superoxide anion (O2 ), a potent oxidant, is a short-lived oxygen radical that is released into the extracellular environment of stimulated leukocytes (monocytes, macrophages, and polymorphonuclear leukocytes).1 Superoxide anion is synthesized when NADPH-oxidase, a plasma membrane enzyme complex containing cytochrome b 558, transfers an electron to molecular oxygen. Superoxide anions are implicated in several key cellular processes, including oxidative stress damage,2 tumor promotion,3 and, most recently, cell growth and DNA synthesis via their involvement in the cell signaling pathway of proto-oncogene ras.4

At present, superoxide may be detected by measuring the reduction of exogenously supplied cytochrome c,5 the uptake of oxygen from the medium (measured by the Clark electrode6), or luminol-mediated chemiluminescence.7 The first method has limited sensitivity and is complicated by the reoxidation of superoxide-reduced cytochrome c by contaminants and cell lysis. The oxygen-uptake method is not only less sensitive than cytochrome c reduction
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Organize Your Data from the Big Blue Mutation Assay
3. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
4. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
5. measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay
6. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
7. Using Validated siRNAs in Functional Genomic Assays
8. Nuclease Protection Assays: The Basics
9. RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay
10. Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A
11. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Luminol enhanced Assay for Superoxide Anion face Symbol

(Date:5/15/2008)...f., May 16 /-,Bayer HealthC..., Inc.,(Nasdaq: ONXX ) today announced more than ... of Clinical Oncology (ASCO) annual meeting demons...ablets in multiple tumor types as,a single agent o...O will expand upon and support the proven efficacy...
(Date:5/15/2008)...erminate Marketing Agreement with Triax Aest...erms, NAPA, Calif., May 15 /PRNewswire-FirstCall/...e Sciences company engaged in the development,of t...ng, today announced,financial results for the quar...iple to terminate its marketing agreement with Tri...
(Date:5/15/2008)...wire-FirstCall/ -- Indevus,Pharmaceuticals, Inc. (...ill,present at the Rodman & Renshaw 5th Annual Glo...p.m. local time (10:55 a.m. EDT) and at the 2008 U...nference on Thursday, May,29, 2008 at 10:00 a.m. l...be web cast live and may be accessed by visiting t...
(Date:5/15/2008)...re-FirstCall/ -- Hologic, Inc. (Nasdaq:,HOLX) toda...er, will be,presenting at the Citigroup Investment...May 21 - 22, 2008, at the Hilton Hotel in New York...o a live audio webcast of,Hologic,s investor prese...astern Time on the investor section of the Company...
Breaking Biology Technology:Bayer and Onyx Announce Nexavar Data Presentations at 44th American Society of Clinical Oncology Annual Meeting 2Bayer and Onyx Announce Nexavar Data Presentations at 44th American Society of Clinical Oncology Annual Meeting 3Bayer and Onyx Announce Nexavar Data Presentations at 44th American Society of Clinical Oncology Annual Meeting 4Bayer and Onyx Announce Nexavar Data Presentations at 44th American Society of Clinical Oncology Annual Meeting 5Bayer and Onyx Announce Nexavar Data Presentations at 44th American Society of Clinical Oncology Annual Meeting 6Bayer and Onyx Announce Nexavar Data Presentations at 44th American Society of Clinical Oncology Annual Meeting 7Senetek PLC Reports First Quarter 2008 Financial Results 2Senetek PLC Reports First Quarter 2008 Financial Results 3Indevus Pharmaceuticals Scheduled to Present at Upcoming Investor Conferences 2Indevus Pharmaceuticals Scheduled to Present at Upcoming Investor Conferences 3
...dison, Wis. In a marriage of complementary nanot... Corp. has joined forces with a British firm. , ,...tom probe company Oxford NanoScience Ltd. from the...disclosed, but the transaction makes Imago the wor...sident and CEO Tim Stultz. , ,The companies have ...
...sconsin Rapids, Wis. After a brief hiatus, Steve...nc., as president and chief operating officer, ef...in Rapids-based education software developer in Ja... as executive vice president and senior vice presi...l succeed founder Terrance D. Paul as president. P...
...icago The biotechnology industry has a job to do... a luncheon attended by thousands at the BIO Chica...uce the spread of infectious diseases, guard again...he world,s hungry, tap sources of renewable energy... on. , ,Clinton,s vision of a future without the s...
...lwaukee, Wis. - New sales to some prominent custo...yst International during the first quarter of 2006...res were not provided, but Catalyst said it comple...se sales, services business, and overall profitabi...y established in the fourth quarter of 2005. The c...
