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LightCycler foodproof Beer Screening Kit

A New LightCycler Kit for the Detection of Beer-Spoilage Bacteria

Breweries make continuous efforts to ensure the highest quality of their goods. To guarantee the consistency of product quality, the different stages of beer production are monitored for the presence of spoilage microorganisms. A spoiled beer can be identified by cloudiness due to organisms or precipitated proteins, or by off flavors.

In general, beer is a very difficult medium for microorganisms to grow in. It contains alcohol, carbon dioxide, bitter substances, and a low amount of oxygen conditions that prevent growth or even survival of most organisms. Nevertheless, some microorganisms have adapted to these conditions.

Lactobacillus, Pediococcus, Pectinatus and Megasphaera are the most troublesome bacteria. From these genera, only a few species cause real damage to beer. Breweries have established many different methods to check for and to reduce potential contamination.

Most microbiological laboratories use conventional culture methods to detect the contamination. Beer samples are filtrated, and the filtrate is then transferred into a liquid and/or solid enrichment medium and finally incubated for several days (3 7 days). In case of a positive result, the laboratories perform additional analyses to identify the potentially harmful microorganism. The production needs to be kept on hold until the microbiological analysis is completed, which can take up to 14 days.

As this time-consuming conventional procedure has negative economic consequences, there is a great demand for rapid tests. Such a test must detect all relevant beer-spoilage bac
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