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LiquiChip Kits for bead-based cytokine and kinase assays

s are detected using a mixture of biotinylated cytokine-specific monoclonal antibodies and StreptavidinPE (Figures 1 and 2). The specific bead code assigned to each of the 10 cytokines enables their unambiguous identification and quantification in the assay.

Table 1. Cytokines measured using LiquiChip 10-Cytokine Kits Sensitive and Highly Specific Detection of Cytokines Stimulation of Cytokines in Jurkat Cells Figure 1 Detection of cytokines using LiquiChip Cytokine Kits. Figure 2 Cultured Jurkat cells grown at 37C and 5% CO2 were exposed to 5 mM butyric acid for 18 hours. Cell lysates from treated and untreated cells were assayed using the LiquiChip Human 10-Cytokine Kit. The increase in fluorescent intensities due to treatment is shown. Kinase assay without radioactivity

The LiquiChip Ser/Thr Kinase Kit is based on the phosphorylation of myelin basic protein (MBP) immobilized on LiquiChip beads. After incubation with a sample, phosphorylation of serine and threonine residues in MBP is detected using antibodies specific for phosphoserine and phosphothreonine. These
antibodies are in turn detected using a biotinylated secondary antibody and streptavidinPE (Figure 3). The effect of kinase inhibitors can be measured by adding the inhibitor and a kinase to the assay. The LiquiChip Ser/Thr Kinase Kit enables quantification of numerous serine/threonine kinases, including JNK, p38-MAPK, SAPK 4, PKB-alph
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