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LiquiChip Kits for bead-based cytokine and kinase assays

a, PKC, Erk-2, and GSK-. The LiquiChip assay procedure uses fluorescently labeled detection reagents to quantify kinase activity
with high sensitivity (Figure 4) and specificity, eliminating the need for radioactivity and its attendant handling problems.

Sensitive and Specific Kinase Detection Stimulation of Kinases in Jurkat Cells Figure 3 Detection of serine/threonine kinase activity using the LiquiChip Ser/Thr Kinase Kit. Figure 4 Cultured Jurkat cells grown at 37C and 5% CO2 were exposed to a range of kinase-inducing reagents for 18 hours. EGF, epidermal growth factor (concentration = 50 ng/ml in medium); PMA, phorbol 12-myristate 13-acetate (50 ng/ml); PHA, phytohemagglutinin (50 ng/ml); PMA+PHA (50 ng/ml each); BA, butyric acid (5 mM); LPS, lipopolysaccharide (250 ng/ml). Kinase activity was measured in singleplex LiquiChip Ser/Thr Kinase assays using undiluted lysates from treated and untreated cells. The increase in
fluorescent intensity due to treatment is shown.





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