Navigation Links
Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" ,,,)


Non-cytotoxic chemiluminescent enhancer for increased sensitivity

Luminol-enhanced Assay for Superoxide Anion (O2 )

Danny Q. Hoang
Stratagene

Lorraine C. Pfefferkorn
Dartmouth School of Medicine, Dept. of Microbiology, Hanover, NH

Stratagene introduces the LumiMax detection assay, a simple and unique chemiluminescent-based method to qualitatively detect the presence of superoxide anions in cell culture. In addition, the kit provides a specific enhancement reagent to amplify chemiluminescent signal output that is noncytotoxic and nondenaturing to live cells. Superoxide anion (O2 ), a potent oxidant, is a short-lived oxygen radical that is released into the extracellular environment of stimulated leukocytes (monocytes, macrophages, and polymorphonuclear leukocytes).1 Superoxide anion is synthesized when NADPH-oxidase, a plasma membrane enzyme complex containing cytochrome b 558, transfers an electron to molecular oxygen. Superoxide anions are implicated in several key cellular processes, including oxidative stress damage,2 tumor promotion,3 and, most recently, cell growth and DNA synthesis via their involvement in the cell signaling pathway of proto-oncogene ras.4

At present, superoxide may be detected by measuring the reduction of exogenously supplied cytochrome c,5 the uptake of oxygen from the medium (measured by the Clark electrode6), or luminol-mediated chemiluminescence.7 The first method has limited sensitivity and is complicated by the reoxidation of superoxide-reduced cytochrome c by contaminants and cell lysis. The oxygen-uptake method is not only less sensitive than cytochrome c reduction
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Organize Your Data from the Big Blue Mutation Assay
3. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
4. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
5. measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay
6. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
7. Using Validated siRNAs in Functional Genomic Assays
8. Nuclease Protection Assays: The Basics
9. RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay
10. Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A
11. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Luminol enhanced Assay for Superoxide Anion face Symbol

