Navigation Links
Low Abundance cDNA Cloned Using Stratagenes Human Universal ,,, cDNA Library



A time-saving alternative to conventional library plating and screening

Anuradha Mehta Donna Driscoll

Department of Cell Biology, Cleveland Clinic Foundation, Cleveland, OH

We chose Stratagenes Human Universal cDNA Library 1 (HUCL) to isolate a full-length cDNA clone encoding a novel low-abundance protein called Apobec-1 Complementation Factor (ACF) for several important reasons: The simple two-step hybridization protocol allowed us to quickly screen a large number of clones, normalization increased the chance of finding low-abundance cDNAs, the librarys varied tissue base aided in isolating a protein that was widely expressed but only in small amounts, and the average size of the library clones ranged within ACFs expected sequence size. We successfully isolated ACF cDNA, which was crucial in allowing us to define the minimal protein requirements for the C to U editing of apolipoprotein-B mRNA.

Purification of proteins and identification of their cognate cDNAs are powerful tools for studying the molecular mechanisms underlying biological systems. Highly sensitive mass spectrometric techniques provide valuable peptide sequence information from very limited amounts of protein, which can be used to screen the wealth of information available in the NCBI databasesincluding both nonredundant and Expressed Sequence Tag (EST) databases. In the case of a novel protein, identi
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
2. Oil-Free PCR Using the Hot Top Assembly
3. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
4. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
5. Using XL1-Red Mutator Strain to Generate Esterase Variants
6. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
(Date:10/1/2014)... 30, 2014 In response to ... to address sales, training and consumer engagement challenges, ... innovative mobile applications to turn their clients’ visions ... latest customized mobile app creations, are now featured ... In partnership with Toyota, StudioPMG created a robust ...
(Date:10/1/2014)... The U.S. Department of Labor has ... Edwardsville as part of the Trade Adjustment Assistance Community ... co-administered by the Department of Labor and Department of ... Thomas E. Perez and Secretary of Education Arne Duncan ... grants to nearly 270 colleges across the country. ...
(Date:9/30/2014)... available in German . ... Mnchen (TUM) have demonstrated a new kind of building ... future computer chips could be based on three-dimensional arrangements ... enabling technology of the semiconductor industry CMOS fabrication ... researchers and collaborators at the University of Notre Dame ...
(Date:9/30/2014)... Sept. 30, 2014 Nematicide is a ... Nematodes are microscopic parasitic roundworms, found in massive ... other plants and animals. Nematicides have tended to ... properties promoting migration through the soil. A single ... plant-parasitic nematodes. With more than 10,000 species classified, ...
Breaking Biology Technology:Digital Agency Studio PMG Launches New Case Studies on Website 2U.S. Dept. of Labor Awards SIUE Nearly $10-Million Grant 2U.S. Dept. of Labor Awards SIUE Nearly $10-Million Grant 3U.S. Dept. of Labor Awards SIUE Nearly $10-Million Grant 4A new dimension for integrated circuits: 3-D nanomagnetic logic 2A new dimension for integrated circuits: 3-D nanomagnetic logic 3Nematicides Market by Types (Fumigants, Organophosphates, Carbamate and Bio-Nematicides), Applications (Agrochemicals, Industrial, and Others), and Geography (North America, Europe, Asia-Pacific & ROW) - Global Trends & Forecasts to 2019 2
... Calif. and MONROVIA, Calif., Jan. 6, 2011 Amgen ... today that they will collaborate to develop XmAb ® ... and CD32b.  XmAb5871 is currently in late-stage preclinical development ... Under the terms of the agreement, Amgen has the ...
... SAN FRANCISCO, Jan. 6, 2011 Vista Partners ... Therapeutics, Inc. (Nasdaq: OPXA ) and raised ... Silver, Principal Analyst at Vista Partners stated, "Opexa announced yesterday ... for the treatment of Multiple sclerosis , is now ...
... 6, 2011 BioInformatics, LLC announces the release of ... Technologies:  Market Insights for Life Science Suppliers (#10-005) This ... respond in a timely fashion to the rapidly changing ... LLC quantifies and characterizes the market for genetic analysis ...
Cached Biology Technology:Amgen and Xencor Enter Option Deal to Co-Develop Xencor's Novel Antibody for Autoimmune Diseases 2Amgen and Xencor Enter Option Deal to Co-Develop Xencor's Novel Antibody for Autoimmune Diseases 3Vista Partners Updates Coverage on Opexa Therapeutics, Inc.; Raises Target Price From $4.40 to $6.50 2BioInformatics, LLC New Market Report - Genomics Technologies 2
(Date:9/30/2014)... know what goes wrong with your muscles because of age, ... "normal" actually is. That,s where a new research report published ... Journal comes in. In the report, a team ... that can help scientists "see" which genes are active in ... never-before-detected gene activity and that men have approximately 400 more ...
(Date:9/30/2014)... release is available in German . ... their behavioural response to risky situations such as exposure ... Ornithology now found in a long-term study on different ... both metabolic rate and ambient temperature. High metabolic rates ... as in these scenarios birds were more likely to ...
(Date:9/30/2014)... a suite of perilous threats in today,s ocean. ... climate change, fragile coral ecosystems are disappearing at ... some species of corals surrounding the island of ... in their tropical environment: coral guard-crabs. New research ... Marine Station scientist Seabird McKeon and the museum,s ...
Breaking Biology News(10 mins):Scientists identify which genes are active in muscles of men and women 2Risky metabolism 2Smithsonian scientists discover coral's best defender against an army of sea stars 2
... , Jan. 24, 2013 /PRNewswire-iReach/ -- The companion diagnostics ... has become one of the most promising areas within ... companies are now entering the highly attractive market of ... developing a combination product. The explosive M&A trend in ...
... their mother,s diet during gestation and lactation were found to ... than those not exposed to the chemical, researchers at the ... BPA is a chemical most commonly found in the lining ... in many hard plastic bottles, including baby bottles, but many ...
... Researchers at the University of Pittsburgh School of ... key HIV protein. In findings published online today in ... this vulnerable spot could stop the virus from replicating, ... Previous research demonstrated that a small HIV protein ...
Cached Biology News:Global Companion Diagnostic Market Worth $19.3 billion by 2023: What it Takes to Become a Major Companion Diagnostic Player 2Global Companion Diagnostic Market Worth $19.3 billion by 2023: What it Takes to Become a Major Companion Diagnostic Player 3Global Companion Diagnostic Market Worth $19.3 billion by 2023: What it Takes to Become a Major Companion Diagnostic Player 4Global Companion Diagnostic Market Worth $19.3 billion by 2023: What it Takes to Become a Major Companion Diagnostic Player 5Female mice exposed to BPA by mothers show unexpected characteristics 2Female mice exposed to BPA by mothers show unexpected characteristics 3Pitt team finds 'Achilles Heel' of key HIV replication protein 2
Mouse monoclonal antibody to ALAS2 - aminolevulinate, delta-, synthase 2 (sideroblastic/hypochromic anemia)...
Mouse monoclonal antibody to PDK2 - pyruvate dehydrogenase kinase, isoenzyme 2...
Mouse monoclonal antibody raised against a partial recombinant FBXO24. NCBI Entrez Gene ID = FBXO24...
Mouse polyclonal antibody raised against a partial recombinant WRB. NCBI Entrez Gene ID = 7485...
Biology Products: