Navigation Links
LiquiChip Kits for bead-based cytokine and kinase assays

The LiquiChip Human and Mouse 10-Cytokine Kits and Ser/Thr Kinase Kit provide fast and accurate quantification of a range of cytokines and serine/threonine kinases. LiquiChip 10-Cytokine Kits enable simultaneous multiplex assay of 10 different cytokines using bead-based xMAP technology. The LiquiChip Ser/Thr Kinase Kit enables highly sensitive assay of kinases without using radioactivity.

LiquiChip Kits offer:
  • Fast and sensitive assay on the LiquiChip workstation
  • Complete assay kits including beads, buffers, and detection reagents
  • Assay template software for fast startup
Fast and sensitive assay

Bead-based LiquiChip Cytokine and Kinase assays can be performed in a homogeneous mix-and-measure format with no wash steps, increasing throughput and reducing hands-on time. LiquiChip assay kits provide everything needed for the assay procedure including LiquiChip beads, buffers, and detection reagents enhancing sample-to-sample and plate-to-plate reproducibility. The multiplexing capability of the LiquiChip System enables comprehensive sample profiling in a single well, using a minimal amount of material.

Sensitive, multiplex human and mouse cytokine assays

LiquiChip Human and Mouse 10-Cytokine Kits enable fast, accurate, and simultaneous quantification down to picogram levels of 10 separate human or mouse cytokines in a single sample (Table 1). Antibodies that are highly specific for individual cytokines are immobilized on LiquiChip beads with different bead codes. Beads are added to samples, where cytokines bind to their respective antibody. Bead-bound cytokine s are detected using a mixture of biotinylated cytokine-specific monoclonal antibodies and StreptavidinPE (Figures 1 and 2). The specific bead code assigned to each of the 10 cytokines enables their unambiguous identification and quantification in the assay.

Table 1. Cytokines measured using LiquiChip 10-Cytokine Kits Sensitive and Highly Specific Detection of Cytokines Stimulation of Cytokines in Jurkat Cells Figure 1 Detection of cytokines using LiquiChip Cytokine Kits. Figure 2 Cultured Jurkat cells grown at 37C and 5% CO2 were exposed to 5 mM butyric acid for 18 hours. Cell lysates from treated and untreated cells were assayed using the LiquiChip Human 10-Cytokine Kit. The increase in fluorescent intensities due to treatment is shown. Kinase assay without radioactivity

The LiquiChip Ser/Thr Kinase Kit is based on the phosphorylation of myelin basic protein (MBP) immobilized on LiquiChip beads. After incubation with a sample, phosphorylation of serine and threonine residues in MBP is detected using antibodies specific for phosphoserine and phosphothreonine. These
antibodies are in turn detected using a biotinylated secondary antibody and streptavidinPE (Figure 3). The effect of kinase inhibitors can be measured by adding the inhibitor and a kinase to the assay. The LiquiChip Ser/Thr Kinase Kit enables quantification of numerous serine/threonine kinases, including JNK, p38-MAPK, SAPK 4, PKB-alph a, PKC, Erk-2, and GSK-. The LiquiChip assay procedure uses fluorescently labeled detection reagents to quantify kinase activity
with high sensitivity (Figure 4) and specificity, eliminating the need for radioactivity and its attendant handling problems.

Sensitive and Specific Kinase Detection Stimulation of Kinases in Jurkat Cells Figure 3 Detection of serine/threonine kinase activity using the LiquiChip Ser/Thr Kinase Kit. Figure 4 Cultured Jurkat cells grown at 37C and 5% CO2 were exposed to a range of kinase-inducing reagents for 18 hours. EGF, epidermal growth factor (concentration = 50 ng/ml in medium); PMA, phorbol 12-myristate 13-acetate (50 ng/ml); PHA, phytohemagglutinin (50 ng/ml); PMA+PHA (50 ng/ml each); BA, butyric acid (5 mM); LPS, lipopolysaccharide (250 ng/ml). Kinase activity was measured in singleplex LiquiChip Ser/Thr Kinase assays using undiluted lysates from treated and untreated cells. The increase in
fluorescent intensity due to treatment is shown.





'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Setting up a kinase profiler with IMAP
2. Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases
3. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
4. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
5. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
6. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
7. The measurement of DNA condensation and mitochondrial changes during apoptosis and necrosis in cell based assays
8. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
9. Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System
10. Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System
11. DELFIA Protein Kinase assays.
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... ... 23, 2016 , ... Mosio, a leader in clinical research ... Recruitment and Retention Tips.” Partnering with experienced clinical research professionals, Mosio revisits the ... tools, and strategies for clinical researchers. , “The landscape of how patients receive ...
(Date:6/23/2016)... 2016 /PRNewswire/ - FACIT has announced the creation ... biotechnology company, Propellon Therapeutics Inc. ("Propellon" or "the ... a portfolio of first-in-class WDR5 inhibitors for the ... WDR5 represent an exciting class of therapies, possessing ... for cancer patients. Substantial advances have been achieved ...
(Date:6/23/2016)... ... June 23, 2016 , ... Charm Sciences, Inc. is pleased ... received AOAC Research Institute approval 061601. , “This is another AOAC-RI approval of ... Salter, Vice President of Regulatory and Industrial Affairs. “The Peel Plate methods perform ...
(Date:6/23/2016)... , June 23, 2016   EpiBiome , a ... $1 million in debt financing from Silicon Valley Bank ... automation and to advance its drug development efforts, as ... facility. "SVB has been an incredible strategic ... services a traditional bank would provide," said Dr. ...
Breaking Biology Technology:
(Date:6/9/2016)... , June 9, 2016  Perkotek an innovation leader in attendance control systems ... seamlessly log work hours, for employers to make sure the right employees are actually ... http://photos.prnewswire.com/prnh/20160609/377486LOGO ... ... ...
(Date:6/2/2016)... -- The Department of Transport Management (DOTM) of ... US Dollar project, for the , Supply and ... and IT Infrastructure , to Decatur ... of Identity Management Solutions. Numerous renowned international vendors participated in ... was selected for the most compliant and innovative solution. ...
(Date:5/24/2016)... superior patient care by providing unparalleled technology to leaders of the medical imaging industry. ... recently added to the range of products distributed by Ampronix. Photo - ... ... ... ...
Breaking Biology News(10 mins):