Other Biology Technology:Imago acquires synergistic British firm 2Clinton says world needs biofuels, GM crops, and more answers 2Clinton says world needs biofuels, GM crops, and more answers 3
(Date:5/14/2008)... on evolution will gather in Minnesota June 20 thr...annual gathering of evolutionary biologists. The c...entists to share research related to evolution, is...useum of Natural History, the university,s College... Science Education. , Headlining the event is Ol...
(Date:5/14/2008)...posed of Harvard students and alumni was among the...evelopment Marketplace competition, held in Accra,...obial fuel cell-based lighting systems suitable fo...prize. , According to the World Bank, as only 26...based electricity most residents rely upon dangero...
(Date:5/14/2008)...onfounded evolutionary biologists since Charles Da...ince parasites depend on their hosts for survival,...orgia and Emory University study of monarch butter...ide on them finds that the parasites strike a midd...ng rapidly and the costs to their hosts. The study...
(Date:5/14/2008)...rt stand-in for humans in medical research, thanks...ntical genes may behave differently in mouse and m... biologists Ben-Yang Liao and Jianzhi Zhang reveal...the use of mouse models in studying human disease,...dings of the National Academy of Sciences (PNAS). ...
Breaking Biology News(10 mins):University of Minnesota to host world's largest conference on evolution 2Undergraduates develop 'dirt-powered' microbial fuel cells to light Africa 2Undergraduates develop 'dirt-powered' microbial fuel cells to light Africa 3Monarch butterflies help explain why parasites harm hosts 2Monarch butterflies help explain why parasites harm hosts 3Mouse can do without man's most treasured genes 2Mouse can do without man's most treasured genes 3Abbott to Present at Lehman Brothers Healthcare Conference 3786 1Abbott to Present at Lehman Brothers Healthcare Conference 3786 2Spectral announces 2007 financial results 3785 1Spectral announces 2007 financial results 3785 2Spectral announces 2007 financial results 3785 3Spectral announces 2007 financial results 3785 4Spectral announces 2007 financial results 3785 5Spectral announces 2007 financial results 3785 6Spectral announces 2007 financial results 3785 7Spectral announces 2007 financial results 3785 8Spectral announces 2007 financial results 3785 9ERBE Perseveres Yet Again in Opposition to Dr Jerome Canady and is Awarded More Than One Million Dollars 13752 1ERBE Perseveres Yet Again in Opposition to Dr Jerome Canady and is Awarded More Than One Million Dollars 13752 2Moderate alcohol consumption in middle age can lower cardiac risk 13749 1Moderate alcohol consumption in middle age can lower cardiac risk 13749 2
...hts tend to have a much lower quality of life when...study published in the latest issue of the UK-base...ssed 118 children who had birth weights of 1500g o...70 born at normal weights to compare their quality...itive and social functions. , They discovered tha...
...eptor (EGFR), a so-called kinase protein, is often...ancer drugs inhibiting the activated EGFR kinase h...pecially lung cancer. In a new study in PLoS Medic... the mutations in the EGFR gene that occur in glio...chers sequenced the whole coding sequence of the E...
...uit fly can be a little mean sometimes ?especially...t over. And, like people, some flies have shorter ...arolina Sate University genetics department have i...in the fruit fly Drosophila melanogaster, pointing...al aggression in humans and other animals. , The...
...e New York-based Wildlife Conservation Society (WC...ently fitted high-tech GPS (Global Positioning Sys...to help protect one of Asia,s most bizarre-looking...nder two feet at the shoulder and weighing about 5...s its large nose, or proboscis, similar to a tapir...
Other Biology News:Very low birth weight linked to reduced quality of life in pre-school children 2Fruit fly aggression studies have relevance to humans, animals 2Fruit fly aggression studies have relevance to humans, animals 3Asia's odd-ball antelope gets collared 2
Kit containing positive control DNA all parts of the HIV-1 genome, HIV-1 negative control DNA, Primers SK38, SK39, SK19
...rom mouse, rat, and human sources that have been f...onto slides. After removing the wax, the slides ar...itu hybridization. The Hybrid-Ready tissue slides ...35S-labeled and non-radioactive probes, as well as...
Quantibody Human Angiogenesis Array 1 (1 slide) Class: Quantitative Antibody Arrays Product Group: Antibody Array
Vybrant Multicolor Cell-Labeling Kit *DiO DiI DiD solutions 1 mL each* from Molecular Probes (Invitrogen)
Biology Products: