( teria in a sensitive specificand c...)LightCycler foodproof Beer Screening Kit

teria in a sensitive, specific, and convenient way. All these criteria are met by the recently introduced LightCycler foodproof Beer Screening Kit.

Product Description



The LightCycler foodproof Beer Screening Kit is supplied in a ready-to-use format. It includes all reagents necessary for the amplification and detection of DNA of the most relevant beer-spoilage bacteria (Table). Additionally, the LightCycler foodproof Beer Screening Kit enables the identification of five of the most troublesome beer-spoilage bacteria via melting-curve analysis: Lactobacillus brevis, Lactobacillus lindneri, Pediococcus damnosus, Pediococcus inopinatus, and Megasphaera cerevisiae (Figure 1).



Assay Procedure

The beer sample is filtrated and the filtrate is transferred into an enrichment broth to allow the contaminants to multiply up to approximately 1,000 cells/ml. Thus, falsepositive signals from dead cells are omitted (for yeast and yeast-containing samples, an aliquot of the sample is transferred directly into the broth). Finally, the culture is centrifuged and the cell pellet is lysed using one of the three recommended sample preparation kits:

• ShortPrep foodproof III Kit,
• High Pure foodproof II Kit, or
• MagNA Pure LC DNA III Kit.
  
  

The isolated DNA and the LightCycler foodproof Beer Screening PCR-Master Mix are mixed and transferred into the capillary. The LightCycler foodproof Beer Screening PCR-Master Mix is a premixed and ready-touse reagent which contains all components required for the amplification and the detection of the bacterial target DNAs.'"/>

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