(Date:9/15/2014)... when a patient has sepsis, a life-threatening condition in ... -- often too fast for antibiotics to help. A ... by a team at Harvard,s Wyss Institute for Biologically ... sepsis. , "Even with the best current treatments, ... least 30 percent of the time," said Mike Super, ...
(Date:9/15/2014)... Bioptigen announced today the availability of ... optical device metrology and pre-clinical ophthalmic research markets. ... Contact Lens Metrology is designed for non-destructive, non-contact, full ... IOL structures, and donor tissues. The metrology ... in a hydration bath (saline), automated segmentation of anterior ...
(Date:9/15/2014)... 15, 2014 Research and Markets has ... by Product, by Services, by Technology, by Application - Global ... Whole Exome Sequencing (WES) is next-generation technology used for ... exon. The exome, which is the total of all exons ... and is actually converted into proteins after translation. It is ...
(Date:9/15/2014)... ANNAPOLIS, Maryland , 15. September 2014 /PRNewswire/ ... Week (IPAW) wird vom 12. bis 18. ... der Plasma Protein Therapeutics Association (PPTA) ... Ziele: , Das globale Bewusstsein für ... Den Beitrag von Plasmaspendern beim Retten und Verbessern ...
Breaking Biology Technology:Blood-cleansing biospleen device developed for sepsis therapy 2Blood-cleansing biospleen device developed for sepsis therapy 3Blood-cleansing biospleen device developed for sepsis therapy 4Bioptigen Introduces Deep Imaging OCT System for Contact Lens Metrology 2Global Whole Exome Sequencing (Systems, Kits, Library Preparation, Target Enrichment) Market by Product, by Services, by Technology, by Application - Forecast to 2018 2Global Whole Exome Sequencing (Systems, Kits, Library Preparation, Target Enrichment) Market by Product, by Services, by Technology, by Application - Forecast to 2018 3International Plasma Awareness Week feiert Spender und rückt seltene Krankheiten in den Mittelpunkt 2International Plasma Awareness Week feiert Spender und rückt seltene Krankheiten in den Mittelpunkt 3
... , BONITA SPRINGS, Fla., Nov. 18 ... the technology group The Linde Group have agreed ... to identify the optimum management of carbon dioxide (CO2) ... technology. This cooperation will see the companies join forces ...
... BALTIMORE, Nov. 18 Champions Biotechnology, Inc. ... with a predictive preclinical platform aimed at accelerating the ... established an exclusive licensing agreement with Yale University and ... cancer treatment. Scientists from Yale and Southern Research ...
... , BOTHELL, Wash., Nov. 18 BioLife Solutions, Inc. ... of proprietary cGMP hypothermic storage and cryopreservation media products for ... granted a Japanese patent for an application that includes claims ... cold temperatures used in biopreservation. The patent is titled " ...
Cached Biology Technology:Algenol Biofuels and The Linde Group Agree To Cooperate in CO2 and O2 Management For Biofuel Production From Algae 2Algenol Biofuels and The Linde Group Agree To Cooperate in CO2 and O2 Management For Biofuel Production From Algae 3Champions Biotechnology Enters into Licensing Agreement with Yale University and Southern Research Institute for the Repurposing of Bithionol for Oncology 2Champions Biotechnology Enters into Licensing Agreement with Yale University and Southern Research Institute for the Repurposing of Bithionol for Oncology 3Champions Biotechnology Enters into Licensing Agreement with Yale University and Southern Research Institute for the Repurposing of Bithionol for Oncology 4BioLife Solutions Granted Japanese Biopreservation Patent 2BioLife Solutions Granted Japanese Biopreservation Patent 3
(Date:9/16/2014)... certain traits from our parents that are pre-determined. But ... out by taking certain drugs or better yet, just ... of researchers at the Boston University School of Medicine ... , Epigenetics regulates gene expression in a reversible ... prevent permanent mutations or alterations within the gene themselves. ...
(Date:9/16/2014)... fall marks a new school year, a new football ... Space Station. In September, SpaceX,s Dragon spacecraft is scheduled ... and investigations as part of the company,s fourth contracted ... will be the third suite of research investigations sponsored ... Space (CASIS). With the role of managing the U.S. ...
(Date:9/16/2014)... peacock show its extravagant, colorful tail feathers in courtship ... used a special camera developed by an engineer at ... northern swordtail fish choose their mates based on a ... Texas at Austin used a bioinspired polarization camera developed ... & engineering at Washington University, to make the discovery. ...
Breaking Biology News(10 mins):Epigenetic drugs: A hope to treat cancer resistance and reduce cancer relapse? 2Epigenetic drugs: A hope to treat cancer resistance and reduce cancer relapse? 3CASIS research set for launch aboard SpaceX mission to space station 2CASIS research set for launch aboard SpaceX mission to space station 3Camera developed at WUSTL sheds light on mate choice of swordtail fish 2
... 2009 A canine chromosome 7 locus that confers ... identified through a collaboration between the Behavior Service at ... Medical Genetics at the University of Massachusetts Medical School ... Technology. The findings are published in the January 2010 ...
... lll. Researchers report that Helicobacter pylori , ... environment of the human stomach, directly activates an enzyme ... types of cancer, including gastric cancer. Chronic infection ... factor for several forms of gastric cancer, but researchers ...
... scientific conference has provided new evidence for the ... of spectacular marine life, including deadly jellyfish, basking ... ,South West Marine Ecosystems, meeting, held in Plymouth ... the scientific, conservation, fishing and eco-tourism sectors. The ...
Cached Biology News:Canine compulsive disorder gene identified in dogs 2Canine compulsive disorder gene identified in dogs 3Team finds link between stomach-cancer bug and cancer-promoting factor 2Team finds link between stomach-cancer bug and cancer-promoting factor 3Conference highlights impact of unsettled summer weather on UK marine life 2
... 100 Instrument System is ideal for ... Upstate's Beadlyte reagents. The Luminex 100 ... with software version 1.7, the XY ... Upstate offers $2000 in free Beadlyte ...
Peptide-affinity Purified Polyclonal Antibody to ICEBERG Peptide with sequence EEDPQLASKMGLH, from C Terminus of the protein sequence according to NP_067546...
A431 cells Source: Cell Lysate Form: Ready to Use Application: Western Blotting Control...
Madin-Darby bovine kidney (MDBK) cells Source: Cell Lysate Form: Ready to Use Application: Western Blotting Control...
Biology Products: