a, PKC, Erk-2, and GSK-. The LiquiChip assay procedure uses fluorescently labeled detection reagents to quantify kinase activity
with high sensitivity (Figure 4) and specificity, eliminating the need for radioactivity and its attendant handling problems.

Sensitive and Specific Kinase Detection Stimulation of Kinases in Jurkat Cells Figure 3 Detection of serine/threonine kinase activity using the LiquiChip Ser/Thr Kinase Kit. Figure 4 Cultured Jurkat cells grown at 37C and 5% CO2 were exposed to a range of kinase-inducing reagents for 18 hours. EGF, epidermal growth factor (concentration = 50 ng/ml in medium); PMA, phorbol 12-myristate 13-acetate (50 ng/ml); PHA, phytohemagglutinin (50 ng/ml); PMA+PHA (50 ng/ml each); BA, butyric acid (5 mM); LPS, lipopolysaccharide (250 ng/ml). Kinase activity was measured in singleplex LiquiChip Ser/Thr Kinase assays using undiluted lysates from treated and untreated cells. The increase in
fluorescent intensity due to treatment is shown.





'"/>

Source:


Page: All 1 2 3
Related biology technology :

1. Setting up a kinase profiler with IMAP
2. Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases
3. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
4. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
5. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
6. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
7. The measurement of DNA condensation and mitochondrial changes during apoptosis and necrosis in cell based assays
8. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
9. Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System
10. Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System
11. DELFIA Protein Kinase assays.